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DNF2 aminophospholipid-translocating P4-type ATPase DNF2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851667, updated on 4-Feb-2026
Official Symbol
DNF2
Official Full Name
aminophospholipid-translocating P4-type ATPase DNF2
Primary source
SGD:S000002500
Locus tag
YDR093W
See related
AllianceGenome:SGD:S000002500; FungiDB:YDR093W; VEuPathDB:YDR093W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables glycosylceramide flippase activity and phosphatidylserine flippase activity. Involved in endocytosis; establishment or maintenance of cell polarity; and lipid translocation. Located in mating projection tip membrane. Is active in plasma membrane. Used to study progressive familial intrahepatic cholestasis. Orthologous to human ATP10A (ATPase phospholipid transporting 10A (putative)); ATP10B (ATPase phospholipid transporting 10B (putative)); and ATP10D (ATPase phospholipid transporting 10D (putative)). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (631282..636120)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Fe-S cluster-binding ribosome biosynthesis protein Neighboring gene E2 ubiquitin-conjugating protein UBC13 Neighboring gene histone demethylase GIS1 Neighboring gene mismatch repair ATPase MSH6

Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Local exposure of phosphatidylethanolamine on the yeast plasma membrane is implicated in cell polarity. Iwamoto K, et al. Genes Cells, 2004 Oct. PMID 15461661
  2. Uptake and utilization of lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. Riekhof WR, et al. J Biol Chem, 2006 Dec 1. PMID 17015438
  3. Lipid flippases in polarized growth. López-Marqués RL. Curr Genet, 2021 Apr. PMID 33388852
  4. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Pomorski T, et al. Mol Biol Cell, 2003 Mar. PMID 12631737, Free PMC Article
  5. Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases. Baldridge RD, et al. Proc Natl Acad Sci U S A, 2012 Feb 7. PMID 22308393, Free PMC Article

See all (66) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lipid flippases in polarized growth.
    Title: Lipid flippases in polarized growth.
  2. Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.
    Title: Exofacial membrane composition and lipid metabolism regulates plasma membrane P4-ATPase substrate specificity.
  3. Transport mechanism of P4 ATPase phosphatidylcholine flippases.
    Title: Transport mechanism of P4 ATPase phosphatidylcholine flippases.
  4. An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast.
    Title: An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast.
  5. Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
    Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.
  6. aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae
    Title: The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae.
  7. The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
    Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATPase-coupled intramembrane lipid transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATPase-coupled intramembrane lipid transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables aminophospholipid flippase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables aminophospholipid flippase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables glycosylceramide flippase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycosylceramide flippase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosylceramide flippase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylcholine floppase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylserine flippase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylserine flippase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables phosphatidylserine flippase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables phosphatidylserine floppase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in aminophospholipid translocation IEA
Inferred from Electronic Annotation
more info
  
involved_in ceramide translocation IEA
Inferred from Electronic Annotation
more info
  
involved_in ceramide translocation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in endocytosis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in endocytosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment or maintenance of cell polarity IEA
Inferred from Electronic Annotation
more info
  
involved_in establishment or maintenance of cell polarity IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in lipid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in phospholipid translocation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in phospholipid translocation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in phospholipid translocation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in phospholipid translocation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phospholipid translocation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in phospholipid transport IEA
Inferred from Electronic Annotation
more info
  
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell periphery HDA PubMed 
located_in cellular bud neck HDA PubMed 
located_in endoplasmic reticulum HDA PubMed 
located_in mating projection tip membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mating projection tip membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
part_of phospholipid-translocating ATPase complex IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
aminophospholipid-translocating P4-type ATPase DNF2
NP_010378.1
  • Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    631282..636120
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180401.1 → NP_010378.1  aminophospholipid-translocating P4-type ATPase DNF2

    See identical proteins and their annotated locations for NP_010378.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VS80, Q12675
    UniProtKB/TrEMBL
    C7GVP3, C8Z511
    Conserved Domains (1) summary
    TIGR01652
    Location:229 → 1456
    ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12675.1

Gene LinkOut

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