POL30 proliferating cell nuclear antigen [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: POL30
Gene description: proliferating cell nuclear antigen
Primary source: SGD:S000000292
Locus tag: YBR088C
Gene type: protein coding
RNA name: proliferating cell nuclear antigen
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces

Genomic context

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See POL30 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (424990..425766, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

proliferating cell nuclear antigen (PCNA) unloading activity of Elg1 is important for coordinating DNA replication forks with the process of replication-coupled nucleosome assembly to maintain silencing of HML and HMR through S-phase
Title: Pivotal roles of PCNA loading and unloading in heterochromatin function.
Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
Title: Role of PCNA and RFC in promoting Mus81-complex activity.
found evidence implicating PCNA in the maintenance of the high-order structure and stability of heterochromatin, which indicates a role of DNA replication in heterochromatin maintenance
Title: Proliferating cell nuclear antigen (PCNA) contributes to the high-order structure and stability of heterochromatin in Saccharomyces cerevisiae.
Eco1 mediated acetylation regulates PCNA sliding on DNA in the presence of DNA damage, favoring homologous recombination linked to sister-chromatid cohesion.
Title: Acetylation of PCNA Sliding Surface by Eco1 Promotes Genome Stability through Homologous Recombination.
Srs2 interaction with PCNA allows the helicase activity to unwind fork-blocking CAG/CTG hairpin structures to prevent DNA breaks during DNA replication. Independently of PCNA binding, Srs2 also displaces Rad51 from nascent strands to prevent recombination-dependent repeat expansions and contractions.
Title: Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats.
the various roles of Elg1 in genome stability maintenance involve the unloading of both modified and unmodified PCNA.
Title: A structure-function analysis of the yeast Elg1 protein reveals the importance of PCNA unloading in genome stability maintenance.
The Pol30-K196 residue plays a critical role in budding yeast DNA post-replication repair through interaction with Rad18.
Title: The Pol30-K196 residue plays a critical role in budding yeast DNA postreplication repair through interaction with Rad18.
Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction.
Title: Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction.
results suggest that Elg1-RLC acts as a general PCNA unloader and is dependent upon DNA ligation during chromosome replication.
Title: Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment Ligation.
Elevated levels of PCNA rescue pds5-1 temperature sensitivity and cohesion defects, but do not rescue pds5-1 mutant cell condensation defects.
Title: Pds5 regulators segregate cohesion and condensation pathways in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (40)

Pathways from BioSystems

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Base Excision Repair, organism-specific biosystem (from REACTOME)
Base Excision Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Base excision repair, organism-specific biosystem (from KEGG)
Base excision repair, organism-specific biosystemBase excision repair (BER) is the predominant DNA damage repair pathway for the processing of small base lesions, derived from oxidation and alkylation damages. BER is normally defined as DNA repair ...
Base excision repair, conserved biosystem (from KEGG)
Base excision repair, conserved biosystemBase excision repair (BER) is the predominant DNA damage repair pathway for the processing of small base lesions, derived from oxidation and alkylation damages. BER is normally defined as DNA repair ...
Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
Cell Cycle, Mitotic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Chromosome Maintenance, organism-specific biosystem (from REACTOME)
Chromosome Maintenance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA Damage Bypass, organism-specific biosystem (from REACTOME)
DNA Damage Bypass, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA Repair, organism-specific biosystem (from REACTOME)
DNA Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA Replication, organism-specific biosystem (from WikiPathways)
DNA Replication, organism-specific biosystemDNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. This process is "replication" in that each strand of the original ...
DNA Replication, organism-specific biosystem (from REACTOME)
DNA Replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA replication, organism-specific biosystem (from KEGG)
DNA replication, organism-specific biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA replication, conserved biosystem (from KEGG)
DNA replication, conserved biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA strand elongation, organism-specific biosystem (from REACTOME)
DNA strand elongation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Dual Incision in GG-NER, organism-specific biosystem (from REACTOME)
Dual Incision in GG-NER, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Dual incision in TC-NER, organism-specific biosystem (from REACTOME)
Dual incision in TC-NER, organism-specific biosystemcomputationally inferred pathway (not manually curated)
E2F mediated regulation of DNA replication, organism-specific biosystem (from REACTOME)
E2F mediated regulation of DNA replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Extension of Telomeres, organism-specific biosystem (from REACTOME)
Extension of Telomeres, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G1/S Transition, organism-specific biosystem (from REACTOME)
G1/S Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G1/S-Specific Transcription, organism-specific biosystem (from REACTOME)
G1/S-Specific Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Gap-filling DNA repair synthesis and ligation in GG-NER, organism-specific biosystem (from REACTOME)
Gap-filling DNA repair synthesis and ligation in GG-NER, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Gap-filling DNA repair synthesis and ligation in TC-NER, organism-specific biosystem (from REACTOME)
Gap-filling DNA repair synthesis and ligation in TC-NER, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Global Genome Nucleotide Excision Repair (GG-NER), organism-specific biosystem (from REACTOME)
Global Genome Nucleotide Excision Repair (GG-NER), organism-specific biosystemcomputationally inferred pathway (not manually curated)
Lagging Strand Synthesis, organism-specific biosystem (from REACTOME)
Lagging Strand Synthesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Leading Strand Synthesis, organism-specific biosystem (from REACTOME)
Leading Strand Synthesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of proteins, organism-specific biosystem (from REACTOME)
Metabolism of proteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Mismatch Repair, organism-specific biosystem (from REACTOME)
Mismatch Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Mismatch repair, organism-specific biosystem (from KEGG)
Mismatch repair, organism-specific biosystemDNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preven...
Mismatch repair, conserved biosystem (from KEGG)
Mismatch repair, conserved biosystemDNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preven...
Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha), organism-specific biosystem (from REACTOME)
Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha), organism-specific biosystemcomputationally inferred pathway (not manually curated)
Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
Mitotic G1-G1/S phases, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Nucleotide Excision Repair, organism-specific biosystem (from REACTOME)
Nucleotide Excision Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Nucleotide excision repair, organism-specific biosystem (from KEGG)
Nucleotide excision repair, organism-specific biosystemNucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the ...
Nucleotide excision repair, conserved biosystem (from KEGG)
Nucleotide excision repair, conserved biosystemNucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the ...
PCNA-Dependent Long Patch Base Excision Repair, organism-specific biosystem (from REACTOME)
PCNA-Dependent Long Patch Base Excision Repair, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Polymerase switching, organism-specific biosystem (from REACTOME)
Polymerase switching, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Polymerase switching on the C-strand of the telomere, organism-specific biosystem (from REACTOME)
Polymerase switching on the C-strand of the telomere, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Post-translational protein modification, organism-specific biosystem (from REACTOME)
Post-translational protein modification, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Processive synthesis on the lagging strand, organism-specific biosystem (from REACTOME)
Processive synthesis on the lagging strand, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Recognition of DNA damage by PCNA-containing replication complex, organism-specific biosystem (from REACTOME)
Recognition of DNA damage by PCNA-containing replication complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Removal of the Flap Intermediate, organism-specific biosystem (from REACTOME)
Removal of the Flap Intermediate, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Resolution of AP sites via the multiple-nucleotide patch replacement pathway, organism-specific biosystem (from REACTOME)
Resolution of AP sites via the multiple-nucleotide patch replacement pathway, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Resolution of Abasic Sites (AP sites), organism-specific biosystem (from REACTOME)
Resolution of Abasic Sites (AP sites), organism-specific biosystemcomputationally inferred pathway (not manually curated)
S Phase, organism-specific biosystem (from REACTOME)
S Phase, organism-specific biosystemcomputationally inferred pathway (not manually curated)
SUMO E3 ligases SUMOylate target proteins, organism-specific biosystem (from REACTOME)
SUMO E3 ligases SUMOylate target proteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
SUMOylation, organism-specific biosystem (from REACTOME)
SUMOylation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
SUMOylation of DNA replication proteins, organism-specific biosystem (from REACTOME)
SUMOylation of DNA replication proteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Synthesis of DNA, organism-specific biosystem (from REACTOME)
Synthesis of DNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Telomere C-strand (Lagging Strand) Synthesis, organism-specific biosystem (from REACTOME)
Telomere C-strand (Lagging Strand) Synthesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Telomere Maintenance, organism-specific biosystem (from REACTOME)
Telomere Maintenance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Termination of translesion DNA synthesis, organism-specific biosystem (from REACTOME)
Termination of translesion DNA synthesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Transcription-Coupled Nucleotide Excision Repair (TC-NER), organism-specific biosystem (from REACTOME)
Transcription-Coupled Nucleotide Excision Repair (TC-NER), organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translesion Synthesis by POLH, organism-specific biosystem (from REACTOME)
Translesion Synthesis by POLH, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translesion synthesis by POLI, organism-specific biosystem (from REACTOME)
Translesion synthesis by POLI, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translesion synthesis by POLK, organism-specific biosystem (from REACTOME)
Translesion synthesis by POLK, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translesion synthesis by REV1, organism-specific biosystem (from REACTOME)
Translesion synthesis by REV1, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template, organism-specific biosystem (from REACTOME)
Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the POL30 gene: The POL30 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
DNA polymerase processivity factor activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA polymerase processivity factor activity	IDA Inferred from Direct Assay more info	PubMed
DNA polymerase processivity factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
DNA repair	IEA Inferred from Electronic Annotation more info
DNA replication	IEA Inferred from Electronic Annotation more info
cellular response to DNA damage stimulus	IEA Inferred from Electronic Annotation more info
chromatin silencing at silent mating-type cassette	IGI Inferred from Genetic Interaction more info	PubMed
chromatin silencing at silent mating-type cassette	IMP Inferred from Mutant Phenotype more info	PubMed
chromatin silencing at telomere	IMP Inferred from Mutant Phenotype more info	PubMed
error-free translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
establishment of mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
lagging strand elongation	IDA Inferred from Direct Assay more info	PubMed
lagging strand elongation	IPI Inferred from Physical Interaction more info	PubMed
leading strand elongation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
leading strand elongation	IDA Inferred from Direct Assay more info	PubMed
maintenance of DNA trinucleotide repeats	IGI Inferred from Genetic Interaction more info	PubMed
maintenance of DNA trinucleotide repeats	IMP Inferred from Mutant Phenotype more info	PubMed
meiotic mismatch repair	IGI Inferred from Genetic Interaction more info	PubMed
meiotic mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
mismatch repair	IBA Inferred from Biological aspect of Ancestor more info	PubMed
mismatch repair	IGI Inferred from Genetic Interaction more info	PubMed
mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
mismatch repair	IPI Inferred from Physical Interaction more info	PubMed
mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
mitotic sister chromatid cohesion	IPI Inferred from Physical Interaction more info	PubMed
nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of exodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
positive regulation of phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands	IDA Inferred from Direct Assay more info	PubMed
postreplication repair	IGI Inferred from Genetic Interaction more info	PubMed
postreplication repair	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of DNA replication	IEA Inferred from Electronic Annotation more info
translesion synthesis	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Component	Evidence Code	Pubs
PCNA complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
PCNA complex	IPI Inferred from Physical Interaction more info	PubMed
chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	IEA Inferred from Electronic Annotation more info
replication fork	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proliferating cell nuclear antigen

NP_009645.1

Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays a crucial role during Okazaki fragment processing

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

424990..425766 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178436.1 → NP_009645.1 TPA: proliferating cell nuclear antigen [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009645.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P15873

Conserved Domains (1) summary

TIGR00590
Location:1 → 258

pcna; proliferating cell nuclear antigen (pcna)