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POL30 proliferating cell nuclear antigen [ Saccharomyces cerevisiae S288C ]

Gene ID: 852385, updated on 3-Dec-2023

Summary

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Gene symbol
POL30
Gene description
proliferating cell nuclear antigen
Primary source
SGD:S000000292
Locus tag
YBR088C
See related
AllianceGenome:SGD:S000000292
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables DNA polymerase processivity factor activity. Involved in DNA metabolic process; chromosome organization; and positive regulation of hydrolase activity. Located in nucleus and replication fork. Part of PCNA complex. Human ortholog(s) of this gene implicated in stomach cancer. Orthologous to human PCNA (proliferating cell nuclear antigen). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See POL30 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (424990..425766, complement)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Ist2p Neighboring gene replication factor C subunit 5 Neighboring gene uncharacterized protein Neighboring gene high-mobility group nucleosome-binding protein Neighboring gene Tim12p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. DNA-damage tolerance through PCNA ubiquitination and sumoylation. Fan L, et al. Biochem J, 2020 Jul 31. PMID 32726436
  2. Crystal structures of PCNA mutant proteins defective in gene silencing suggest a novel interaction site on the front face of the PCNA ring. Kondratick CM, et al. PLoS One, 2018. PMID 29499038, Free PMC Article
  3. Proliferating cell nuclear antigen (PCNA) contributes to the high-order structure and stability of heterochromatin in Saccharomyces cerevisiae. Bi X, et al. Chromosome Res, 2017 Jun. PMID 27987109
  4. Conformational analysis of processivity clamps in solution demonstrates that tertiary structure does not correlate with protein dynamics. Fang J, et al. Structure, 2014 Apr 8. PMID 24613485, Free PMC Article
  5. Probing DNA clamps with single-molecule force spectroscopy. Wang L, et al. Nucleic Acids Res, 2013 Sep. PMID 23783571, Free PMC Article

See all (344) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
    Title: Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
  2. PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.
    Title: PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.
  3. Photo-activatable Ub-PCNA probes reveal new structural features of the Saccharomyces cerevisiae Poleta/PCNA complex.
    Title: Photo-activatable Ub-PCNA probes reveal new structural features of the Saccharomyces cerevisiae Polη/PCNA complex.
  4. Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.
    Title: Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.
  5. Access to PCNA by Srs2 and Elg1 Controls the Choice between Alternative Repair Pathways in Saccharomyces cerevisiae.
    Title: Access to PCNA by Srs2 and Elg1 Controls the Choice between Alternative Repair Pathways in Saccharomyces cerevisiae.
  6. DNA-damage tolerance through PCNA ubiquitination and sumoylation.
    Title: DNA-damage tolerance through PCNA ubiquitination and sumoylation.
  7. the silencing-loss phenotypes of pol30-6 and pol30-79 depended on ploidy, but not on POL30 dosage or mating-type identity. Separately from silencing loss, the pol30-6 and pol30-79 alleles also displayed high levels of mitotic recombination in diploids. These results established that histone trafficking involving PCNA at replication forks is crucial to the maintenance of chromatin state and genome stability during DNA repl
    Title: Mutations in the PCNA DNA Polymerase Clamp of Saccharomyces cerevisiae Reveal Complexities of the Cell Cycle and Ploidy on Heterochromatin Assembly.
  8. PCNA retention controlled by the Elg1 complex is critical for efficient MMR: PCNA needs to be on DNA long enough to enable MMR, but if it is retained too long it interferes with downstream repair steps.
    Title: Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex.
  9. proliferating cell nuclear antigen (PCNA) unloading activity of Elg1 is important for coordinating DNA replication forks with the process of replication-coupled nucleosome assembly to maintain silencing of HML and HMR through S-phase
    Title: Pivotal roles of PCNA loading and unloading in heterochromatin function.
  10. Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
    Title: Role of PCNA and RFC in promoting Mus81-complex activity.
Submit: New GeneRIF Correction See all GeneRIFs (48)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA polymerase processivity factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA polymerase processivity factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA polymerase processivity factor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in error-free translesion synthesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in establishment of mitotic sister chromatid cohesion IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in lagging strand elongation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lagging strand elongation IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in leading strand elongation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in leading strand elongation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in maintenance of DNA trinucleotide repeats IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in maintenance of DNA trinucleotide repeats IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in meiotic mismatch repair IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in meiotic mismatch repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mismatch repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mismatch repair IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mismatch repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mismatch repair IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in mitotic cell cycle IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic sister chromatid cohesion IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic sister chromatid cohesion IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in nucleotide-excision repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of DNA metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in postreplication repair IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in postreplication repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in silent mating-type cassette heterochromatin formation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in silent mating-type cassette heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in subtelomeric heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in translesion synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of PCNA complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of PCNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in chromosome, telomeric region IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in replication fork IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
proliferating cell nuclear antigen
NP_009645.1
  • Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase delta; may function as docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays crucial role during Okazaki fragment processing; Leu164 is essential for stable complex formation with replicative DNA polymerase delta

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    424990..425766 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178436.1 → NP_009645.1  TPA: proliferating cell nuclear antigen [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009645.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQ89, P15873
    UniProtKB/TrEMBL
    A0A6B7JGY6, A6ZL36, B3LN62, C7GJS1, D3UEI3, G2W996, N1P843
    Conserved Domains (1) summary
    TIGR00590
    Location:1 → 258
    pcna; proliferating cell nuclear antigen (pcna)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P15873.1

Gene LinkOut

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