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TRIM5 tripartite motif containing 5 [ Homo sapiens (human) ]

Gene ID: 85363, updated on 6-Sep-2021

Summary

Official Symbol
TRIM5provided by HGNC
Official Full Name
tripartite motif containing 5provided by HGNC
Primary source
HGNC:HGNC:16276
See related
Ensembl:ENSG00000132256 MIM:608487
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RNF88; TRIM5alpha
Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]
Expression
Ubiquitous expression in urinary bladder (RPKM 5.4), endometrium (RPKM 5.4) and 25 other tissues See more
Orthologs
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Genomic context

See TRIM5 in Genome Data Viewer
Location:
11p15.4
Exon count:
12
Annotation release Status Assembly Chr Location
109.20210514 current GRCh38.p13 (GCF_000001405.39) 11 NC_000011.10 (5590906..5685108, complement)
105.20201022 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (5684425..5706304, complement)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene heterogeneous nuclear ribonucleoprotein A1 pseudogene 53 Neighboring gene Sharpr-MPRA regulatory region 4920 Neighboring gene olfactory receptor family 52 subfamily B member 6 Neighboring gene TRIM6-TRIM34 readthrough Neighboring gene Sharpr-MPRA regulatory region 4679 Neighboring gene tripartite motif containing 6 Neighboring gene tripartite motif containing 34 Neighboring gene uncharacterized LOC107984302 Neighboring gene tripartite motif containing 22 Neighboring gene olfactory receptor family 52 subfamily U member 1 pseudogene

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

Associated conditions

Description Tests
GWAS of DNA methylation variation within imprinting control regions suggests parent-of-origin association.
GeneReviews: Not available
Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
GeneReviews: Not available

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 replication is inhibited by TRIM5 expression in Jurkat T cells PubMed
TRIM5 (TRIM5alpha) R332G-R335G mutant protein efficiently restricts HIV-1 IIIB infection spreading/replicaton in CEM.NKR-CCR5 cells PubMed
Female Pumwani sex workers with the TRIM5 R136Q polymorphism (rs10838525) were less likely to seroconvert despite repeated heavy exposure to HIV-1. PubMed
Higher TRIM5 (TRIM5alpha) H43Y homozygotes reported in sero-negative Chinese intravenous drug users than in HIV-infected drug users; HIV-1 infection/transmission is restricted by TRIM5 43Y variant [in a Chinese intravenous drug user cohort] PubMed
TRIM5 (TRIM5alpha) 43Y allele was found less frequently in Japanese and Indian HIV-1-infected individuals relative to ethnicity-matched controls PubMed
TRIM5 (TRIM5alpha) 43Y vairant protects against HIV-1 transmisssion in African-Americans; HIV-1 is restricted by TRIM5 43Y variant PubMed
HIV/tuberculosis coinfection upregulates TRIM5 expression in pleural fluid mononuclear cells (PFMC) isolated from antiretroviral-naive coinfected patients (relative to patients infected with tuberculosis only) PubMed

Protein interactions

Protein Gene Interaction Pubs
Pr55(Gag) gag The viruses expressing Gag proteins from clinical isolates show differences in their sensitivity to TRIM5alpha, which is dependent on the TRIM5alpha alleles that the virus encounters PubMed
Tat tat Overexpression of HIV-1 Tat results in a nonspecific activation of the expression of TRIM5alpha PubMed
Vpu vpu The expression of TRIM5 is enhanced in Vpu-deficient HIV-1-infected cells as compared to that in wild-type-infected cells PubMed
capsid gag HIV-1 infection is modestly restricted by human TRIM5alpha, which acts on the incoming HIV-1 capsid PubMed
gag Removal of a positively charged residue at position 332 of human TRIM5alpha increases its restricting activity by increasing the ability of TRIM5alpha to bind HIV-1 capsid complexes PubMed
gag Intra- and inter-hexamer crosslinking demonstrate the interaction between HIV-1 CA and human TRIM5alpha CC-SPRY domains (residues 132-493) PubMed
gag HIV-1 CA residues 83, 89, 120, 122 and the aromatic residues 117, 130, and 133 modulate its sensitivity to TRIM5alpha PubMed
gag TRIM5alpha disrupts the regular cylindrical hexameric lattice structure of HIV-1 CA-NC complex PubMed
gag HIV-1 capsid is more rapidly degraded in cells expressing monkey TRIM5alpha than in cells expressing human TRIM5alpha PubMed
gag R332G/R335G and R330E/R332G/R335G mutants of TRIM5alpha binds the in vitro-assembled CA-NC complexes and the strength of the interaction correlates with the magnitude of restriction PubMed
gag Mutation G330E in the v1 TRIM5alpha region exhibits a strong level of HIV-1 resistance, while mutations G330A or G330S have little or no effect PubMed
gag The level of susceptibility hTRIM5alpha expressed by viruses carrying HIV-2 CA sequences in the context of HIV-1 NL4-3 is higher than that of HIV-1 NL4-3 and markedly higher than a panel of primary HIV-1 CA sequences PubMed
gag HIV-1 CA-V86M mutant is partially resistant to restriction by hTRIM5alpha mutant R322G/R335G. The restriction of CA-V86M by hTRIM5alpha R322G/R335G is independent of cyclophilin A, but correlates with its sensitivity to cyclosporine A treatment PubMed
gag TRIM5alpha allelic variants R136Q/H419Y and H43Y/G249D are generally inactive for HIV-1 CA inhibition PubMed
gag The T216I mutation in HIV-1 CA restores the ability of P38A CA mutant particles to saturate TRIM5alpha host restrictions in monkey cells PubMed
gag The TAK1 kinase complex (TAK1/TAB2/TAB3) contributes to capsid-specific restriction mediated by TRIM5alpha. TRIM5alpha activates TAK1 autophosphorylation PubMed
gag Human TRIM5-alpha and TRIM5-delta proteins are dominant negative to HIV-1 restriction in rabbit cells PubMed
gag TRIM5alpha associates with HIV-1 capsid-nucleocapsid complexes assembled in vitro, and the TRIM5alpha interaction with these complexes is dependent on its B30.2(SPRY) domains PubMed
gag Cluster I-A and Cluster II-A changes in the B-box 2 domain of TRIM5alpha decrease its binding to HIV-1 Capsid PubMed
gag Two B-box TRIM5alpha mutants (Cluster I-A:Q109E, E110K, V114A, I115L, and L118V; Cluster II-A:E120A, R121Q, Q123R, and E124K) abolish the weak restricting activity against HIV-1 associated with wild-type TRIM5alpha PubMed
gag Human TRIM5alpha modestly, but specifically, inhibits an HIV-1 strain carrying a mutation (G89V) in the cyclophilin binding loop in capsid PubMed
gag The effect of inhibition of CA-CypA interaction by TRIM5alpha is virus isolates-dependent, which can result in inhibition, no change, or an increase in viral infectivity PubMed
gag TRIM5alpha binds to HIV-1 CA tubular assemblies in a dose-dependent manner, especially at P207/T216 inter-hexamer interfaces PubMed
nucleocapsid gag R332G/R335G and R330E/R332G/R335G mutants of TRIM5alpha binds the in vitro-assembled CA-NC complexes and the strength of the interaction correlates with the magnitude of restriction PubMed
gag TRIM5alpha disrupts the regular cylindrical hexameric lattice structure of HIV-1 CA-NC complex PubMed

Go to the HIV-1, Human Interaction Database

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • AC015691.13

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables pattern recognition receptor activity IDA
Inferred from Direct Assay
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein homodimerization activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables protein homodimerization activity IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein kinase binding IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables protein kinase binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein-macromolecule adaptor activity IPI
Inferred from Physical Interaction
more info
PubMed 
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
PubMed 
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
involved_in activation of innate immune response IDA
Inferred from Direct Assay
more info
PubMed 
involved_in autophagy IDA
Inferred from Direct Assay
more info
PubMed 
involved_in defense response to virus TAS
Traceable Author Statement
more info
PubMed 
involved_in innate immune response IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in innate immune response IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of viral entry into host cell IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of viral release from host cell IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of DNA-binding transcription factor activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of I-kappaB kinase/NF-kappaB signaling IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in positive regulation of I-kappaB kinase/NF-kappaB signaling IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of I-kappaB kinase/NF-kappaB signaling IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of MAPK cascade IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of NF-kappaB transcription factor activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in positive regulation of NF-kappaB transcription factor activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of autophagy IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in positive regulation of transcription, DNA-templated IEA
Inferred from Electronic Annotation
more info
 
involved_in protein K63-linked ubiquitination IDA
Inferred from Direct Assay
more info
PubMed 
involved_in protein polyubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in regulation of gene expression IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in regulation of lipopolysaccharide-mediated signaling pathway IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in regulation of protein localization IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
involved_in regulation of protein localization IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in regulation of viral entry into host cell IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
located_in P-body IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
is_active_in nucleoplasm IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
colocalizes_with omegasome IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
tripartite motif-containing protein 5
Names
RING-type E3 ubiquitin transferase TRIM5
ring finger protein 88
tripartite motif containing 5 transcript variant iota
tripartite motif containing 5 transcript variant kappa
tripartite motif protein TRIM5
NP_149023.2
NP_149083.2
NP_149084.2
XP_005253240.1
XP_005253241.1
XP_006718421.1
XP_011518728.1
XP_011518729.1
XP_016873949.1
XP_016873950.1
XP_016873951.1
XP_016873952.1

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_029122.1 RefSeqGene

    Range
    5036..26915
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_033034.3NP_149023.2  tripartite motif-containing protein 5 isoform alpha

    See identical proteins and their annotated locations for NP_149023.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (alpha) encodes the longest isoform (alpha).
    Source sequence(s)
    AC015691, AK027593, CN334837, DA475412
    Consensus CDS
    CCDS31393.1
    UniProtKB/Swiss-Prot
    Q9C035
    Related
    ENSP00000369373.3, ENST00000380034.8
    Conserved Domains (3) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    cd15822
    Location:288489
    SPRY_PRY_TRIM5; PRY/SPRY domain in tripartite motif-binding protein 5 (TRIM5), also known as RING finger protein 88 (RNF88)
  2. NM_033092.4NP_149083.2  tripartite motif-containing protein 5 isoform gamma

    See identical proteins and their annotated locations for NP_149083.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (gamma) includes an alternate segment in the 3' coding region, compared to variant alpha, which results in a frameshift. The resulting protein (isoform gamma) has a shorter and distinct C-terminus, compared to isoform alpha.
    Source sequence(s)
    AC015691, AF220027, AK074363, BC021258, CN334837, DA475412
    Consensus CDS
    CCDS31394.1
    UniProtKB/Swiss-Prot
    Q9C035
    Related
    ENSP00000380058.3, ENST00000396847.7
    Conserved Domains (4) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    pfam12325
    Location:130175
    TMF_TATA_bd; TATA element modulatory factor 1 TATA binding
    cd16591
    Location:1260
    RING-HC_TRIM5_like-C-IV; RING finger, HC subclass, found in tripartite motif-containing proteins TRIM5, TRIM6, TRIM22, TRIM34 and similar proteins
    cl25732
    Location:116240
    SMC_N; RecF/RecN/SMC N terminal domain
  3. NM_033093.4NP_149084.2  tripartite motif-containing protein 5 isoform delta

    See identical proteins and their annotated locations for NP_149084.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (delta) differs in the 5' UTR and uses an alternate splice site in the 3' terminal exon, which results in a frameshift, compared to variant alpha. The encoded isoform (delta) has a shorter and distinct C-terminus, compared to isoform alpha.
    Source sequence(s)
    AC015691, AC109341, DA475412
    Consensus CDS
    CCDS31392.1
    UniProtKB/Swiss-Prot
    Q9C035
    Related
    ENSP00000369366.1, ENST00000380027.5
    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20210514

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p13 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p13 Primary Assembly

    Range
    5590906..5685108 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017018462.2XP_016873951.1  tripartite motif-containing protein 5 isoform X5

  2. XM_017018461.2XP_016873950.1  tripartite motif-containing protein 5 isoform X4

    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
  3. XM_017018463.2XP_016873952.1  tripartite motif-containing protein 5 isoform X7

    Related
    ENSP00000506742.1, ENST00000682968.1
    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
  4. XM_005253183.3XP_005253240.1  tripartite motif-containing protein 5 isoform X1

    See identical proteins and their annotated locations for XP_005253240.1

    UniProtKB/Swiss-Prot
    Q9C035
    Related
    ENSP00000507420.1, ENST00000684655.1
    Conserved Domains (3) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    cd15822
    Location:288489
    SPRY_PRY_TRIM5; PRY/SPRY domain in tripartite motif-binding protein 5 (TRIM5), also known as RING finger protein 88 (RNF88)
  5. XM_011520426.3XP_011518728.1  tripartite motif-containing protein 5 isoform X4

    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
  6. XM_006718358.3XP_006718421.1  tripartite motif-containing protein 5 isoform X7

    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
  7. XM_005253184.3XP_005253241.1  tripartite motif-containing protein 5 isoform X2

    See identical proteins and their annotated locations for XP_005253241.1

    UniProtKB/Swiss-Prot
    Q9C035
    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
  8. XM_011520427.2XP_011518729.1  tripartite motif-containing protein 5 isoform X6

    See identical proteins and their annotated locations for XP_011518729.1

    UniProtKB/Swiss-Prot
    Q9C035
    Related
    ENSP00000393052.1, ENST00000433961.5
    Conserved Domains (2) summary
    smart00336
    Location:90130
    BBOX; B-Box-type zinc finger
    cd00162
    Location:1459
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
  9. XM_017018460.2XP_016873949.1  tripartite motif-containing protein 5 isoform X3

RNA

  1. XR_001748015.2 RNA Sequence

  2. XR_001748018.2 RNA Sequence

  3. XR_001748014.2 RNA Sequence

  4. XR_001748017.2 RNA Sequence

    Related
    ENST00000684417.1
  5. XR_001748016.2 RNA Sequence

  6. XR_930920.3 RNA Sequence

  7. XR_930921.2 RNA Sequence

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