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LEM3 Lem3p [ Saccharomyces cerevisiae S288C ]

Gene ID: 855393, updated on 3-May-2025
Official Symbol
LEM3
Official Full Name
Lem3p
Primary source
SGD:S000005267
Locus tag
YNL323W
See related
AllianceGenome:SGD:S000005267; FungiDB:YNL323W; VEuPathDB:YNL323W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
BRE3; ROS3
Summary
Contributes to aminophospholipid flippase activity and phosphatidylcholine flippase activity. Involved in cell surface receptor signaling pathway; phospholipid translocation; and regulation of vacuole organization. Located in endoplasmic reticulum and plasma membrane. Part of phospholipid-translocating ATPase complex. Orthologous to several human genes including TMEM30B (transmembrane protein 30B). [provided by Alliance of Genome Resources, May 2025]
Orthologs
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See LEM3 in Genome Data Viewer
Location:
chromosome: XIV
Exon count:
1
Sequence:
Chromosome: XIV; NC_001146.8 (31944..33188)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene palmitoyltransferase PFA3 Neighboring gene phosphatidylinositol-3,5-bisphosphate 5-phosphatase Neighboring gene Kre1p Neighboring gene calcium/hydrogen antiporter

Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae. Ono Y, et al. Biosci Biotechnol Biochem, 2009 Mar 23. PMID 19270412
  2. A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae. Kato U, et al. J Biol Chem, 2002 Oct 4. PMID 12133835
  3. A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Sitcheran R, et al. Genetics, 2000 Nov. PMID 11063677, Free PMC Article
  4. Human TMEM30a promotes uptake of antitumor and bioactive choline phospholipids into mammalian cells. Chen R, et al. J Immunol, 2011 Mar 1. PMID 21289302, Free PMC Article
  5. Conformational changes of a phosphatidylcholine flippase in lipid membranes. Xu J, et al. Cell Rep, 2022 Mar 15. PMID 35294892

See all (98) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
    Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.
  2. Import of choline phospholipids into S. cerevisiae DeltaLem3 vector is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing that the proteins are orthologous.
    Title: Human TMEM30a promotes uptake of antitumor and bioactive choline phospholipids into mammalian cells.
  3. Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains.
    Title: Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae.
  4. The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
    Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
  5. Lem3p-Dnf1p complex may play a role distinct from its plasma membrane aminophospholipid translocase activity when it substitutes for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments.
    Title: Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast.
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Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
contributes_to aminophospholipid flippase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
contributes_to phosphatidylcholine flippase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within aminophospholipid translocation IEA
Inferred from Electronic Annotation
more info
  
involved_in cell surface receptor signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phospholipid translocation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of vacuole organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
is_active_in Golgi apparatus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm HDA PubMed 
located_in endoplasmic reticulum HDA PubMed 
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
part_of phospholipid-translocating ATPase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
Lem3p
NP_014076.1
  • Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    31944..33188
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183161.1 → NP_014076.1  TPA: Lem3p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014076.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W0M3, P42838
    UniProtKB/TrEMBL
    A6ZSF8, B3LPF6, C7GWR9, C8ZGK2, G2WLE7, N1NWZ5
    Conserved Domains (1) summary
    COG5035
    Location:11 → 414
    CDC50; Cell cycle control protein [Cell division and chromosome partitioning / Transcription / Signal transduction mechanisms]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P42838.1

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