RPB3 DNA-directed RNA polymerase II core subunit RPB3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPB3
Official Full Name: DNA-directed RNA polymerase II core subunit RPB3
Primary source: SGD:S000001283
Locus tag: YIL021W
See related: AllianceGenome:SGD:S000001283; FungiDB:YIL021W; VEuPathDB:YIL021W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to DNA-directed RNA polymerase activity and RNA-directed RNA polymerase activity. Involved in termination of RNA polymerase II transcription. Located in nucleoplasm. Part of RNA polymerase II, core complex. Orthologous to human POLR2C (RNA polymerase II subunit C). [provided by Alliance of Genome Resources, Jun 2025]
Orthologs: all
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Genomic context

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See RPB3 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (312905..313861)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A regulatory phosphorylation site on Mec1 controls chromatin occupancy of RNA polymerases during replication stress.
Title: A regulatory phosphorylation site on Mec1 controls chromatin occupancy of RNA polymerases during replication stress.
identifed direct physical interaction between the Rpb3 Pol II subunit and the Mediator subunit, Med17; findings suggest a direct interaction between Mediator and Pol II is generally required for transcription of class II genes in eukaryotes
Title: Direct interaction of RNA polymerase II and mediator required for transcription in vivo.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA-directed RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to DNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed RNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in termination of RNA polymerase II transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase II core subunit RPB3

NP_012243.3

RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

312905..313861

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179371.3 → NP_012243.3 TPA: DNA-directed RNA polymerase II core subunit RPB3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012243.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVQ8, P16370

UniProtKB/TrEMBL

A6ZVP6, B3LTM9, B5VKS0, C7GPW9, C8ZAM7, G2WGC3, N1P1P4

Conserved Domains (1) summary

cd07031
Location:6 → 265

RNAP_II_RPB3; RPB3 subunit of Eukaryotic RNA polymerase II