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PHO8 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852092, updated on 11-Sep-2024

Summary

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Official Symbol
PHO8
Primary source
SGD:S000002889
Locus tag
YDR481C
See related
AllianceGenome:SGD:S000002889; FungiDB:YDR481C; VEuPathDB:YDR481C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables alkaline phosphatase activity. Involved in nicotinamide nucleotide metabolic process and protein dephosphorylation. Located in fungal-type vacuole membrane. Used to study several diseases, including bone disease (multiple); cryptorchidism; germ cell cancer (multiple); hypophosphatasia (multiple); and primary biliary cholangitis. Human ortholog(s) of this gene implicated in adult hypophosphatasia; childhood hypophosphatasia; hypophosphatasia; infantile hypophosphatasia; and inflammatory bowel disease. Orthologous to several human genes including ALPL (alkaline phosphatase, biomineralization associated) and ALPP (alkaline phosphatase, placental). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (1418550..1420250, complement)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Pex29p Neighboring gene Dig2p Neighboring gene U2-type spliceosomal complex subunit CWC21 Neighboring gene alpha-1,2-mannosyltransferase KRE2

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. PHO8 gene coding alkaline phosphatase of Saccharomyces cerevisiae is involved in polyphosphate metabolism. Kizawa K, et al. J Gen Appl Microbiol, 2017 Jan 25. PMID 27829585
  2. Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiae overexpressing alkaline phosphatase. Semkiv MV, et al. BMC Biotechnol, 2014 May 15. PMID 24884834, Free PMC Article
  3. A soluble form of phosphatase in Saccharomyces cerevisiae capable of converting farnesyl diphosphate into E,E-farnesol. Song L. Appl Biochem Biotechnol, 2006 Feb. PMID 16484724
  4. Specific dephosphorylation of phosphopeptides by the yeast alkaline phosphatase encoded by PHO8 gene. Donella-Deana A, et al. Biochim Biophys Acta, 1993 Jun 6. PMID 8499492
  5. G-protein ligands inhibit in vitro reactions of vacuole inheritance. Haas A, et al. J Cell Biol, 1994 Jul. PMID 8027189, Free PMC Article

See all (89) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
    Title: Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
  2. Results suggest that alkaline phosphatase ALP, which is encoded by PHO8, affects the polyphosphate (polyp) content, but not the chain length, and participates in polyP homeostasis in phosphate -sufficient conditions
    Title: PHO8 gene coding alkaline phosphatase of Saccharomyces cerevisiae is involved in polyphosphate metabolism.
  3. Pho8 activity and accumulation are zinc-dependent and decrease in zinc-limited cells.
    Title: Zinc status and vacuolar zinc transporters control alkaline phosphatase accumulation and activity in Saccharomyces cerevisiae.
  4. PHO8 promoter has greater nucleosome positioning power and the properly positioned nucleosomes are more stable than those at the PHO5 promoter.
    Title: Nucleosome stability at the yeast PHO5 and PHO8 promoters correlates with differential cofactor requirements for chromatin opening.
  5. Results show that the histone chaperone Asf1 increases the rate of histone eviction at the PHO5 and PHO8 promoters.
    Title: The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters.
  6. A truncated form of alkaline phosphatase Pho8 lacking 62 amino acids from the N-terminus was found to be able to convert farnesyl diphosphate (FPP) into E,E-farnesol.
    Title: A soluble form of phosphatase in Saccharomyces cerevisiae capable of converting farnesyl diphosphate into E,E-farnesol.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables alkaline phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables alkaline phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables alkaline phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables fructose-2,6-bisphosphate 6-phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in dephosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nicotinamide nucleotide metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within nucleotide catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in organophosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphate-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in fungal-type vacuole membrane HDA PubMed 
is_active_in fungal-type vacuole membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in fungal-type vacuole membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuolar membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
alkaline phosphatase PHO8
NP_010769.3
  • Repressible vacuolar alkaline phosphatase; controls polyphosphate content; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    1418550..1420250 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180789.3 → NP_010769.3  TPA: alkaline phosphatase PHO8 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010769.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VTA4, E9P949, P11491, Q03374
    UniProtKB/TrEMBL
    B3LFI5, C7GKS0, C8Z639, N1P5R2
    Conserved Domains (1) summary
    COG1785
    Location:8 → 530
    PhoA; Alkaline phosphatase [Inorganic ion transport and metabolism, General function prediction only]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P11491.2

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