CDC6 AAA family ATPase CDC6 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CDC6
Gene description: AAA family ATPase CDC6
Primary source: SGD:S000003730
Locus tag: YJL194W
Gene type: protein coding
RNA name: AAA family ATPase CDC6
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

Genomic context

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See CDC6 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (69338..70879)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Cdc6 degradation triggered by Mck1 kinase was enhanced upon DNA damage.
Title: Cdc6 degradation requires phosphodegron created by GSK-3 and Cdk1 for SCFCdc4 recognition in Saccharomyces cerevisiae.
Cdc6 ATP hydrolysis is therefore required for Cdc6 disengagement from the pre-replicative complex after helicase loading to advance subsequent steps in helicase activation in vivo.
Title: Cdc6 ATPase activity disengages Cdc6 from the pre-replicative complex to promote DNA replication.
Data indicate that Cdt1-MCM2-7 complex activates ORC/Cdc6 ATP-hydrolysis to promote helicase loading.
Title: An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly.
We propose that both CDK and Mck1 kinases are required for Cdc6 degradation to ensure a tight control of DNA replication.
Title: A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication.
Tom1 and the Dia2 control Cdc6 degradation in G1 phase.
Title: The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase.
findings show that Mcm2-7 loading requires the coordinate action of the origin recognition complex and Cdc6 ATPases
Title: Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase.
Cdc6 protein is crucial for DNA replication
Title: Roles of the CDK phosphorylation sites of yeast Cdc6 in chromatin binding and rereplication.
Cdc6p delays a specific subset of mitotic events and that Cdc28 inhibition is not sufficient to account for it. The depletion of Cdc6p in G(2)/M cells reveals that Cdc6p is rate limiting for the degradation of the APC/Cdc20 substrates Pds1p and Clb2p.
Title: Mitotic Cdc6 stabilizes anaphase-promoting complex substrates by a partially Cdc28-independent mechanism, and this stabilization is suppressed by deletion of Cdc55.
Cdc6 and origin DNA activate a molecular switch in Origin Recognition Complex that contributes to pre-RC assembly.
Title: ATPase-dependent cooperative binding of ORC and Cdc6 to origin DNA.
the mitotic CDK, Clb2/Cdc28, binds tightly to an amino-terminal domain of Cdc6, and Cdc6 in this complex is unable to assemble pre-replicative complexes
Title: Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from BioSystems

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Cell Cycle and Cell Division, organism-specific biosystem (from WikiPathways)
Cell Cycle and Cell Division, organism-specific biosystem(From http://en.wikipedia.org/wiki/Cell_cycle) The cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle in...
Cell cycle - yeast, organism-specific biosystem (from KEGG)
Cell cycle - yeast, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
Cell cycle - yeast, conserved biosystem (from KEGG)
Cell cycle - yeast, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
DNA Replication, organism-specific biosystem (from WikiPathways)
DNA Replication, organism-specific biosystemDNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. This process is "replication" in that each strand of the original ...
Meiosis - yeast, organism-specific biosystem (from KEGG)
Meiosis - yeast, organism-specific biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Meiosis - yeast, conserved biosystem (from KEGG)
Meiosis - yeast, conserved biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the CDC6 gene: The CDC6 gene is conserved in S.cerevisiae, K.lactis, E.gossypii, M.oryzae, and N.crassa.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IDA Inferred from Direct Assay more info	PubMed
ATP binding	IEA Inferred from Electronic Annotation more info
ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
ATPase activity	IDA Inferred from Direct Assay more info	PubMed
DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
GTP binding	IDA Inferred from Direct Assay more info	PubMed
GTPase activity	IDA Inferred from Direct Assay more info	PubMed
chromatin binding	IDA Inferred from Direct Assay more info	PubMed
chromatin binding	IMP Inferred from Mutant Phenotype more info	PubMed
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
DNA replication	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IEA Inferred from Electronic Annotation more info
G1/S transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
cell cycle	IEA Inferred from Electronic Annotation more info
cell division	IEA Inferred from Electronic Annotation more info
mitotic DNA replication checkpoint	IBA Inferred from Biological aspect of Ancestor more info	PubMed
mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of DNA-dependent DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of chromatin silencing at telomere	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
chromosome	IEA Inferred from Electronic Annotation more info
cytoplasm	HDA more info	PubMed
nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
nucleus	HDA more info	PubMed
nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: AAA family ATPase CDC6

NP_012341.1

Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

69338..70879

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181627.1 → NP_012341.1 TPA: AAA family ATPase CDC6 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012341.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P09119

Conserved Domains (1) summary

COG1474
Location:77 → 508

CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]