ESC2 Esc2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ESC2
Gene description: Esc2p
Primary source: SGD:S000002771
Locus tag: YDR363W
See related: AllianceGenome:SGD:S000002771
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables SUMO conjugating enzyme activity. Involved in several processes, including chromosome organization; mitotic intra-S DNA damage checkpoint signaling; and positive regulation of protein sumoylation. Located in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ESC2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1199183..1200553)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Multifaceted regulation of the sumoylation of the Sgs1 DNA helicase.
Title: Multifaceted regulation of the sumoylation of the Sgs1 DNA helicase.
Through molecular genetic analysis of this defined fork-stalling event, coupled with the use of a genome-wide genetic screen, we identified an important role for the SUMO-like domain protein, Esc2, in limiting genome rearrangements at a telomere. Moreover, we showed that these rearrangements are driven by the combined action of the Mph1 helicase and the homologous recombination machinery
Title: Esc2 promotes telomere stability in response to DNA replication stress.
The data point to the possibility that the involvement of Esc2 in the metabolism of sister chromatid junctions is, in part, via modulation of the activity of the Mus81 complex.
Title: Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains.
Reb1p indeed binds to the TFC6 promoter, and is important for proper transcription start site selection and protein expression. Interestingly, loss of Reb1p association at this site has a similar effect on the adjacent divergently transcribed ESC2 promoter, resulting in a significant increase of 5'-extended ESC2 transcripts and reduction of Esc2 protein levels.
Title: Transcription factor Reb1 is required for proper transcriptional start site usage at the divergently transcribed TFC6-ESC2 locus in Saccharomyces cerevisiae.
Tel1, Mre11 and Esc2 can silence a reporter gene dependent on the Sir, as well as on other repair proteins. Furthermore, these proteins aid in the localization of silenced domains to specific compartments in the nucleus.
Title: Heterochromatin formation via recruitment of DNA repair proteins.
Esc2 binds stalled replication forks and counteracts the anti-recombinase Srs2 helicase via a two-faceted mechanism involving chromatin recruitment and turnover of Srs2.
Title: Local regulation of the Srs2 helicase by the SUMO-like domain protein Esc2 promotes recombination at sites of stalled replication.
distinct SUMO ligases work in concert with Esc2 and Slx5-Slx8 to control substrate specificity and sumoylation homeostasis to prevent GCRs.
Title: Distinct SUMO ligases cooperate with Esc2 and Slx5 to suppress duplication-mediated genome rearrangements.
Data suggest that the Smc5/6 complex and Esc2 prevent the accumulation of toxic recombination intermediates generated in replication-associated recombinational repair.
Title: The Smc5/6 complex and Esc2 influence multiple replication-associated recombination processes in Saccharomyces cerevisiae.
Results identify a putative SUMO-binding motif in Esc2 that is necessary and sufficient for interacting with Sir2p and SUMO and is required for the function of Esc2p in transcriptional silencing.
Title: Saccharomyces cerevisiae Esc2p interacts with Sir2p through a small ubiquitin-like modifier (SUMO)-binding motif and regulates transcriptionally silent chromatin in a locus-dependent manner.
Esc2 and Smc5-6 act in concert with Sgs1 to prevent the accumulation of recombinogenic structures at damaged replication forks, likely by integrating sumoylation activities to regulate the repair pathways in response to damaged DNA
Title: The Saccharomyces cerevisiae Esc2 and Smc5-6 proteins promote sister chromatid junction-mediated intra-S repair.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables SUMO ligase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables Y-form DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables flap-structured DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of endodeoxyribonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein sumoylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Esc2p

NP_010650.1

SUMO-like domain protein; binds Holliday junctions and replication fork structures; stimulates the Mms4-Mus81 endonuclease in the resolution of sister chromatid junctions; role in recombination-mediated DNA damage tolerance by recruiting Rad51p; facilitate Elg1p binding to stalled replication forks; prevents toxic intermediate accumulation during replication-associated recombinational repair; role in silencing, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1199183..1200553

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180671.1 → NP_010650.1 TPA: Esc2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010650.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSZ1, Q06340

UniProtKB/TrEMBL

A0A8H8UNQ9, B3LFU0, C7GMH4, N1PAI6

Conserved Domains (2) summary

cd17080
Location:382 → 454

Ubl_SLD2_Esc2_like; SUMO-like domain 2 (SLD2), structurally similar to a beta-grasp ubiquitin-like fold, found in Saccharomyces cerevisiae establishes silent chromatin protein 2 (Esc2p) and similar proteins

cl21478
Location:265 → 357

ATP-synt_B; ATP synthase B/B' CF(0)