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GCD14 tRNA 1-methyladenosine methyltransferase subunit GCD14 [ Saccharomyces cerevisiae S288C ]

Gene ID: 853317, updated on 3-Jun-2026
Official Symbol
GCD14
Official Full Name
tRNA 1-methyladenosine methyltransferase subunit GCD14
Primary source
SGD:S000003661
Locus tag
YJL125C
See related
AllianceGenome:SGD:S000003661; FungiDB:YJL125C; VEuPathDB:YJL125C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
TRM61
Summary
Contributes to tRNA (adenine(58)-N1)-methyltransferase activity. Involved in tRNA methylation. Located in nucleus. Part of tRNA (m1A) methyltransferase complex. Orthologous to human TRMT61A (tRNA methyltransferase 61A). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See GCD14 in Genome Data Viewer
Location:
chromosome: X
Exon count:
1
Sequence:
Chromosome: X; NC_001142.9 (185528..186679, complement)

Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene Spt10p Neighboring gene putative hydrolase Neighboring gene Lsm1p Neighboring gene DUF5427 domain-containing protein MTC1

Genomic Sequence:
NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Crystallization and preliminary X-ray diffraction crystallographic study of tRNA m(1)A58 methyltransferase from Saccharomyces cerevisiae. Qiu X, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2011 Nov 1. PMID 22102254, Free PMC Article
  2. Conserved amino acids in each subunit of the heteroligomeric tRNA m1A58 Mtase from Saccharomyces cerevisiae contribute to tRNA binding. Ozanick SG, et al. Nucleic Acids Res, 2007. PMID 17932071, Free PMC Article
  3. Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 from Saccharomyces cerevisiae. Wang M, et al. Sci Rep, 2016 Sep 1. PMID 27582183, Free PMC Article
  4. The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae. Anderson J, et al. Proc Natl Acad Sci U S A, 2000 May 9. PMID 10779558, Free PMC Article
  5. The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. Anderson J, et al. Genes Dev, 1998 Dec 1. PMID 9851972, Free PMC Article

See all (42) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 has been reported.
    Title: Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 from Saccharomyces cerevisiae.
  2. X-ray diffraction analysis of the TRM6-TRM61 complex were performed in this study. The crystals diffracted to 2.80 A resolution and belonged to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 139.14, c = 101.62 A
    Title: Crystallization and preliminary X-ray diffraction crystallographic study of tRNA m(1)A58 methyltransferase from Saccharomyces cerevisiae.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to tRNA (adenine(58)-N1)-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA (adenine(58)-N1)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to tRNA (adenine(58)-N1)-methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in tRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA methylation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of tRNA (m1A) methyltransferase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of tRNA (m1A) methyltransferase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of tRNA (m1A) methyltransferase complex IEA
Inferred from Electronic Annotation
more info
  
part_of tRNA (m1A) methyltransferase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
tRNA 1-methyladenosine methyltransferase subunit GCD14
NP_012410.1
  • Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001142.9 Reference assembly

    Range
    185528..186679 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181558.1 → NP_012410.1  tRNA 1-methyladenosine methyltransferase subunit GCD14

    See identical proteins and their annotated locations for NP_012410.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VW61, P46959
    UniProtKB/TrEMBL
    G2WGP8, N1P1K0
    Conserved Domains (1) summary
    COG2519
    Location:12 → 291
    Gcd14; tRNA A58 N-methylase Trm61 [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P46959.1

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