SPT3 transcriptional regulator SPT3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPT3
Official Full Name: transcriptional regulator SPT3
Primary source: SGD:S000002800
Locus tag: YDR392W
See related: AllianceGenome:SGD:S000002800; FungiDB:YDR392W; VEuPathDB:YDR392W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables transcription coregulator activity. Involved in several processes, including invasive growth in response to glucose limitation; pseudohyphal growth; and transcription by RNA polymerase II. Located in cytosol and nucleus. Part of SAGA complex and SLIK (SAGA-like) complex. Orthologous to human SUPT3H (SPT3 homolog, SAGA and STAGA complex component). [provided by Alliance of Genome Resources, May 2025]
Orthologs: all
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Genomic context

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See SPT3 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1258696..1259709)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The function of Spt3, a subunit of the SAGA complex, in PGK1 transcription is restored only partially when reintroduced by plasmid into taf1 spt3 double mutant yeast strains.
Title: The function of Spt3, a subunit of the SAGA complex, in PGK1 transcription is restored only partially when reintroduced by plasmid into taf1 spt3 double mutant yeast strains.
Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
Title: Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
results represented that SPT3 was acted as global transcription regulator in the exponential phase of strain and accordingly improved heavy metal tolerance in the heterologous host S. cerevisiae
Title: Improvement of Lead Tolerance of Saccharomyces cerevisiae by Random Mutagenesis of Transcription Regulator SPT3.
Gcn5 acetyltransferase synergizes with Spt3 (TBP-binding) to promote transcription.
Title: SAGA Is a General Cofactor for RNA Polymerase II Transcription.
SAGA-TBP binding involves a network of interactions between subunits Spt3, Spt8, Spt20, and Spt7.
Title: Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex.
Gcn5p is a target regulated by the competing actions of Snf1p and Spt3p.
Title: Snf1p regulates Gcn5p transcriptional activity by antagonizing Spt3p.
SAGA and Rpd3 complexes are rapidly and synchronously recruited to heat shock factor 1-activated genes
Title: SAGA and Rpd3 chromatin modification complexes dynamically regulate heat shock gene structure and expression.
Simultaneous modulation of the expression level of SPT15 and SPT3 can increase the production of ethanol by improving osmotic tolerance and ethanol tolerance of industrial strains.
Title: Effect of overexpression of transcription factors on the fermentation properties of Saccharomyces cerevisiae industrial strains.
There is a direct interaction between TBP and the SAGA coactivator subunits Spt3 and Spt8
Title: Site-specific cross-linking of TBP in vivo and in vitro reveals a direct functional interaction with the SAGA subunit Spt3.
a direct Spt3-TATA-binding protein interaction is required for normal TATA-binding protein levels at Spt3-dependent promoters in vivo.
Title: Characterization of new Spt3 and TATA-binding protein mutants of Saccharomyces cerevisiae: Spt3 TBP allele-specific interactions and bypass of Spt8.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein heterodimerization activity	IEA Inferred from Electronic Annotation more info
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coregulator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription coregulator activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in invasive growth in response to glucose limitation	IDA Inferred from Direct Assay more info	PubMed
involved_in pseudohyphal growth	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of SAGA complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SLIK (SAGA-like) complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: transcriptional regulator SPT3

NP_010680.1

Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; role in preventing L-A mycovirus pathogenesis; relocalizes to the cytosol in response to hypoxia