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TOF2 Tof2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853880, updated on 24-Jan-2026
Official Symbol
TOF2
Official Full Name
Tof2p
Primary source
SGD:S000001718
Locus tag
YKR010C
See related
AllianceGenome:SGD:S000001718; FungiDB:YKR010C; VEuPathDB:YKR010C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables phosphatase activator activity. Involved in protein localization to nucleolar rDNA repeats; rDNA chromatin condensation; and rDNA heterochromatin formation. Located in nucleolus. [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See TOF2 in Genome Data Viewer
Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (458924..461239, complement)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene bifunctional hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase FOX2 Neighboring gene tRNA-Leu Neighboring gene chromatin-associated RNAPIII regulator FPT1 Neighboring gene sterol-binding protein

Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The polySUMOylation axis promotes nucleolar release of Tof2 for mitotic exit. Gutierrez-Morton E, et al. Cell Rep, 2024 Jul 23. PMID 39002125, Free PMC Article
  2. Reversible Top1 cleavage complexes are stabilized strand-specifically at the ribosomal replication fork barrier and contribute to ribosomal DNA stability. Krawczyk C, et al. Nucleic Acids Res, 2014 Apr. PMID 24574527, Free PMC Article
  3. Identification and characterization of the genes for two topoisomerase I-interacting proteins from Saccharomyces cerevisiae. Park H, et al. Yeast, 1999 Jan 15. PMID 10028183
  4. A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis. Geil C, et al. Curr Biol, 2008 Jul 8. PMID 18595708
  5. Mechanism of Regulation of Intrachromatid Recombination and Long-Range Chromosome Interactions in Saccharomyces cerevisiae. Zaman S, et al. Mol Cell Biol, 2016 May 15. PMID 26951198, Free PMC Article

See all (72) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The authors show that Siz2 is enriched at the replication fork barrier (RFB) in the ribosomal DNA (rDNA) and also controls the homeostasis of Tof2 protein. siz2Delta resulted in increased accumulation of total Tof2 in the cell and a consequent increase in the enrichment of Tof2 at the rDNA.
    Title: Siz2 Prevents Ribosomal DNA Recombination by Modulating Levels of Tof2 in Saccharomyces cerevisiae.
  2. Ctf4-dependent recruitment of Dna2/Tof2 couples other processes to DNA synthesis, including rDNA copy-number regulation.
    Title: Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.
  3. These data support a model whereby Tof2 coordinates the biphasic release of Cdc14 during anaphase by restraining a population of Cdc14 in the nucleolus after activation of the Cdc14 early anaphase release (FEAR) network, for subsequent release by the MEN.
    Title: Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit.
  4. collaborates with Cdc14 specifically in rDNA segregation, presumably by targeting Cdc14 phosphatase activity to the nucleolus during anaphase to support resolution and compaction of this repetitive and highly transcribed DNA locus
    Title: A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables phosphatase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphatase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rDNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in nucleolus organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in nucleolus organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein localization to nucleolar rDNA repeats IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rDNA chromatin condensation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rDNA heterochromatin formation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in rDNA heterochromatin formation IEA
Inferred from Electronic Annotation
more info
  
involved_in rDNA heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in mitochondrion HDA PubMed 
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
Tof2p
NP_012935.3
  • Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    458924..461239 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179800.3 → NP_012935.3  Tof2p

    See identical proteins and their annotated locations for NP_012935.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VX76, Q02208
    UniProtKB/TrEMBL
    B3LR95, C7GNP7
    Conserved Domains (1) summary
    pfam10407
    Location:83 → 153
    Cytokin_check_N; Cdc14 phosphatase binding protein N-terminus
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q02208.1

Gene LinkOut

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