EFT2 elongation factor 2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: EFT2
Official Full Name: elongation factor 2
Primary source: SGD:S000002793
Locus tag: YDR385W
See related: AllianceGenome:SGD:S000002793; FungiDB:YDR385W; VEuPathDB:YDR385W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Predicted to enable GTPase activity; ribosome binding activity; and translation elongation factor activity. Involved in translational elongation. Is active in cytosol. Used to study spinocerebellar ataxia type 26. Human ortholog(s) of this gene implicated in glaucoma; spinocerebellar ataxia type 26; and stomach cancer. Orthologous to human EEF2 (eukaryotic translation elongation factor 2). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See EFT2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1243230..1245758)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eEF2 diphthamide modification restrains spurious frameshifting to maintain translational fidelity.
Title: eEF2 diphthamide modification restrains spurious frameshifting to maintain translational fidelity.
Chaperoning of eEF2 by Cns1 is essential for yeast viability and requires a defined subset of the Hsp90 machinery as well as the identified eEF2 recruiting factor Hgh1.
Title: The Co-chaperone Cns1 and the Recruiter Protein Hgh1 Link Hsp90 to Translation Elongation via Chaperoning Elongation Factor 2.
Hgh1 binding recruits TRiC to the C-terminal eEF2 module and prevents unproductive interactions of domain III, allowing efficient folding of the N-terminal GTPase module. eEF2 folding is completed upon dissociation of TRiC and Hgh1.
Title: Chaperone Function of Hgh1 in the Biogenesis of Eukaryotic Elongation Factor 2.
The histamine to diphthamide EFT2 substitution is important for translational accuracy and competitive cell growth in yeast.
Title: Importance of diphthamide modified EF2 for translational accuracy and competitive cell growth in yeast.
Deletion of EFM2 or EFM3 increased sensitivity to translation inhibitors, indicating changes in the ability for EF2 to interact and communicate with ribosomal components.
Title: Translational roles of elongation factor 2 protein lysine methylation.
findings show that the diphthamide-containing loop plays an important role in the ADP-ribosylation of eEF2 catalysed by toxin and also for modification of His699 by the endogenous diphthamide modification machinery
Title: The role of the diphthamide-containing loop within eukaryotic elongation factor 2 in ADP-ribosylation by Pseudomonas aeruginosa exotoxin A.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables rRNA binding	IEA Inferred from Electronic Annotation more info
enables ribosome binding	IBA Inferred from Biological aspect of Ancestor more info
enables translation elongation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation elongation factor activity	IEA Inferred from Electronic Annotation more info
enables translation elongation factor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational elongation	IEA Inferred from Electronic Annotation more info
involved_in translational elongation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: elongation factor 2

NP_010673.1

Eukaryotic translation elongation factor 2 (eEF2, EF-2); also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication