ERG6 sterol 24-C-methyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ERG6
Gene description: sterol 24-C-methyltransferase
Primary source: SGD:S000004467
Locus tag: YML008C
See related: AllianceGenome:SGD:S000004467
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ISE1; LIS1; SED6; VID1
Summary: Enables sterol 24-C-methyltransferase activity. Involved in ergosterol biosynthetic process. Located in endoplasmic reticulum and lipid droplet. Orthologous to human METTL27 (methyltransferase like 27). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ERG6 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (251839..252990, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The endosomal sorting complex required for transport complex negatively regulates Erg6 degradation under specific glucose restriction conditions.
Title: The endosomal sorting complex required for transport complex negatively regulates Erg6 degradation under specific glucose restriction conditions.
Lis1 has two opposing modes of regulating dynein, being capable of inducing both low and high affinity for the microtubule; study shows that these opposing modes depend on the stoichiometry of Lis1 binding to dynein and that this stoichiometry is regulated by the nucleotide state of dynein's AAA3 domain.
Title: Lis1 Has Two Opposing Modes of Regulating Cytoplasmic Dynein.
Data show that the erg6Delta mutant strain is more sensitive to hyperosmotic shocks than is WT.
Title: Nature of sterols affects plasma membrane behavior and yeast survival during dehydration.
In the K6R substituted Hmg2 mutant, the squalene content was approximately 20-fold higher than in the wild-type EGY48 parental strain and ERG6 deletion did not further enhance squalene accumulation.
Title: Observations on squalene accumulation in Saccharomyces cerevisiae due to the manipulation of HMG2 and ERG6.
Overexpression of ERG6 and ERG2 led to an increased ergosterol level.
Title: [Regulation role of sterol C-24 methyltransferase and sterol C-8 isomerase in the ergosterol biosynthesis of Saccharomyces cerevisiae].
photolabeling & mutagenesis were performed on 24-C-sterol methyltransferase to elucidate location & role of active site residues involved in AdoMet binding & catalysis; results indicate that one or both of Cys128 & Pro133 are covalently bound to AdoMet
Title: Photoaffinity labeling and mutational analysis of 24-C-sterol methyltransferase defines the AdoMet binding site.
Erg28p may not only anchor the C-4 demethylation enzyme complex to the ER but also acts as a protein bridge to the Erg6p enzyme required for the next ergosterol biosynthetic step.
Title: The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p).

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the ERG6 gene: The ERG6 gene is conserved in S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice.
Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables sterol 24-C-methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables sterol 24-C-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables sterol 24-C-methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ergosterol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in ergosterol biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ergosterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in steroid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in steroid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in sterol biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info	PubMed
is_active_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in lipid droplet	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sterol 24-C-methyltransferase

NP_013706.1

EC 2.1.1.41

Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

251839..252990 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182363.1 → NP_013706.1 TPA: sterol 24-C-methyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013706.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZG6, P25087

UniProtKB/TrEMBL

A0A6A5PSP1, A6ZM54, C7GLC6, G2WK44, N1P5A5

Conserved Domains (3) summary

COG2230
Location:66 → 277

Cfa; Cyclopropane fatty-acyl-phospholipid synthase and related methyltransferases [Lipid transport and metabolism]

pfam08241
Location:124 → 222

Methyltransf_11; Methyltransferase domain

pfam08498
Location:306 → 368

Sterol_MT_C; Sterol methyltransferase C-terminal