RPB5 DNA-directed RNA polymerase core subunit RPB5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPB5
Official Full Name: DNA-directed RNA polymerase core subunit RPB5
Primary source: SGD:S000000358
Locus tag: YBR154C
See related: AllianceGenome:SGD:S000000358; FungiDB:YBR154C; VEuPathDB:YBR154C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to DNA-directed RNA polymerase activity and RNA-directed RNA polymerase activity. Involved in tRNA transcription by RNA polymerase III; transcription by RNA polymerase I; and transcription by RNA polymerase II. Part of RNA polymerase I complex; RNA polymerase II, core complex; and RNA polymerase III complex. Orthologous to human POLR2E (RNA polymerase II, I and III subunit E). [provided by Alliance of Genome Resources, Jun 2025]
Orthologs: all
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Genomic context

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See RPB5 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (548362..549009, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

All together, our results reveal an important role of Rpb5 in the transition from initiation to elongation mediated by the RNA polymerase II, by modulating the Spt5 association, and the backtracking activity of the enzyme.
Title: Rpb5 modulates the RNA polymerase II transition from initiation to elongation by influencing Spt5 association and backtracking.
Our data also suggest that the role of Bud27 Rpb5-binding but not PFD-binding domain is necessary for RNA polymerases biogenesis
Title: The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner.
Data show that Top2 is required redundantly with Top1 for optimal recruitment of RNA polymerase II at their promoters.
Title: Topoisomerase II binds nucleosome-free DNA and acts redundantly with topoisomerase I to enhance recruitment of RNA Pol II in budding yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA-directed RNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to DNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed RNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA-directed RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in tRNA transcription by RNA polymerase III	IBA Inferred from Biological aspect of Ancestor more info
involved_in tRNA transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase III	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase III	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription elongation by RNA polymerase I	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription elongation by RNA polymerase I	IEA Inferred from Electronic Annotation more info
involved_in transcription initiation at RNA polymerase II promoter	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase I complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase II, core complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase III complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase III complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase III complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase core subunit RPB5

NP_009712.1

RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

548362..549009 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178502.1 → NP_009712.1 TPA: DNA-directed RNA polymerase core subunit RPB5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009712.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQE9, P20434, Q02121

UniProtKB/TrEMBL

A6ZL96, B3LN05, B5VEB1, C7GUV0, D3UEP8, G2W9F6, N1P8J6

Conserved Domains (1) summary

cl29798
Location:6 → 215

RNA_pol_Rpb5_N; RNA polymerase Rpb5, N-terminal domain