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STT3 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852862, updated on 19-Nov-2025
Official Symbol
STT3
Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Primary source
SGD:S000002990
Locus tag
YGL022W
See related
AllianceGenome:SGD:S000002990; FungiDB:YGL022W; VEuPathDB:YGL022W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Predicted to enable dolichyl-diphosphooligosaccharide-protein glycotransferase activity. Involved in protein N-linked glycosylation. Located in endoplasmic reticulum. Part of oligosaccharyltransferase complex. Used to study congenital disorder of glycosylation Iw. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Iw and congenital disorder of glycosylation Ix. Orthologous to several human genes including STT3B (STT3 oligosaccharyltransferase complex catalytic subunit B). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
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See STT3 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (452404..454560)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Pgd1p Neighboring gene Pib2p Neighboring gene protein kinase ALK1 Neighboring gene GET complex subunit GET1

Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase. Huang C, et al. J Biol Chem, 2012 Sep 21. PMID 22865878, Free PMC Article
  2. Unraveling the mechanism of protein N-glycosylation. Yan A, et al. J Biol Chem, 2005 Feb 4. PMID 15590627
  3. High-level expression of the Toxoplasma gondii STT3 gene is required for suppression of the yeast STT3 gene mutation. Shams-Eldin H, et al. Mol Biochem Parasitol, 2005 Sep. PMID 15936094
  4. Preferential transfer of the complete glycan is determined by the oligosaccharyltransferase complex and not by the catalytic subunit. Castro O, et al. Proc Natl Acad Sci U S A, 2006 Oct 3. PMID 17001015, Free PMC Article
  5. All in one: Leishmania major STT3 proteins substitute for the whole oligosaccharyltransferase complex in Saccharomyces cerevisiae. Nasab FP, et al. Mol Biol Cell, 2008 Sep. PMID 18596231, Free PMC Article

See all (90) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyl transfer reaction by point mutations in yeast oligosaccharyltransferase.
    Title: Uncoupling the hydrolysis of lipid-linked oligosaccharide from the oligosaccharyl transfer reaction by point mutations in yeast oligosaccharyltransferase.
  2. results show that most of the STT3 TMDs are well inserted into the ER membrane on their own or in the presence of the natural flanking residues.
    Title: Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyltransferase complex.
  3. Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase.
    Title: Eukaryotic N-glycosylation occurs via the membrane-anchored C-terminal domain of the Stt3p subunit of oligosaccharyltransferase.
  4. The cloning, overexpression, purification, and biophysical characterization of the carboxyl-terminal domain of Stt3p is reported; Asp(518) plays a critical structural role, in addition to its previously proposed catalytic role.
    Title: A novel method of production and biophysical characterization of the catalytic domain of yeast oligosaccharyl transferase.
  5. is an essential player in N-glycosylation
    Title: Unraveling the mechanism of protein N-glycosylation.
  6. results suggest that membrane protein STT3 has 11 transmembrane helices and an overall N(cyt)-C(lum) orientation
    Title: Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex.
  7. Homologous Stt3 proteins can serve analogous functions in distantly related eukaryotic cells regardless of their degree of conservation.
    Title: High-level expression of the Toxoplasma gondii STT3 gene is required for suppression of the yeast STT3 gene mutation.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity IC
Inferred by Curator
more info
 PubMed 
enables dolichyl-diphosphooligosaccharide-protein glycotransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oligosaccharyl transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in post-translational protein modification IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein N-linked glycosylation IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in protein N-linked glycosylation via asparagine IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in endoplasmic reticulum HDA PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
part_of oligosaccharyltransferase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of oligosaccharyltransferase complex IEA
Inferred from Electronic Annotation
more info
  
part_of oligosaccharyltransferase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
NP_011493.1
  • Catalytic subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER lumen; involved in recognition of N-acetylglucosamine; forms a subcomplex with Ost3p and Ost4p

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    452404..454560
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180887.1 → NP_011493.1  TPA: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3

    See identical proteins and their annotated locations for NP_011493.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VUB5, P39007
    UniProtKB/TrEMBL
    A6ZUX1, B5VIT9, C7GSA2, G2WE71, N1P3P8
    Conserved Domains (1) summary
    pfam02516
    Location:15 → 475
    STT3; Oligosaccharyl transferase STT3 subunit
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P39007.2

Gene LinkOut

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