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NUM1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851727, updated on 11-Sep-2024

Summary

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Official Symbol
NUM1
Primary source
SGD:S000002557
Locus tag
YDR150W
See related
AllianceGenome:SGD:S000002557; FungiDB:YDR150W; VEuPathDB:YDR150W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
PAC12
Summary
Enables tubulin binding activity. Involved in microtubule cytoskeleton organization; mitochondrion organization; and nuclear migration along microtubule. Located in cell cortex; cellular bud tip; and endoplasmic reticulum. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (755628..763874)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene bifunctional choline kinase/ethanolamine kinase EKI1 Neighboring gene dihydrolipoyl transsuccinylase Neighboring gene putative mRNA-binding protein CTH1 Neighboring gene Gir2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A CAAX motif can compensate for the PH domain of Num1 for cortical dynein attachment. Tang X, et al. Cell Cycle, 2009 Oct 1. PMID 19755860, Free PMC Article
  2. The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex. Heil-Chapdelaine RA, et al. J Cell Biol, 2000 Dec 11. PMID 11121446, Free PMC Article
  3. The NUM1 yeast gene: length polymorphism and physiological aspects of mutant phenotype. Revardel E, et al. Yeast, 1993 May. PMID 8322512
  4. Nuclear migration in Saccharomyces cerevisiae is controlled by the highly repetitive 313 kDa NUM1 protein. Kormanec J, et al. Mol Gen Genet, 1991 Nov. PMID 1745235
  5. Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand. Anderson HL, et al. Mol Biol Cell, 2022 Feb 1. PMID 34985939, Free PMC Article

See all (114) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mitochondria-ER-PM contacts regulate mitochondrial division and PI(4)P distribution.
    Title: Mitochondria-ER-PM contacts regulate mitochondrial division and PI(4)P distribution.
  2. Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.
    Title: Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.
  3. Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand.
    Title: Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand.
  4. Biochemical Characterization of the Num1-Mdm36 Complex at the Mitochondria-Plasma Membrane Contact Site.
    Title: Biochemical Characterization of the Num1-Mdm36 Complex at the Mitochondria-Plasma Membrane Contact Site.
  5. Cells lacking Scs2/Scs22 performed spindle positioning via MT end capture-shrinkage mechanism, requiring dynein anchorage to an ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150(Glued) subunit. Additionally, a CAAX-targeted Num1 rescued loss of lateral patches and MT sliding in the absence of Scs2/Scs22
    Title: Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1.
  6. these findings indicate that dynein activity is directly switched "on" by Num1, which induces Pac1/LIS1 removal.
    Title: The dynein cortical anchor Num1 activates dynein motility by relieving Pac1/LIS1-mediated inhibition.
  7. The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture.
    Title: The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture.
  8. Thus, our studies have revealed an additional role for Dnm1p in mitochondrial transmission through its interaction with Num1p, thereby providing a link between mitochondrial division and inheritance.
    Title: Yeast mitochondrial division and distribution require the cortical num1 protein.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables phosphatidylinositol-4,5-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phospholipid binding IEA
Inferred from Electronic Annotation
more info
  
enables tubulin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tubulin binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within intracellular distribution of mitochondria IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within intracellular distribution of mitochondria IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in maintenance of protein location in cell cortex IEA
Inferred from Electronic Annotation
more info
  
involved_in microtubule cytoskeleton organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in microtubule cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitochondrial fission IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitochondrion inheritance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitochondrion inheritance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nuclear migration along microtubule IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell cortex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell cortex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell cortex IEA
Inferred from Electronic Annotation
more info
  
is_active_in cellular bud tip IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cellular bud tip IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cellular bud tip IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in mitochondria-associated endoplasmic reticulum membrane contact site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion HDA PubMed 

General protein information

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Preferred Names
Num1p
NP_010434.1
  • Protein required for nuclear migration; component of the mitochondria-ER-cortex-anchor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    755628..763874
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180457.1 → NP_010434.1  TPA: Num1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010434.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VSD2, Q00402, Q03767
    Conserved Domains (3) summary
    pfam09528
    Location:641 → 1171
    Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)
    pfam00169
    Location:2574 → 2682
    PH; PH domain
    pfam12814
    Location:2566 → 2684
    Mcp5_PH; Meiotic cell cortex C-terminal pleckstrin homology
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q00402.2

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