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RPB8 DNA-directed RNA polymerase core subunit RPB8 [ Saccharomyces cerevisiae S288C ]

Gene ID: 854399, updated on 11-Jun-2025
Official Symbol
RPB8
Official Full Name
DNA-directed RNA polymerase core subunit RPB8
Primary source
SGD:S000005750
Locus tag
YOR224C
See related
AllianceGenome:SGD:S000005750; FungiDB:YOR224C; VEuPathDB:YOR224C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Contributes to DNA-directed RNA polymerase activity and RNA-directed RNA polymerase activity. Involved in tRNA transcription by RNA polymerase III; transcription by RNA polymerase I; and transcription by RNA polymerase II. Part of RNA polymerase I complex; RNA polymerase II, core complex; and RNA polymerase III complex. Orthologous to human POLR2H (RNA polymerase II, I and III subunit H). [provided by Alliance of Genome Resources, Jun 2025]
Orthologs
all
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See RPB8 in Genome Data Viewer
Location:
chromosome: XV
Exon count:
1
Sequence:
Chromosome: XV; NC_001147.6 (760825..761265, complement)

Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene Dsc3p Neighboring gene putative iron-binding protein ISU2 Neighboring gene Her1p

Genomic Sequence:
NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Early evolution of eukaryotic DNA-dependent RNA polymerases. Kwapisz M, et al. Trends Genet, 2008 May. PMID 18384908
  2. Sequence divergence of the RNA polymerase shared subunit ABC14.5 (Rpb8) selectively affects RNA polymerase III assembly in Saccharomyces cerevisiae. Voutsina A, et al. Nucleic Acids Res, 1999 Feb 15. PMID 9927738, Free PMC Article
  3. Eukaryotic RNA polymerase subunit RPB8 is a new relative of the OB family. Krapp S, et al. Nat Struct Biol, 1998 Feb. PMID 9461075
  4. [Molecular evolution and structure of eukaryotic nuclear RNA polymerase subunits in light of the exon-intron organization of their genes]. Shpakovskiĭ GV, et al. Bioorg Khim, 1999 Nov. PMID 10645488
  5. GAIA: a gram-based interaction analysis tool--an approach for identifying interacting domains in yeast. Zhang KX, et al. BMC Bioinformatics, 2009 Jan 30. PMID 19208164, Free PMC Article

See all (156) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that Top2 is required redundantly with Top1 for optimal recruitment of RNA polymerase II at their promoters.
    Title: Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to DNA-directed RNA polymerase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to DNA-directed RNA polymerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-directed RNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to RNA-directed RNA polymerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in RNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA transcription by RNA polymerase III IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transcription by RNA polymerase I IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transcription by RNA polymerase I IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in transcription by RNA polymerase II IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in transcription by RNA polymerase III IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in transcription by RNA polymerase III IPI
Inferred from Physical Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA-directed RNA polymerase complex IEA
Inferred from Electronic Annotation
more info
  
part_of RNA polymerase I complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of RNA polymerase I complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of RNA polymerase II, core complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of RNA polymerase II, core complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of RNA polymerase III complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of RNA polymerase III complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
DNA-directed RNA polymerase core subunit RPB8
NP_014867.1
  • RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001147.6 Reference assembly

    Range
    760825..761265 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183643.1 → NP_014867.1  TPA: DNA-directed RNA polymerase core subunit RPB8 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014867.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W2S9, P20436
    UniProtKB/TrEMBL
    A6ZP66, B3LJP7, C7GWF9, C8ZGX8, G2WN95, N1NVW0
    Conserved Domains (1) summary
    pfam03870
    Location:10 → 145
    RNA_pol_Rpb8; RNA polymerase Rpb8
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P20436.1

Gene LinkOut

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