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ALE1 lysophospholipid acyltransferase [ Saccharomyces cerevisiae S288C ]

Gene ID: 854346, updated on 3-Jun-2026
Official Symbol
ALE1
Official Full Name
lysophospholipid acyltransferase
Primary source
SGD:S000005701
Locus tag
YOR175C
See related
AllianceGenome:SGD:S000005701; FungiDB:YOR175C; VEuPathDB:YOR175C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
LCA1; LPT1; SLC4
Summary
Enables 1-acylglycerol-3-phosphate O-acyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; and lysophosphatidylethanolamine acyltransferase activity. Involved in phosphatidylethanolamine biosynthetic process. Acts upstream of or within phosphatidylcholine acyl-chain remodeling and phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine. Located in endoplasmic reticulum. Is active in mitochondria-associated endoplasmic reticulum membrane contact site. Orthologous to several human genes including MBOAT2 (membrane bound O-acyltransferase domain containing 2). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See ALE1 in Genome Data Viewer
Location:
chromosome: XV
Exon count:
1
Sequence:
Chromosome: XV; NC_001147.6 (659815..661674, complement)

Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene 5'-(N(7)-methyl 5'-triphosphoguanosine)-(mRNA) diphosphatase Neighboring gene Med4p Neighboring gene ferrochelatase HEM15 Neighboring gene tRNA-Val

Genomic Sequence:
NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The yeast acylglycerol acyltransferase LCA1 is a key component of Lands cycle for phosphatidylcholine turnover. Chen Q, et al. FEBS Lett, 2007 Nov 27. PMID 17996202
  2. Identification and characterization of the major lysophosphatidylethanolamine acyltransferase in Saccharomyces cerevisiae. Riekhof WR, et al. J Biol Chem, 2007 Sep 28. PMID 17652094
  3. Acyl-CoA: lysophosphatidylcholine acyltransferase from diatom P. Tricornutum efficiently remodels phosphatidylcholine containing polyunsaturated fatty acids. Jasieniecka-Gazarkiewicz K, et al. Sci Rep, 2024 Dec 28. PMID 39730635, Free PMC Article
  4. Saccharomyces cerevisiae lysophospholipid acyltransferase, Lpt1, requires Asp146 and Glu297 for catalysis. Renauer P, et al. J Lipid Res, 2015 Nov. PMID 26382650, Free PMC Article
  5. The membrane-bound O-acyltransferase Ale1 transfers an acyl moiety to newly synthesized 2-alkyl-sn-glycero-3-phosphocholine in yeast. Morisada S, et al. FEBS Lett, 2018 Jun. PMID 29782033

See all (57) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lysophospholipid acyltransferase-mediated formation of saturated glycerophospholipids maintained cell membrane integrity for hypoxic adaptation.
    Title: Lysophospholipid acyltransferase-mediated formation of saturated glycerophospholipids maintained cell membrane integrity for hypoxic adaptation.
  2. results reveal that ALE1 is involved in acyl chain transfer to the sn-1 position of HHPC in yeast.
    Title: The membrane-bound O-acyltransferase Ale1 transfers an acyl moiety to newly synthesized 2-alkyl-sn-glycero-3-phosphocholine in yeast.
  3. D146 and E297 act in conjunction with H382 as nucleophiles that attack the hydroxyl group in lysophospholipids in a general acid/base mechanism in Lpt1.
    Title: Saccharomyces cerevisiae lysophospholipid acyltransferase, Lpt1, requires Asp146 and Glu297 for catalysis.
  4. pology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
    Title: Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
  5. The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
    Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
  6. Identification of LCA1 provides an essential molecular tool for further study of Lands cycle in phosphatidylcholine turnover
    Title: The yeast acylglycerol acyltransferase LCA1 is a key component of Lands cycle for phosphatidylcholine turnover.
  7. in concert with Slc1, Lpt1 plays a central role in Phosphatidic acid biosynthesis, which is essential for cell viability.
    Title: LPT1 encodes a membrane-bound O-acyltransferase involved in the acylation of lysophospholipids in the yeast Saccharomyces cerevisiae.
  8. Slc1p and Slc4p may not only be active as 1-acylglycerol-3-phosphate O-acyltransferases but also be involved in fatty acid exchange at the sn-2-position of mature glycerophospholipids.
    Title: SLC1 and SLC4 encode partially redundant acyl-coenzyme A 1-acylglycerol-3-phosphate O-acyltransferases of budding yeast.
  9. that a previously uncharacterized open reading frame, YOR175C, encodes the major acyl-CoA-dependent lysophosphatidylethanolamine acyltransferase activity in yeast
    Title: Identification and characterization of the major lysophosphatidylethanolamine acyltransferase in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables 1-acylglycerol-3-phosphate O-acyltransferase activity EXP
Inferred from Experiment
more info
 PubMed 
enables 1-acylglycerol-3-phosphate O-acyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 1-acylglycerol-3-phosphate O-acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables 1-acylglycerol-3-phosphate O-acyltransferase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables 1-acylglycerol-3-phosphate O-acyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables 1-acylglycerophosphocholine O-acyltransferase activity EXP
Inferred from Experiment
more info
 PubMed 
enables 1-acylglycerophosphocholine O-acyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 1-acylglycerophosphocholine O-acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables 1-acylglycerophosphocholine O-acyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables 1-acylglycerophosphoethanolamine O-acyltransferase activity EXP
Inferred from Experiment
more info
 PubMed 
enables 1-acylglycerophosphoethanolamine O-acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables 1-acylglycerophosphoserine O-acyltransferase activity EXP
Inferred from Experiment
more info
 PubMed 
enables 1-acylglycerophosphoserine O-acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables lysophosphatidylethanolamine acyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lysophosphatidylethanolamine acyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in glycerophospholipid biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glycerophospholipid biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in glycerophospholipid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in lipid modification IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within phosphatidylcholine acyl-chain remodeling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphatidylcholine biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in phosphatidylethanolamine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phospholipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in endoplasmic reticulum HDA PubMed 
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane EXP
Inferred from Experiment
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in mitochondria-associated endoplasmic reticulum membrane contact site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondria-associated endoplasmic reticulum membrane contact site IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
lysophospholipid acyltransferase
NP_014818.1
  • Broad-specificity lysophospholipid acyltransferase; major lysophosphatidylethanolamine acyltransferase that localizes to the mitochondria-associated ER membrane; member of the MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; capable of remodeling phosphatidylcholine containing VLC polyunsaturated fatty acids and involved in fatty acid exchange at the sn-2 position of mature glycerophospholipids

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001147.6 Reference assembly

    Range
    659815..661674 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183594.1 → NP_014818.1  lysophospholipid acyltransferase

    See identical proteins and their annotated locations for NP_014818.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W2N1, Q03130, Q08548
    UniProtKB/TrEMBL
    A6ZP18, C7GN52, C8ZGT6, E9P8E9, N1P3B1
    Conserved Domains (1) summary
    COG5202
    Location:1 → 563
    COG5202; Predicted membrane protein [Function unknown]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q08548.1

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