CDC28 cyclin-dependent serine/threonine-protein kinase CDC28 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CDC28
Gene description: cyclin-dependent serine/threonine-protein kinase CDC28
Primary source: SGD:S000000364
Locus tag: YBR160W
See related: AllianceGenome:SGD:S000000364
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDK1; HSL5; SRM5
Summary: Enables RNA polymerase II complex binding activity; cyclin-dependent protein serine/threonine kinase activity; and histone binding activity. Involved in several processes, including chromosome organization; regulation of cell cycle process; and regulation of nucleobase-containing compound metabolic process. Located in cellular bud neck; endoplasmic reticulum; and nucleus. Part of cyclin-dependent protein kinase holoenzyme complex. Used to study cancer. Human ortholog(s) of this gene implicated in breast cancer; cervical cancer; prostate cancer; renal cell carcinoma; and urinary bladder cancer. Orthologous to several human genes including CDK1 (cyclin dependent kinase 1) and CDK2 (cyclin dependent kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CDC28 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (560078..560974)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Multisite phosphorylation by Cdk1 initiates delayed negative feedback to control mitotic transcription.
Title: Multisite phosphorylation by Cdk1 initiates delayed negative feedback to control mitotic transcription.
Compensation for the absence of the catalytically active half of DNA polymerase epsilon in yeast by positively selected mutations in CDC28.
Title: Compensation for the absence of the catalytically active half of DNA polymerase ε in yeast by positively selected mutations in CDC28.
Cdc42-Specific GTPase-Activating Protein Rga1 Squelches Crosstalk between the High-Osmolarity Glycerol (HOG) and Mating Pheromone Response MAPK Pathways.
Title: Cdc42-Specific GTPase-Activating Protein Rga1 Squelches Crosstalk between the High-Osmolarity Glycerol (HOG) and Mating Pheromone Response MAPK Pathways.
G1 cyclin-Cdk promotes cell cycle entry through localized phosphorylation of RNA polymerase II.
Title: G(1) cyclin-Cdk promotes cell cycle entry through localized phosphorylation of RNA polymerase II.
The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis.
Title: The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis.
Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Title: Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Findings indicate that under optimal growth conditions Cdc28 cyclin dependent kinase Cdk1 (also known as Cdc28) gates tRNA synthesis in S phase by regulating the RNAPIII machinery.
Title: Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity.
Moreover, the authors show that high levels of histones promote Swe1(WEE1) stabilisation thereby triggering the phosphorylation and inhibition of Cdc28(CDK1) through a mechanism different of the canonical DNA damage response.
Title: High levels of histones promote whole-genome-duplications and trigger a Swe1(WEE1)-dependent phosphorylation of Cdc28(CDK1).
Rnr2-Rnr4 redistribution in S phase is triggered by Clb6-Cdc28-mediated phosphorylation of Rnr2, which disrupts the Rnr2-Wtm1 interaction and promotes the release of Rnr2-Rnr4 from the nucleus.
Title: Clb6-Cdc28 Promotes Ribonucleotide Reductase Subcellular Redistribution during S Phase.
Cdc28/CDK1 modulates Rad5 stability by phosphorylation during the cell cycle, suggesting a crosstalk between the phosphorylation and degradation of Rad5.
Title: Cyclin-dependent kinase modulates budding yeast Rad5 stability during cell cycle.

Submit: New GeneRIF Correction See all GeneRIFs (92)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the CDC28 gene: The CDC28 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II complex binding	IDA Inferred from Direct Assay more info	PubMed
enables cyclin binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables cyclin-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables cyclin-dependent protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cyclin-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables histone binding	IDA Inferred from Direct Assay more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 7-methylguanosine mRNA capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G1/S transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid biorientation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of DNA replication origin binding	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA-templated transcription	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of glycogen catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of meiotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic DNA damage checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic spindle pole body separation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of mitotic spindle pole body separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of nuclear cell cycle DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of trehalose catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of trehalose catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of triglyceride catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of triglyceride catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-Golgi vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to nuclear periphery	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to spindle microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of G2/M transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in regulation of budding cell apical bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of budding cell apical bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization to chromatin	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of telomere maintenance via telomerase	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to organic substance	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
part_of cyclin-dependent protein kinase holoenzyme complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
part_of cyclin-dependent protein kinase holoenzyme complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cyclin-dependent serine/threonine-protein kinase CDC28

NP_009718.3

EC 2.7.11.22

Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

560078..560974

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178508.3 → NP_009718.3 TPA: cyclin-dependent serine/threonine-protein kinase CDC28 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009718.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQF5, P00546

UniProtKB/TrEMBL

A0A0L8VVJ6, A0A6A5Q506, A0A6C1DM40, A6ZLA2, B3LMZ9, C7GUU4, D3UEQ4, G2W9G2, H0GCM2, N1P6U1

Conserved Domains (1) summary

cd07835
Location:8 → 295

STKc_CDK1_CdkB_like; Catalytic domain of Cyclin-Dependent protein Kinase 1-like Serine/Threonine Kinases and of Plant B-type Cyclin-Dependent protein Kinase