MDY2 Mdy2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MDY2
Official Full Name: Mdy2p
Primary source: SGD:S000005471
Locus tag: YOL111C
See related: AllianceGenome:SGD:S000005471; FungiDB:YOL111C; VEuPathDB:YOL111C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: GET5; TMA24
Summary: Enables protein-macromolecule adaptor activity. Involved in cell morphogenesis involved in conjugation with cellular fusion and post-translational protein targeting to endoplasmic reticulum membrane. Located in cytoplasmic stress granule and nucleus. Part of TRC complex. Orthologous to human UBL4A (ubiquitin like 4A) and UBL4B (ubiquitin like 4B). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See MDY2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (108258..108896, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast.
Title: Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast.
The crystal structure of the Get3-Get4-Get5 complex in an ATP-bound state is presented.
Title: Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4.
The nuclear history of the UBL Mdy2 appears to be essential for its function in cytoplasmic stress granules during the rapid cellular response to heat stress.
Title: Nuclear import of UBL-domain protein Mdy2 is required for heat-induced stress response in Saccharomyces cerevisiae.
Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.
Title: Structures of the Sgt2/SGTA dimerization domain with the Get5/UBL4A UBL domain reveal an interaction that forms a conserved dynamic interface.
solve a structure of the Sgt2_NT/Get5_UBL complex, validate it via site-directed mutagenesis, and empirically determine its stoichiometry using relaxation experiments and isothermal titration calorimetry
Title: Structure of the Sgt2/Get5 complex provides insights into GET-mediated targeting of tail-anchored membrane proteins.
Get5 carboxyl-terminal domain is a novel dimerization motif that tethers an extended Get4/Get5 complex.
Title: Get5 carboxyl-terminal domain is a novel dimerization motif that tethers an extended Get4/Get5 complex.
Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum
Title: Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum.
a structural model of the Sgt2-Get4/Get5-HSC complex.
Title: A structural model of the Sgt2 protein and its interactions with chaperones and the Get4/Get5 complex.
TA protein sorting defects are more severe in sgt2Delta get5Delta mutants compared to single knockouts
Title: Cooperative and independent activities of Sgt2 and Get5 in the targeting of tail-anchored proteins.
Data provide further evidence for a model in which Get4/5 operates upstream of Get3 and mediates the specific delivery of a TA substrate.
Title: Structural characterization of the Get4/Get5 complex and its interaction with Get3.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein-macromolecule adaptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell morphogenesis involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-translational protein targeting to endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in post-translational protein targeting to endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
involved_in protein insertion into ER membrane	HGI more info	PubMed

Component	Evidence Code	Pubs
part_of TRC complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Mdy2p

NP_014530.1

Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

108258..108896 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183365.1 → NP_014530.1 TPA: Mdy2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014530.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1V6, Q12285

UniProtKB/TrEMBL

A6ZN99, B3LIV4, B5VRE6, C7GSS5, C8ZHK4, G2WMC5, N1NZ23

Conserved Domains (3) summary

cd01805
Location:74 → 148

Ubl_Rad23; ubiquitin-like (Ubl) domain found in the Rad23 protein family

pfam16843
Location:8 → 59

Get5_bdg; Binding domain to Get4 on Get5, Golgi to ER traffic protein

pfam18514
Location:174 → 212

Get5_C; Get5 C-terminal domain