KIP3 tubulin-dependent ATPase KIP3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: KIP3
Gene description: tubulin-dependent ATPase KIP3
Primary source: SGD:S000003184
Locus tag: YGL216W
Gene type: protein coding
RNA name: tubulin-dependent ATPase KIP3
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

Genomic context

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See KIP3 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (84885..87302)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

The single kinesin-8 motor Kip3 alternately mediates both catastrophe and rescue of the bud microtubule (MT). Kip3 accumulates in a length-dependent manner along the lattice of MTs within the bud, yet induces catastrophe spatially near the bud tip.
Title: Spatial control of microtubule length and lifetime by opposing stabilizing and destabilizing functions of Kinesin-8.
Kip3 directly suppresses spindle microtubule polymerization, limiting midzone length.
Title: The kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization.
the motion of yeast (Kip3) and human (Kif18A) kinesin-8s
Title: Kinesin-8 is a low-force motor protein with a weakly bound slip state.
antiparallel microtubule-sliding activity of the budding yeast kinesin-8, Kip3
Title: Microtubule-sliding activity of a kinesin-8 promotes spindle assembly and spindle-length control.
Bud6p and Kip3p play antagonistic roles in controlling the length of microtubules contacting the bud.
Title: Mechanism for astral microtubule capture by cortical Bud6p priming spindle polarity in S. cerevisiae.
Studies indicate that the motor protein Kip3 (in the kinesin-8 family) is an ideal model system to reconstitute high-density traffic on microtubules.
Title: Molecular crowding creates traffic jams of kinesin motors on microtubules.
A model in which TORC1 and Clb5/CDK act coordinately to promote both spindle assembly via a pathway involving Kip3 and S phase progression.
Title: TORC1 kinase and the S-phase cyclin Clb5 collaborate to promote mitotic spindle assembly and DNA replication in S. cerevisiae.
Study found that after binding at a random position on a microtubule and walking to the plus end, an individual Kip3p molecule pauses there until an incoming Kip3p molecule bumps it off.
Title: Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization.
characterization of frictional drag force of KIP3(kinesin-8 motor proteins) interacting with microtubule tracks; argue these frictional forces arise from breaking bonds between motor domains & the microtubule & they limit speed & efficiency of kinesin
Title: Protein friction limits diffusive and directed movements of kinesin motors on microtubules.
Kip3p plays an essential role in positioning the mitotic spindle in budding yeast and potentially other processes controlled by kinesin-8 family members.
Title: Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from BioSystems

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COPI-dependent Golgi-to-ER retrograde traffic, organism-specific biosystem (from REACTOME)
COPI-dependent Golgi-to-ER retrograde traffic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Factors involved in megakaryocyte development and platelet production, organism-specific biosystem (from REACTOME)
Factors involved in megakaryocyte development and platelet production, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Golgi-to-ER retrograde transport, organism-specific biosystem (from REACTOME)
Golgi-to-ER retrograde transport, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Hedgehog 'off' state, organism-specific biosystem (from REACTOME)
Hedgehog 'off' state, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Hemostasis, organism-specific biosystem (from REACTOME)
Hemostasis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Intra-Golgi and retrograde Golgi-to-ER traffic, organism-specific biosystem (from REACTOME)
Intra-Golgi and retrograde Golgi-to-ER traffic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Kinesins, organism-specific biosystem (from REACTOME)
Kinesins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Membrane Trafficking, organism-specific biosystem (from REACTOME)
Membrane Trafficking, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signal Transduction, organism-specific biosystem (from REACTOME)
Signal Transduction, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signaling by Hedgehog, organism-specific biosystem (from REACTOME)
Signaling by Hedgehog, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Vesicle-mediated transport, organism-specific biosystem (from REACTOME)
Vesicle-mediated transport, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the KIP3 gene: The KIP3 gene is conserved in S.cerevisiae, K.lactis, and E.gossypii.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
ATP-dependent microtubule motor activity, plus-end-directed	IBA Inferred from Biological aspect of Ancestor more info	PubMed
ATP-dependent microtubule motor activity, plus-end-directed	IDA Inferred from Direct Assay more info	PubMed
ATPase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule binding	IEA Inferred from Electronic Annotation more info
microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule motor activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
tubulin-dependent ATPase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
establishment of mitotic spindle orientation	IGI Inferred from Genetic Interaction more info	PubMed
establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
microtubule depolymerization	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule-based movement	IEA Inferred from Electronic Annotation more info
mitotic sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
mitotic spindle disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
mitotic spindle organization	IGI Inferred from Genetic Interaction more info	PubMed
mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of microtubule polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
nuclear migration along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
plus-end specific microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
regulation of mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
cytoskeleton	IEA Inferred from Electronic Annotation more info
kinesin complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
kinesin complex	TAS Traceable Author Statement more info	PubMed
microtubule	IBA Inferred from Biological aspect of Ancestor more info	PubMed
microtubule	IEA Inferred from Electronic Annotation more info
mitotic spindle astral microtubule	IBA Inferred from Biological aspect of Ancestor more info	PubMed
mitotic spindle midzone	IBA Inferred from Biological aspect of Ancestor more info	PubMed
nuclear microtubule	IDA Inferred from Direct Assay more info	PubMed
nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed

General protein information

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Preferred Names: tubulin-dependent ATPase KIP3

NP_011299.1

Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

84885..87302

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181081.1 → NP_011299.1 TPA: tubulin-dependent ATPase KIP3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011299.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P53086

Conserved Domains (2) summary

COG5059
Location:85 → 653

KIP1; Kinesin-like protein [Cytoskeleton]

cd01370
Location:82 → 438

KISc_KIP3_like; Kinesin motor domain, KIP3-like subgroup