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SMI1 Smi1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853144, updated on 24-Jan-2026
Official Symbol
SMI1
Official Full Name
Smi1p
Primary source
SGD:S000003461
Locus tag
YGR229C
See related
AllianceGenome:SGD:S000003461; FungiDB:YGR229C; VEuPathDB:YGR229C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
KNR4
Summary
Predicted to enable DNA binding activity. Involved in regulation of fungal-type cell wall biogenesis and regulation of mitotic cell cycle. Located in cellular bud neck and incipient cellular bud site. [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See SMI1 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (949382..950899, complement)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene dolichyl-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-dolichol alpha-1,2- glucosyltransferase Neighboring gene Oto1p Neighboring gene Bns1p Neighboring gene prohibitin subunit PHB2

Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Analysis of beta-glucans and chitin in a Saccharomyces cerevisiae cell wall mutant using high-performance liquid chromatography. Hong Z, et al. Yeast, 1994 Aug. PMID 7992508
  2. A recombinant Saccharomyces cerevisiae strain overproducing mannoproteins stabilizes wine against protein haze. Gonzalez-Ramos D, et al. Appl Environ Microbiol, 2008 Sep. PMID 18606802, Free PMC Article
  3. Flocculation and transcriptional adaptation to fermentation conditions in a recombinant wine yeast strain defective for KNR4/SMI1. Penacho V, et al. Biotechnol Prog, 2012 Mar-Apr. PMID 22065482
  4. Chemical evaluation of white wines elaborated with a recombinant Saccharomyces cerevisiae strain overproducing mannoproteins. Juega M, et al. Food Chem, 2014 Mar 15. PMID 24206689
  5. Crystallographic studies of the structured core domain of Knr4 from Saccharomyces cerevisiae. Julien S, et al. Acta Crystallogr F Struct Biol Commun, 2015 Sep. PMID 26323295, Free PMC Article

See all (108) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Saccharomyces cerevisiae CellWall Remodeling in the Absence of Knr4 and Kre6 Revealed by Nano-FourierTransform Infrared Spectroscopy.
    Title: Saccharomyces cerevisiae CellWall Remodeling in the Absence of Knr4 and Kre6 Revealed by Nano-FourierTransform Infrared Spectroscopy.
  2. The Conserved Yeast Protein Knr4 Involved in Cell Wall Integrity Is a Multi-domain Intrinsically Disordered Protein.
    Title: The Conserved Yeast Protein Knr4 Involved in Cell Wall Integrity Is a Multi-domain Intrinsically Disordered Protein.
  3. Loss of Smi1, a protein involved in cell wall synthesis, extends replicative life span by enhancing rDNA stability in Saccharomyces cerevisiae.
    Title: Loss of Smi1, a protein involved in cell wall synthesis, extends replicative life span by enhancing rDNA stability in Saccharomyces cerevisiae.
  4. Dysfunctional (mutant) Knr4 protein playing the role of the transcriptional regulator of cell wall chitin and glucan synthesis, the Lifespan increased significantly.
    Title: Cell wall biosynthesis impairment affects the budding lifespan of the Saccharomyces cerevisiae yeast.
  5. Knr4 has been shown to engage in multiple physical interactions, an ability conferred by the intrinsic structural adaptability of major disordered regions present in the N-terminal and C-terminal parts of the protein. [REVIEW]
    Title: Knr4: a disordered hub protein at the heart of fungal cell wall signalling.
  6. crystal structure of structured core domain of Knr4
    Title: Crystallographic studies of the structured core domain of Knr4 from Saccharomyces cerevisiae.
  7. The authors show that the N-terminal domain is required for the proper cellular localization of Knr4 at sites of polarized growth during vegetative growth and sexual differentiation (bud tip and 'shmoo' tip).
    Title: Knr4 N-terminal domain controls its localization and function during sexual differentiation and vegetative growth.
  8. Computational analysis supported by biochemical and biophysical data characterize Knr4 as a newly identified member of the growing family of intrinsically disordered proteins
    Title: Structure-function analysis of Knr4/Smi1, a newly member of intrinsically disordered proteins family, indispensable in the absence of a functional PKC1-SLT2 pathway in Saccharomyces cerevisiae.
  9. Knr4 is a regulatory protein that participates in the coordination of cell wall synthesis with bud emergence, and that this function may be modulated by phosphorylation of this protein
    Title: The 'interactome' of the Knr4/Smi1, a protein implicated in coordinating cell wall synthesis with bud emergence in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables molecular_function ND
No biological Data available
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell wall biogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
involved_in fungal-type cell wall beta-glucan biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of fungal-type cell wall biogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of fungal-type cell wall biogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of mitotic cell cycle IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of mitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cellular bud neck IDA
Inferred from Direct Assay
more info
 PubMed 
located_in incipient cellular bud site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mating projection tip HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
Smi1p
NP_011745.1
  • Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    949382..950899 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181358.1 → NP_011745.1  Smi1p

    See identical proteins and their annotated locations for NP_011745.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VV09, P32566
    UniProtKB/TrEMBL
    B5VJG7, C7GNK0, N1P4C8
    Conserved Domains (2) summary
    PTZ00121
    Location:329 → 501
    PTZ00121; MAEBL; Provisional
    COG4282
    Location:84 → 308
    SMI1; Cell wall assembly regulator SMI1 [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32566.1

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