DYN1 dynein heavy chain [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: DYN1
Gene description: dynein heavy chain
Primary source: SGD:S000001762
Locus tag: YKR054C
Gene type: protein coding
RNA name: dynein heavy chain
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DHC1; PAC6

Genomic context

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See DYN1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (535647..547925, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Interplay among dynein, dynactin, and the stability of the microtubule substrate creates a mechanism that regulates accurate spindle positioning.
Title: Dynein is regulated by the stability of its microtubule track.
Lis1 (Pac1) regulates dynein by sterically blocking its mechanochemical cycle.
Title: Lis1 regulates dynein by sterically blocking its mechanochemical cycle.
DYN1 is engaged in bidirectional helical motility around microtubules in the process of cargo transport.
Title: Bidirectional helical motility of cytoplasmic dynein around microtubules.
Study reports X-ray and electron microscopy structures of yeast dynein, DYN1 bound to different ATP analogs, which collectively provide insight into the roles of dynein's two major ATPase sites, AAA1 and AAA3, in the conformational change mechanism.
Title: Allosteric communication in the dynein motor domain.
Results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells.
Title: Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body.
The authors find that dynein has a highly variable stepping pattern that is distinct from all other processive cytoskeletal motors, which use 'hand-over-hand' mechanisms.
Title: Dynein achieves processive motion using both stochastic and coordinated stepping.
study presents a 6 angstrom crystal structure of a functional dimer of two ~300-kilodalton motor domains of cytoplasmic dynein
Title: Crystal structure of the dynein motor domain.
Data suggest that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex.
Title: Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment.
Results suggest that dynein and Cin8 cooperate in anaphase midzone organization and influence microtubule dynamics, thus enabling progression through the slow phase of anaphase B.
Title: Mid-anaphase arrest in S. cerevisiae cells eliminated for the function of Cin8 and dynein.
the nucleotide binding state at AAA3 and AAA4 of the heavy chain of cytoplasmic dynein can allosterically modulate microtubule binding affinity and affect dynein processivity and force production
Title: Regulatory ATPase sites of cytoplasmic dynein affect processivity and force generation.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from BioSystems

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Phagosome, organism-specific biosystem (from KEGG)
Phagosome, organism-specific biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...
Phagosome, conserved biosystem (from KEGG)
Phagosome, conserved biosystemPhagocytosis is the process of taking in relatively large particles by a cell, and is a central mechanism in the tissue remodeling, inflammation, and defense against infectious agents. A phagosome is...

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the DYN1 gene: The DYN1 gene is conserved in S.cerevisiae, K.lactis, and E.gossypii.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IDA Inferred from Direct Assay more info	PubMed
ATP binding	IEA Inferred from Electronic Annotation more info
ATP-dependent microtubule motor activity, minus-end-directed	IBA Inferred from Biological aspect of Ancestor more info	PubMed
ATP-dependent microtubule motor activity, minus-end-directed	IDA Inferred from Direct Assay more info	PubMed
ATP-dependent microtubule motor activity, minus-end-directed	IMP Inferred from Mutant Phenotype more info	PubMed
ATPase activity	IEA Inferred from Electronic Annotation more info
dynein intermediate chain binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
dynein light chain binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
dynein light intermediate chain binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule motor activity	IEA Inferred from Electronic Annotation more info
motor activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
cytoplasmic microtubule organization	IBA Inferred from Biological aspect of Ancestor more info	PubMed
establishment of mitotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
establishment of mitotic spindle orientation	IGI Inferred from Genetic Interaction more info	PubMed
establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
karyogamy	IEA Inferred from Electronic Annotation more info
microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule-based movement	IEA Inferred from Electronic Annotation more info
minus-end-directed vesicle transport along microtubule	IBA Inferred from Biological aspect of Ancestor more info	PubMed
mitotic sister chromatid segregation	IGI Inferred from Genetic Interaction more info	PubMed
nuclear migration along microtubule	IGI Inferred from Genetic Interaction more info	PubMed
nuclear migration along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
astral microtubule	IDA Inferred from Direct Assay more info	PubMed
cell cortex	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IEA Inferred from Electronic Annotation more info
cytoplasmic dynein complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
cytoplasmic dynein complex	IPI Inferred from Physical Interaction more info	PubMed
cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
cytoskeleton	IEA Inferred from Electronic Annotation more info
dynein complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
dynein complex	IEA Inferred from Electronic Annotation more info
microtubule	IEA Inferred from Electronic Annotation more info
spindle pole body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dynein heavy chain

NP_012980.1

Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

535647..547925 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179844.1 → NP_012980.1 TPA: dynein heavy chain [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012980.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P36022

Conserved Domains (7) summary

COG5245
Location:774 → 4083

DYN1; Dynein, heavy chain [Cytoskeleton]

pfam03028
Location:3794 → 4016

Dynein_heavy; Dynein heavy chain and region D6 of dynein motor

pfam08385
Location:218 → 764

DHC_N1; Dynein heavy chain, N-terminal region 1

pfam08393
Location:1224 → 1627

DHC_N2; Dynein heavy chain, N-terminal region 2

pfam12777
Location:3014 → 3346

MT; Microtubule-binding stalk of dynein motor

pfam12781
Location:3378 → 3593

AAA_9; ATP-binding dynein motor region D5

cl21455
Location:1758 → 1977

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases