YUH1 ubiquitin-specific protease YUH1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YUH1
Official Full Name: ubiquitin-specific protease YUH1
Primary source: SGD:S000003860
Locus tag: YJR099W
See related: AllianceGenome:SGD:S000003860; FungiDB:YJR099W; VEuPathDB:YJR099W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables cysteine-type deubiquitinase activity. Predicted to be involved in protein catabolic process. Predicted to act upstream of or within ubiquitin-dependent protein catabolic process. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in Alzheimer's disease; Parkinson's disease; hereditary spastic paraplegia 79A; and hereditary spastic paraplegia 79B. Orthologous to several human genes including UCHL1 (ubiquitin C-terminal hydrolase L1). [provided by Alliance of Genome Resources, Apr 2025]
Orthologs: all
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Genomic context

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See YUH1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (615576..616286)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Following mutagenesis and yeast display screening, this study identified a variant of the yeast ubiquitin C-terminal hydrolase Yuh1 that has a 28-fold improvement in the transamidation to hydrolysis ratio relative to the wild type enzyme. The switch in activity enables robust autoubiquitination of a lysine in the crossover loop to form an isopeptide bond.
Title: Reprogramming a Deubiquitinase into a Transamidase.
Data show that upon complexation of ubiquitin with YUH1, 13C NMR paramagnetic shifts associated with the protein binding interface were reduced by 0.05 ppm or more.
Title: An NMR method for the determination of protein binding interfaces using TEMPOL-induced chemical shift perturbations.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ha3112 (e-PCR)
- UniSTS:515652

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables cysteine-type deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type deubiquitinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cysteine-type deubiquitinase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin recycling	IC Inferred by Curator more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IC Inferred by Curator more info	PubMed

General protein information

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Preferred Names: ubiquitin-specific protease YUH1

NP_012633.1

EC 3.4.19.12

Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

615576..616286

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181757.1 → NP_012633.1 TPA: ubiquitin-specific protease YUH1

See identical proteins and their annotated locations for NP_012633.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWR8, P35127

UniProtKB/TrEMBL

A0A0L8VN61, A6ZQ55, B3LQI4, B5VLP6, C7GMA8, C8ZBP6

Conserved Domains (1) summary

cl08306
Location:8 → 231

Peptidase_C12; Cysteine peptidase C12 contains ubiquitin carboxyl-terminal hydrolase (UCH) families L1, L3, L5 and BAP1