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HTB1 histone H2B [ Saccharomyces cerevisiae S288C ]

Gene ID: 851810, updated on 15-Feb-2026
Official Symbol
HTB1
Official Full Name
histone H2B
Primary source
SGD:S000002632
Locus tag
YDR224C
See related
AllianceGenome:SGD:S000002632; FungiDB:YDR224C; VEuPathDB:YDR224C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
SPT12
Summary
Enables DNA binding activity. Involved in chromatin organization; negative regulation of transcription by RNA polymerase II; and postreplication repair. Part of nucleosome. Orthologous to several human genes including H2BC1 (H2B clustered histone 1); H2BC10 (H2B clustered histone 10); and H2BC11 (H2B clustered histone 11). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See HTB1 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (914317..914712, complement)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Crf1p Neighboring gene histone H2A Neighboring gene adenylate kinase ADK1

Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription. Parra MA, et al. Mol Cell Biol, 2006 May. PMID 16648479, Free PMC Article
  2. Coding and noncoding sequences at the 3' end of yeast histone H2B mRNA confer cell cycle regulation. Xu HX, et al. Mol Cell Biol, 1990 Jun. PMID 2188095, Free PMC Article
  3. A Mutation in Histone H2B Represents a New Class of Oncogenic Driver. Bennett RL, et al. Cancer Discov, 2019 Oct. PMID 31337617, Free PMC Article
  4. A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation. Campbell SG, et al. Mol Cell Biol, 2002 Dec. PMID 12446762, Free PMC Article
  5. Chromatin assembly in a yeast whole-cell extract. Schultz MC, et al. Proc Natl Acad Sci U S A, 1997 Aug 19. PMID 9256430, Free PMC Article

See all (372) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Reversal of histone H2B mono-ubiquitination is required for replication stress recovery.
    Title: Reversal of histone H2B mono-ubiquitination is required for replication stress recovery.
  2. Influence of sequence length and charged residues on Swc5 binding with histone H2A-H2B.
    Title: Influence of sequence length and charged residues on Swc5 binding with histone H2A-H2B.
  3. RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
    Title: RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
  4. Bre1 mediates the ubiquitination of histone H2B by regulating Lge1 stability
    Title: Bre1 mediates the ubiquitination of histone H2B by regulating Lge1 stability.
  5. This study not only demonstrates the applications of the versatile histone library, but also reveals many previously unknown functions of histone H2A and H2B.
    Title: Dissecting Nucleosome Function with a Comprehensive Histone H2A and H2B Mutant Library.
  6. Data indicate that histone H2B ubiquitylation (H2Bub) and Asf1 cooperatively promote transcriptional silencing at telomeres and mating loci.
    Title: H2B ubiquitylation and the histone chaperone Asf1 cooperatively mediate the formation and maintenance of heterochromatin silencing.
  7. A Nap1 dimer provides an acidic binding surface and asymmetrically engages a single H2A-H2B heterodimer.
    Title: Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly.
  8. Chd1 takes part in chromatin reorganization surrounding transcribing RNA polymerase II (Pol II) in asymmetric nucleosomes; ubiquitin-conjugated histone H2B stimulates nucleosome sliding by Chd1.
    Title: The Chd1 chromatin remodeler shifts hexasomes unidirectionally.
  9. decreased CKII function in vivo results in decreased levels of histone H2B lysine 123 monoubiquitylation, a modification dependent on FACT and PAF-C.
    Title: Quantitative Analysis of Dynamic Protein Interactions during Transcription Reveals a Role for Casein Kinase II in Polymerase-associated Factor (PAF) Complex Phosphorylation and Regulation of Histone H2B Monoubiquitylation.
  10. Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo.
    Title: Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.
Submit: New GeneRIF Correction See all GeneRIFs (17)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein heterodimerization activity IEA
Inferred from Electronic Annotation
more info
  
enables structural constituent of chromatin IBA
Inferred from Biological aspect of Ancestor
more info
  
enables structural constituent of chromatin IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage tolerance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in chromatin organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in chromatin organization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chromatin organization NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
part_of nucleosome IDA
Inferred from Direct Assay
more info
 PubMed 
part_of nucleosome IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus NAS
Non-traceable Author Statement
more info
 PubMed 
Preferred Names
histone H2B
NP_010510.3
  • Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    914317..914712 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180532.3 → NP_010510.3  histone H2B

    See identical proteins and their annotated locations for NP_010510.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VSK6, P02293
    UniProtKB/TrEMBL
    A6ZYH8, B3LG63, C7GVC8, C8Z5D5, G2WAW9, N1P503
    Conserved Domains (1) summary
    smart00427
    Location:31 → 127
    H2B; Histone H2B
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P02293.2

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