ENO1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ENO1
Primary source: SGD:S000003486
Locus tag: YGR254W
See related: AllianceGenome:SGD:S000003486; FungiDB:YGR254W; VEuPathDB:YGR254W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HSP48
Summary: Enables melatonin binding activity and phosphopyruvate hydratase activity. Involved in glycolytic process and regulation of vacuole fusion, non-autophagic. Located in fungal-type vacuole and mitochondrion. Part of phosphopyruvate hydratase complex. Human ortholog(s) of this gene implicated in Alzheimer's disease and prostate cancer. Orthologous to human ENO1 (enolase 1); ENO2 (enolase 2); and ENO3 (enolase 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (1000927..1002240)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Influence of cytosolic conditions on the reaction equilibrium and the reaction enthalpy of the enolase reaction accessed by calorimetry and van 't HOFF.
Title: Influence of cytosolic conditions on the reaction equilibrium and the reaction enthalpy of the enolase reaction accessed by calorimetry and van 't HOFF.
Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase
Title: Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase.
The mechanism may be important for yeast cells under hypoxia, to quickly provide both energy and substrates for the biosynthesis of lipids and proteins independently of the tricarboxylic acid (TCA) cycle and also to fit changing environments.
Title: Spatial reorganization of Saccharomyces cerevisiae enolase to alter carbon metabolism under hypoxia.
A comparison between in vivo glycated enolase and purified enolase glycated in vitro revealed marked differences.
Title: Protein glycation in vivo: functional and structural effects on yeast enolase.
These results suggest that Ree1p functions in the galactose metabolic pathway via the Gal83 protein and that it may control the level of Eno1p, which is also affected by the Snf1 complex, in S. cerevisiae.
Title: Novel Ree1 regulates the expression of ENO1 via the Snf1 complex pathway in Saccharomyces cerevisiae.
Effects of the G376E and G157D mutations on the stability of yeast enolase
Title: Effects of the G376E and G157D mutations on the stability of yeast enolase--a model for human muscle enolase deficiency.
enolase stimulates vacuole fusion and enolase and Bor1p regulate selective protein trafficking to the vacuole
Title: Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast.
Expression of an ENO1-lacZ reporter was also regulated by the other eight bHLH proteins, namely, Ino2p, Ino4p, Cbf1p, Rtg1p, Rtg3p, Pho4p, Hms1p, and Ygr290wp.
Title: Multiple basic helix-loop-helix proteins regulate expression of the ENO1 gene of Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables melatonin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphopyruvate hydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphopyruvate hydratase activity	IEA Inferred from Electronic Annotation more info
enables phosphopyruvate hydratase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vacuole fusion, non-autophagic	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in fungal-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
part_of phosphopyruvate hydratase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphopyruvate hydratase complex	IDA Inferred from Direct Assay more info	PubMed
part_of phosphopyruvate hydratase complex	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: phosphopyruvate hydratase ENO1

NP_011770.3

EC 4.2.1.11

Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

1000927..1002240

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181383.3 → NP_011770.3 TPA: phosphopyruvate hydratase ENO1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011770.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VV34, P00924, P99013

UniProtKB/TrEMBL

B3LHY6, B5VJJ6, N1P439

Conserved Domains (1) summary

PTZ00081
Location:1 → 430

PTZ00081; enolase; Provisional