RPA12 DNA-directed RNA polymerase I core subunit RPA12 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPA12
Official Full Name: DNA-directed RNA polymerase I core subunit RPA12
Primary source: SGD:S000003824
Locus tag: YJR063W
See related: AllianceGenome:SGD:S000003824; FungiDB:YJR063W; VEuPathDB:YJR063W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RRN4
Summary: Enables RNA polymerase II-specific DNA-binding transcription factor binding activity. Contributes to DNA-directed RNA polymerase activity. Involved in negative regulation of transcription by RNA polymerase II; nucleolar large rRNA transcription by RNA polymerase I; and termination of RNA polymerase I transcription. Part of RNA polymerase I complex. Orthologous to human POLR1H (RNA polymerase I subunit H). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Genomic context

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See RPA12 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (555195..555572)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RNA polymerase I (Pol I) lobe-binding subunit Rpa12.2 promotes RNA cleavage and proofreading.
Title: RNA polymerase I (Pol I) lobe-binding subunit Rpa12.2 promotes RNA cleavage and proofreading.
Study demonstrate that Rpa12p interacts with the transcription factor Msn4p and prevents its binding to the promoter of AYR1, a key enzyme involved in triacylglycerol biosynthesis and mobilization of nonpolar lipids. These results suggest that the deletion of RPA12 derepresses Msn4p, thus activating the transcription of AYR1, which leads to the accumulation of triacylglycerol in the rpa12D mutant.
Title: The RNA polymerase I subunit Rpa12p interacts with the stress-responsive transcription factor Msn4p to regulate lipid metabolism in budding yeast.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to DNA-directed RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to DNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleolar large rRNA transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleolar large rRNA transcription by RNA polymerase I	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in termination of RNA polymerase I transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in termination of RNA polymerase I transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in termination of RNA polymerase I transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription initiation at RNA polymerase I promoter	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA-directed RNA polymerase I core subunit RPA12

NP_012597.1

RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex; physically interacts with transcriptional activator Msn4p, to regulate transcription of AYR1, a gene involved in lipid metabolism

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

555195..555572

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181721.1 → NP_012597.1 DNA-directed RNA polymerase I core subunit RPA12

See identical proteins and their annotated locations for NP_012597.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWN4, P32529

UniProtKB/TrEMBL

A6ZQ22, B3LQF1, C7GME0, C8ZBL1, G2WH68, N1P1L1

Conserved Domains (1) summary

COG1594
Location:6 → 124

RPB9; DNA-directed RNA polymerase, subunit M/Transcription elongation factor TFIIS [Transcription]