U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

POL31 DNA-directed DNA polymerase delta subunit POL31 [ Saccharomyces cerevisiae S288C ]

Gene ID: 853462, updated on 3-Jun-2026
Official Symbol
POL31
Official Full Name
DNA-directed DNA polymerase delta subunit POL31
Primary source
SGD:S000003766
Locus tag
YJR006W
See related
AllianceGenome:SGD:S000003766; FungiDB:YJR006W; VEuPathDB:YJR006W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
HUS2; HYS2; SDP5
Summary
Enables DNA-directed DNA polymerase activity. Involved in DNA replication, removal of RNA primer and RNA-templated DNA biosynthetic process. Located in cytosol and nucleus. Part of zeta DNA polymerase complex. Used to study cancer. Orthologous to human POLD2 (DNA polymerase delta 2, accessory subunit). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
Try the new Gene page
Try the new Transcripts and proteins table
See POL31 in Genome Data Viewer
Location:
chromosome: X
Exon count:
1
Sequence:
Chromosome: X; NC_001142.9 (449202..450665)

Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene Apl1p Neighboring gene Lso1p Neighboring gene translation initiation factor eIF2 subunit alpha Neighboring gene Mho1p

Genomic Sequence:
NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae. Sugimoto K, et al. Nucleic Acids Res, 1995 Sep 11. PMID 7567461, Free PMC Article
  2. A cancer-associated DNA polymerase delta variant modeled in yeast causes a catastrophic increase in genomic instability. Daee DL, et al. Proc Natl Acad Sci U S A, 2010 Jan 5. PMID 19966286, Free PMC Article
  3. Structure of eukaryotic DNA polymerase δ bound to the PCNA clamp while encircling DNA. Zheng F, et al. Proc Natl Acad Sci U S A, 2020 Dec 1. PMID 33203675, Free PMC Article
  4. DNA polymerase delta, RFC and PCNA are required for repair synthesis of large looped heteroduplexes in Saccharomyces cerevisiae. Corrette-Bennett SE, et al. Nucleic Acids Res, 2004. PMID 15576353, Free PMC Article
  5. The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae. Hashimoto K, et al. Nucleic Acids Res, 1998 Jan 15. PMID 9421503, Free PMC Article

See all (90) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. DNA polymerase delta: A single Pol31 polymorphism suppresses the strain background-specific lethality of Pol32 inactivation in Saccharomyces cerevisiae.
    Title: DNA polymerase δ: A single Pol31 polymorphism suppresses the strain background-specific lethality of Pol32 inactivation in Saccharomyces cerevisiae.
  2. Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.
    Title: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis.
  3. The structure reveals an unexpected arrangement in which the regulatory subunits (Pol31 and Pol32) lie next to the exonuclease domain of Pol3 but do not engage the DNA.
    Title: Cryo-EM structure and dynamics of eukaryotic DNA polymerase δ holoenzyme.
  4. Pol31 and Pol32 subunits of yeast DNA polymerase delta are also essential subunits of DNA polymerase zeta.
    Title: Pol31 and Pol32 subunits of yeast DNA polymerase δ are also essential subunits of DNA polymerase ζ.
  5. Loss of the last four amino acids at the C-terminal end of Pol3 impairs the interaction with Pol31.
    Title: Stable interactions between DNA polymerase δ catalytic and structural subunits are essential for efficient DNA repair.
  6. propose an orientation for the accessory Pol31-Pol32N subunits relative to the Pol3 catalytic core that best agrees with the experimental scattering profile
    Title: Structural insights into yeast DNA polymerase delta by small angle X-ray scattering.
  7. study reveals that Pol31 engages in crosstalk with the other Poldelta subunits, as well as with Mgs1, Rad18 and PCNA; some of the Pol31 regions that are important for such functional cooperations are identified
    Title: Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-directed DNA polymerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-directed DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to DNA-directed DNA polymerase activity TAS
Traceable Author Statement
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to single-stranded DNA 3'-5' DNA exonuclease activity TAS
Traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA damage tolerance TAS
Traceable Author Statement
more info
 PubMed 
involved_in DNA metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication, removal of RNA primer IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA strand elongation involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in RNA-templated DNA biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in base-excision repair TAS
Traceable Author Statement
more info
 PubMed 
involved_in error-prone translesion synthesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lagging strand elongation IEA
Inferred from Electronic Annotation
more info
  
involved_in lagging strand elongation TAS
Traceable Author Statement
more info
 PubMed 
involved_in leading strand elongation TAS
Traceable Author Statement
more info
 PubMed 
involved_in mismatch repair TAS
Traceable Author Statement
more info
 PubMed 
involved_in nucleotide-excision repair TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of delta DNA polymerase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of delta DNA polymerase complex IEA
Inferred from Electronic Annotation
more info
  
part_of delta DNA polymerase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of delta DNA polymerase complex TAS
Traceable Author Statement
more info
 PubMed 
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus NAS
Non-traceable Author Statement
more info
 PubMed 
part_of zeta DNA polymerase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of zeta DNA polymerase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
DNA-directed DNA polymerase delta subunit POL31
NP_012539.1
  • Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001142.9 Reference assembly

    Range
    449202..450665
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181663.1 → NP_012539.1  DNA-directed DNA polymerase delta subunit POL31

    See identical proteins and their annotated locations for NP_012539.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VWI1, P46957
    UniProtKB/TrEMBL
    D3UF73, N1P1G3
    Conserved Domains (1) summary
    COG1311
    Location:5 → 487
    HYS2; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B [Replication, recombination and repair]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P46957.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools
Miscellaneous