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MAD1 coiled-coil domain-containing protein MAD1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852794, updated on 11-May-2025
Official Symbol
MAD1
Official Full Name
coiled-coil domain-containing protein MAD1
Primary source
SGD:S000003054
Locus tag
YGL086W
See related
AllianceGenome:SGD:S000003054; FungiDB:YGL086W; VEuPathDB:YGL086W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in negative regulation of protein import into nucleus during spindle assembly checkpoint; nucleocytoplasmic transport; and regulation of cell cycle phase transition. Located in kinetochore and nucleus. Human ortholog(s) of this gene implicated in lymphoma; mosaic variegated aneuploidy syndrome 7 with inflammation and tumor predisposition; and prostate cancer. Orthologous to human MAD1L1 (mitotic arrest deficient 1 like 1). [provided by Alliance of Genome Resources, May 2025]
Orthologs
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See MAD1 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (347119..349368)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene ncRNA Neighboring gene E2 ubiquitin-conjugating protein MMS2 Neighboring gene Lcl3p Neighboring gene O-acyltransferase

Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast. Hardwick KG, et al. J Cell Biol, 1995 Nov. PMID 7593191, Free PMC Article
  2. The C-terminal half of Saccharomyces cerevisiae Mad1p mediates spindle checkpoint function, chromosome transmission fidelity and CEN association. Kastenmayer JP, et al. Genetics, 2005 Jun. PMID 15802513, Free PMC Article
  3. Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Hardwick KG, et al. Science, 1996 Aug 16. PMID 8688079
  4. Karyotypic determinants of chromosome instability in aneuploid budding yeast. Zhu J, et al. PLoS Genet, 2012. PMID 22615582, Free PMC Article
  5. Dispensability of the SAC Depends on the Time Window Required by Aurora B to Ensure Chromosome Biorientation. Muñoz-Barrera M, et al. PLoS One, 2015. PMID 26661752, Free PMC Article

See all (158) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mad1 recruitment to the kinetochore and it's role in the spindle assembly checkpoint
    Title: Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.
  2. Mad1 is recruited by detached kinetochores competing with alternate other sites in nucleus: Mad1 pool is small enough to be fully depleted by one pair of detached kinetochores. Lateral attachment licenses Mad1 removal from kinetochores after kinetic delay
    Title: Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment.
  3. Mad1p senses unattached kinetochores and, by altering nuclear pore complex transport activity, regulates the nuclear environment of the spindle.
    Title: Mitosis-specific regulation of nuclear transport by the spindle assembly checkpoint protein Mad1p.
  4. cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension.
    Title: Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis.
  5. Only the C terminus is required for spindle assembly checkpoint (SAC) function and the nuclear pore complex, through Nup53p, may act to regulate the duration of the SAC response.
    Title: Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiae.
  6. Saccharomyces cerevisiae Mad1p C-terminal domain mediates spindle checkpoint function, chromosome transmission fidelity and CEN association
    Title: The C-terminal half of Saccharomyces cerevisiae Mad1p mediates spindle checkpoint function, chromosome transmission fidelity and CEN association.
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Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in activation of mitotic cell cycle spindle assembly checkpoint IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in attachment of mitotic spindle microtubules to kinetochore IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic DNA integrity checkpoint signaling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic spindle assembly checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within mitotic spindle assembly checkpoint signaling IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic spindle assembly checkpoint signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of protein import into nucleus during spindle assembly checkpoint IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nucleocytoplasmic transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in nucleocytoplasmic transport IPI
Inferred from Physical Interaction
more info
 PubMed 
Component Evidence Code Pubs
is_active_in kinetochore IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
located_in kinetochore IMP
Inferred from Mutant Phenotype
more info
 PubMed 
is_active_in mitotic spindle IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nuclear envelope IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
coiled-coil domain-containing protein MAD1
NP_011429.3
  • Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    347119..349368
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180951.3 → NP_011429.3  TPA: coiled-coil domain-containing protein MAD1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011429.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VU58, P40957
    UniProtKB/TrEMBL
    B3LHF2, N1P9Q2
    Conserved Domains (1) summary
    pfam05557
    Location:83 → 747
    MAD; Mitotic checkpoint protein
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P40957.1

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