FIS1 Fis1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: FIS1
Official Full Name: Fis1p
Primary source: SGD:S000001327
Locus tag: YIL065C
See related: AllianceGenome:SGD:S000001327; FungiDB:YIL065C; VEuPathDB:YIL065C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MDV2
Summary: Predicted to enable lipid binding activity and molecular adaptor activity. Involved in mitochondrial fission and peroxisome fission. Located in mitochondrial outer membrane and peroxisome. Orthologous to human FIS1 (fission, mitochondrial 1). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Genomic context

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See FIS1 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (241308..241775, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Positively charged patch at the carboxyl terminus of Fis1p is required for both membrane insertion and organelle specificity.
Title: Evidence for Amino Acid Snorkeling from a High-Resolution, In Vivo Analysis of Fis1 Tail-Anchor Insertion at the Mitochondrial Outer Membrane.
Fis1-E78A at lower expression levels recruits Dnm1 into mitochondrial punctate structures but fails to support normal fission. Thus, Fis1 makes multiple interactions that support Dnm1 puncta formation and may be essential after this step, supporting a revised model for assembly of the mitochondrial fission machinery.
Title: A Targeted Mutation Identified through pKa Measurements Indicates a Postrecruitment Role for Fis1 in Yeast Mitochondrial Fission.
The lifespan extension caused by deletion of FIS1 is mainly due to a defect in peroxisome fission and not caused by a block in mitochondrial fragmentation.
Title: Inhibition of peroxisome fission, but not mitochondrial fission, increases yeast chronological lifespan.
1.75 A resolution crystal structure of the Fis1 cytoplasmic domain from Saccharomyces cerevisiae is reported which adopts a tetratricopeptide-repeat fold
Title: The 1.75 Å resolution structure of fission protein Fis1 from Saccharomyces cerevisiae reveals elusive interactions of the autoinhibitory domain.
Charcot-Marie-Tooth-related gene GDAP1 complements cell cycle delay at G2/M phase in Saccharomyces cerevisiae fis1 gene-defective cells
Title: Charcot-Marie-Tooth-related gene GDAP1 complements cell cycle delay at G2/M phase in Saccharomyces cerevisiae fis1 gene-defective cells.
NMR measurements sensitive to dynamics occurring on a wide range of time scales (picoseconds to minutes) were used to assess whether the Fis1 arm is dynamic.
Title: Evidence for conformational heterogeneity of fission protein Fis1 from Saccharomyces cerevisiae.
Deletion of the FIS1 gene in yeast consistently results in acquisition of a secondary mutation that confers sensitivity to cell death.
Title: Fis1 deficiency selects for compensatory mutations responsible for cell death and growth control defects.
findings show that Dnm1p-dependent peroxisome fission requires Fis1p, Caf4p and Mdv1p; association of Caf4p and Mdv1p with peroxisomes relies on Fis1p
Title: Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p.
reveal how Fis1 recruits the mitochondrial fission complex and will facilitate efforts to manipulate mitochondrial fission
Title: Structural basis for recruitment of mitochondrial fission complexes by Fis1.
Refinement against the (13)C' T(1)/T(2) improved the overall quality of the yeast Fis1 structure, as evaluated by cross-validation against the residual dipolar coupling as well as the (15)N relaxation data.
Title: Refinement of protein structure against non-redundant carbonyl 13C NMR relaxation.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables lipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables molecular adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables molecular_function	ND No biological Data available more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial fission	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial fission	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peroxisome fission	IBA Inferred from Biological aspect of Ancestor more info
involved_in peroxisome fission	IGI Inferred from Genetic Interaction more info	PubMed
involved_in peroxisome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitochondrial fission	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrial outer membrane	HDA more info	PubMed
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
is_active_in peroxisomal membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Fis1p

NP_012199.3

Protein involved in mitochondrial fission and peroxisome abundance; may have a distinct role in tethering protein aggregates to mitochondria in order to retain them in the mother cell; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation