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TIM44 protein translocase subunit TIM44 [ Saccharomyces cerevisiae S288C ]

Gene ID: 854790, updated on 23-Apr-2026
Official Symbol
TIM44
Official Full Name
protein translocase subunit TIM44
Primary source
SGD:S000001284
Locus tag
YIL022W
See related
AllianceGenome:SGD:S000001284; FungiDB:YIL022W; VEuPathDB:YIL022W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
ISP45; MIM44; MPI1
Summary
Enables protein-folding chaperone binding activity and protein-macromolecule adaptor activity. Involved in protein import into mitochondrial matrix. Located in mitochondrion. Part of PAM complex, Tim23 associated import motor. Used to study oncocytic carcinoma of the thyroid. Orthologous to human TIMM44 (translocase of inner mitochondrial membrane 44). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See TIM44 in Genome Data Viewer
Location:
chromosome: IX
Exon count:
1
Sequence:
Chromosome: IX; NC_001141.2 (311165..312460)

Chromosome IX - NC_001141.2Genomic Context describing neighboring genes Neighboring gene Atg45p Neighboring gene Zn(2+) transporter YKE4 Neighboring gene DNA-directed RNA polymerase II core subunit RPB3 Neighboring gene 1-(5-phosphoribosyl)-5- ((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase HIS6

Genomic Sequence:
NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria. Maarse AC, et al. EMBO J, 1992 Oct. PMID 1396562, Free PMC Article
  2. Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane. Scherer PE, et al. Proc Natl Acad Sci U S A, 1992 Dec 15. PMID 1465421, Free PMC Article
  3. Separation of structural and dynamic functions of the mitochondrial translocase: Tim44 is crucial for the inner membrane import sites in translocation of tightly folded domains, but not of loosely folded preproteins. Bömer U, et al. EMBO J, 1998 Aug 3. PMID 9687491, Free PMC Article
  4. Interaction of the Tim44 C-terminal domain with negatively charged phospholipids. Marom M, et al. Biochemistry, 2009 Dec 1. PMID 19863062
  5. Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product. Horst M, et al. EMBO J, 1993 Aug. PMID 8344245, Free PMC Article

See all (145) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural basis of mitochondrial protein import by the TIM23 complex.
    Title: Structural basis of mitochondrial protein import by the TIM23 complex.
  2. Using site-specific in vivo crosslinking and genetic approaches in Saccharomyces cerevisiae, the authors found that both domains of Tim44 interact with the major matrix-exposed loop of Tim23, with the C-terminal domain (CTD) binding Tim17 as well.
    Title: Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23.
  3. These data suggest that the translocation channel and the import motor of the TIM23 complex communicate through rearrangements of the two domains of Tim44 that are stimulated by translocating proteins.
    Title: Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein.
  4. Tim23 mitochondrial motor components are tethered to translocon via the scaffold Tim44, with regulatory protein Pam17 having independent interaction site.
    Title: Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor.
  5. Genetic and biochemical analyses reveal synthetic interactions between PAM17 and TIM44.
    Title: Pam17 and Tim44 act sequentially in protein import into the mitochondrial matrix.
  6. Tim44 is not simply a scaffold for binding of motor subunits but plays a differential role in the recruitment of PAM modules to the inner membrane translocase.
    Title: Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase.
  7. The N-terminal 167-amino-acid segment of mature Tim44 is sufficient for both interaction with mtHsp70 and destabilization of a Tim44-mtHsp70 complex caused by client protein binding.
    Title: Residues of Tim44 involved in both association with the translocon of the inner mitochondrial membrane and regulation of mitochondrial Hsp70 tethering.
  8. binding of Tim44 to mHsp70 prevents the formation of a complex between the latter and Tim14/Pam18-Tim16/Pam16.
    Title: The interplay between components of the mitochondrial protein translocation motor studied using purified components.
  9. interactions between Tim44 and mtHsp70, controlled by polypeptide binding, are required for efficient translocation across the mitochondrial inner membrane
    Title: Regulated interactions of mtHsp70 with Tim44 at the translocon in the mitochondrial inner membrane.
  10. To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, yeast Tim44p has been crystallized.
    Title: Preliminary crystallographic studies of yeast mitochondrial peripheral membrane protein Tim44p.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-folding chaperone binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-folding chaperone binding IEA
Inferred from Electronic Annotation
more info
  
enables protein-folding chaperone binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein-folding chaperone binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-macromolecule adaptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-macromolecule adaptor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in intracellular protein transport NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein import into mitochondrial matrix IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein import into mitochondrial matrix IEA
Inferred from Electronic Annotation
more info
  
involved_in protein import into mitochondrial matrix IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of PAM complex, Tim23 associated import motor IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of PAM complex, Tim23 associated import motor IDA
Inferred from Direct Assay
more info
 PubMed 
part_of TIM23 mitochondrial import inner membrane translocase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extrinsic component of mitochondrial inner membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrial inner membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrial inner membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in mitochondrial inner membrane TAS
Traceable Author Statement
more info
  
located_in mitochondrion HDA PubMed 
Preferred Names
protein translocase subunit TIM44
NP_012242.1
  • Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001141.2 Reference assembly

    Range
    311165..312460
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179372.1 → NP_012242.1  protein translocase subunit TIM44

    See identical proteins and their annotated locations for NP_012242.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VVQ7, Q01852
    UniProtKB/TrEMBL
    A0ABF7PIV8, A6ZVP5, B3LTN0, B5VKR9, C7GPX0, G2WGC2, N1P463
    Conserved Domains (1) summary
    TIGR00984
    Location:56 → 421
    3a0801s03tim44; mitochondrial import inner membrane, translocase subunit
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q01852.1

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