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DUT1 bifunctional dITP/dUTP diphosphatase [ Saccharomyces cerevisiae S288C ]

Gene ID: 852554, updated on 11-Jun-2025
Official Symbol
DUT1
Official Full Name
bifunctional dITP/dUTP diphosphatase
Primary source
SGD:S000000456
Locus tag
YBR252W
See related
AllianceGenome:SGD:S000000456; FungiDB:YBR252W; VEuPathDB:YBR252W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables dITP diphosphatase activity and dUTP diphosphatase activity. Involved in dITP catabolic process and dUTP catabolic process. Located in cytoplasm and nucleus. Used to study cancer. Orthologous to human DUT (deoxyuridine triphosphatase). [provided by Alliance of Genome Resources, Jun 2025]
Orthologs
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See DUT1 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (722611..723054)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Spo23p Neighboring gene mitochondrial 37S ribosomal protein MRPS5 Neighboring gene Srb6p Neighboring gene TRAPP subunit TRS20

Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The complete sequence of a 6794 bp segment located on the right arm of chromosome II of Saccharomyces cerevisiae. Finding of a putative dUTPase in a yeast. Doignon F, et al. Yeast, 1993 Oct. PMID 8256522
  2. Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme. Tchigvintsev A, et al. Biochem J, 2011 Jul 15. PMID 21548881, Free PMC Article
  3. dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae. Gadsden MH, et al. EMBO J, 1993 Nov. PMID 8223452, Free PMC Article
  4. Homologous recombination is stimulated by a decrease in dUTPase in Arabidopsis. Dubois E, et al. PLoS One, 2011 Apr 26. PMID 21541310, Free PMC Article
  5. Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae. McIntosh EM, et al. Mol Gen Genet, 1986 Sep. PMID 3020375

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The structure of DUT1 and site-directed mutagenesis support a role of the conserved Phe142 in the interaction with the uracil base.
    Title: Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme.
  2. Dut1 plays a central role in the prevention of the formation of endogenous DNA damage.
    Title: dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables dITP diphosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dUTP diphosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dUTP diphosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dUTP diphosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables dUTP diphosphatase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in dITP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dUMP biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in dUMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in dUTP catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in dUTP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dUTP catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in dUTP catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nucleotide metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in pyrimidine deoxyribonucleoside triphosphate catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in nucleus HDA PubMed 
Preferred Names
bifunctional dITP/dUTP diphosphatase
NP_009811.3
  • Deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate; human homolog DUT allows growth of yeast haploid dut1 null mutant after sporulation of heterozygous diploid

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    722611..723054
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178600.3 → NP_009811.3  TPA: bifunctional dITP/dUTP diphosphatase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009811.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQP8, P33317
    UniProtKB/TrEMBL
    A6ZLJ1, B3LMR0, C7GMX9, D3UEZ9, G2W9Q5, N1P8T1
    Conserved Domains (1) summary
    TIGR00576
    Location:9 → 146
    dut; deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P33317.2

Gene LinkOut

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