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RVS167 amphiphysin [ Saccharomyces cerevisiae S288C ]

Gene ID: 851996, updated on 6-Jul-2025
Official Symbol
RVS167
Official Full Name
amphiphysin
Primary source
SGD:S000002796
Locus tag
YDR388W
See related
AllianceGenome:SGD:S000002796; FungiDB:YDR388W; VEuPathDB:YDR388W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables calmodulin binding activity and cytoskeletal protein binding activity. Contributes to lipid binding activity. Involved in several processes, including actin cortical patch localization; mitotic actomyosin contractile ring assembly; and plasma membrane tubulation. Located in actin cortical patch; cellular bud tip; and mating projection tip. Part of Rvs161p-Rvs167p complex. Orthologous to human AMPH (amphiphysin) and BIN2 (bridging integrator 2). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See RVS167 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (1250186..1251634)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Mus81p Neighboring gene Cin10p Neighboring gene GTPase-activating protein SAC7 Neighboring gene E1 ubiquitin-activating protein UBA2

Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Alteration of a yeast SH3 protein leads to conditional viability with defects in cytoskeletal and budding patterns. Bauer F, et al. Mol Cell Biol, 1993 Aug. PMID 8336735, Free PMC Article
  2. Rvs167p, the budding yeast homolog of amphiphysin, colocalizes with actin patches. Balguerie A, et al. J Cell Sci, 1999 Aug. PMID 10393809
  3. Autoimmunity in stiff-Man syndrome with breast cancer is targeted to the C-terminal region of human amphiphysin, a protein similar to the yeast proteins, Rvs167 and Rvs161. David C, et al. FEBS Lett, 1994 Aug 29. PMID 8076697
  4. A role for the dynamin-like protein Vps1 during endocytosis in yeast. Smaczynska-de Rooij II, et al. J Cell Sci, 2010 Oct 15. PMID 20841380, Free PMC Article
  5. Functional assessment of the yeast Rvs161 and Rvs167 protein domains. Sivadon P, et al. FEBS Lett, 1997 Nov 3. PMID 9395067

See all (200) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Elevated PM tension, upon TORC2 inhibition, impinges on endocytosis at two different levels by (1) severing the bonds between the PM adaptor proteins Sla2 and Ent1 and the actin cytoskeleton and (2) hindering recruitment of Rvs167, an N-BAR-containing protein important for vesicle fission to endocytosis sites
    Title: TORC2 controls endocytosis through plasma membrane tension.
  2. Genetic, bioinformatics, biochemical and biophysical data show that attraction between this alpha1-conjugated ubiquitin and the HECT ubiquitin-binding patch pulls the alpha1-helix out of the interface, thereby promoting trimerization. Strikingly, trimerization renders the ubiquitin ligase inactive.
    Title: Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.
  3. deletion of RVS167 causes downregulation of de novo sphingolipid biosynthesis via Orm2
    Title: Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels.
  4. reveal a conserved role for calmodulin in regulating the intrinsic membrane-sculpting activity of endocytic N-BAR domains, Calmodulin-binding mutants of Rvs167 exhibited defects in endocytic vesicle release
    Title: Calmodulin Promotes N-BAR Domain-Mediated Membrane Constriction and Endocytosis.
  5. 59 of 131 in: PLoS One. 2013;8(2) (dup#16) Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis
    Title: Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast.
  6. Vps1 and Rvs167 function together to mediate scission during endocytosis in Saccharomyces cerevisiae.
    Title: Yeast dynamin Vps1 and amphiphysin Rvs167 function together during endocytosis.
  7. In gyp5Deltagyl1Delta cells, Rvs167p is not correctly localized to the small-bud tip.
    Title: The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis.
  8. SUR4 does not remediate the steady-state actin cytoskeletal defects of rvs167 or rvs161 cells
    Title: Characterizing the sphingolipid signaling pathway that remediates defects associated with loss of the yeast amphiphysin-like orthologs, Rvs161p and Rvs167p.
  9. RVS161 and RVS167 have an identical set of 49 synthetic lethal interactions, revealing functions for the Rvs proteins in cell polarity, cell wall synthesis, and vesicle trafficking as well as a shared role in mating.
    Title: Characterization of the yeast amphiphysins Rvs161p and Rvs167p reveals roles for the Rvs heterodimer in vivo.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables actin binding IEA
Inferred from Electronic Annotation
more info
  
enables calmodulin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables calmodulin binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables cytoskeletal protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to lipid binding IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to lipid binding IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to lipid binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in actin cortical patch localization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin cortical patch localization IEA
Inferred from Electronic Annotation
more info
  
involved_in actin cortical patch localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endocytosis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in endocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in lipid tube assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mitotic actomyosin contractile ring assembly IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic actomyosin contractile ring assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in plasma membrane tubulation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in plasma membrane tubulation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in plasma membrane tubulation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein localization to cell division site IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in vesicle-mediated transport IPI
Inferred from Physical Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of Rvs161p-Rvs167p complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of Rvs161p-Rvs167p complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in actin cortical patch IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in actin cortical patch IDA
Inferred from Direct Assay
more info
 PubMed 
located_in actin cortical patch IEA
Inferred from Electronic Annotation
more info
  
is_active_in actin cytoskeleton IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cellular bud tip IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
located_in mating projection tip HDA PubMed 
is_active_in mating projection tip IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mating projection tip IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in medial cortex IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
amphiphysin
NP_010676.1
  • Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin; necessary for efficient trafficking of CoQ6 to mitochondria

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    1250186..1251634
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180696.1 → NP_010676.1  TPA: amphiphysin

    See identical proteins and their annotated locations for NP_010676.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VT22, P39743
    UniProtKB/TrEMBL
    A6ZYY9, B3LFR2, C7GSD4, C8Z5V2, G2WBC8
    Conserved Domains (2) summary
    cd07599
    Location:29 → 245
    BAR_Rvs167p; The Bin/Amphiphysin/Rvs (BAR) domain of Saccharomyces cerevisiae Reduced viability upon starvation protein 167 and similar proteins
    cl17036
    Location:425 → 478
    SH3; Src Homology 3 domain superfamily
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P39743.1

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