TIF2 translation initiation factor eIF4A [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TIF2
Official Full Name: translation initiation factor eIF4A
Primary source: SGD:S000003674
Locus tag: YJL138C
See related: AllianceGenome:SGD:S000003674; FungiDB:YJL138C; VEuPathDB:YJL138C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA helicase activity and translation initiation factor activity. Involved in positive regulation of formation of translation preinitiation complex and translational initiation. Located in cytoplasmic stress granule and plasma membrane. Part of eukaryotic translation initiation factor 4F complex. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with hypotonia and speech delay. Orthologous to human EIF4A1 (eukaryotic translation initiation factor 4A1) and EIF4A2 (eukaryotic translation initiation factor 4A2). [provided by Alliance of Genome Resources, Apr 2025]
Orthologs: all
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Genomic context

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See TIF2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (153504..154691, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
Title: mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.
Findings that eIF4A functionally interacts with the pre-initiation complex and plays important roles beyond unwinding 5'-UTR structure is consistent with a recent proposal that eIF4A modulates the conformation of the 40S ribosomal subunit to promote mRNA recruitment.
Title: Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity.
Findings suggest that Ded1 is critically required to promote scanning through secondary structures within 5' UTRs, and while eIF4A cooperates with Ded1 in this function, it also promotes a step of initiation common to virtually all yeast mRNAs.
Title: Genome-wide analysis of translational efficiency reveals distinct but overlapping functions of yeast DEAD-box RNA helicases Ded1 and eIF4A.
Overexpression of the translation initiation factor TIF2 increases the induction of HXT1 translation.
Title: Expression of the glucose transporter HXT1 involves the Ser-Thr protein phosphatase Sit4 in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on RNA	IDA Inferred from Direct Assay more info	PubMed
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within chromatin looping	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of formation of translation preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within translational initiation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
part_of eukaryotic translation initiation factor 4F complex	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF4A

NP_012397.1

EC 3.6.4.13

Translation initiation factor eIF4A; DEAD-box RNA helicase that couples ATPase activity to mRNA binding and unwinding; ATP hydrolysis accelerates the recruitment of mRNA during 48S preinitiation complex (PIC) assembly; ATPase activity stimulated by either eIF4G1 or by the PIC in an eIF3g and eIFGi subunit-dependent manner, independent of eIF4E and eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E and eIF4G; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

153504..154691 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181571.1 → NP_012397.1 TPA: translation initiation factor eIF4A [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012397.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZQJ1, D6VW46, P10081

UniProtKB/TrEMBL

B3LRD4, C8ZCM2, G2WI87, N1P0H0, N1P0I8

Conserved Domains (1) summary

PTZ00424
Location:10 → 394

PTZ00424; helicase 45; Provisional