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MAF1 RNA polymerase III-inhibiting protein MAF1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851568, updated on 30-Jul-2025
Official Symbol
MAF1
Official Full Name
RNA polymerase III-inhibiting protein MAF1
Primary source
SGD:S000002412
Locus tag
YDR005C
See related
AllianceGenome:SGD:S000002412; FungiDB:YDR005C; VEuPathDB:YDR005C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables RNA polymerase III core binding activity. Involved in negative regulation of transcription by RNA polymerase III. Located in cytoplasm and nucleolus. Orthologous to human MAF1 (MAF1 homolog, negative regulator of RNA polymerase III). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See MAF1 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
2
Sequence:
Chromosome: IV; NC_001136.10 (456836..458103, complement)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene putative DNA-dependent ATPase RAD57 Neighboring gene Sok1p Neighboring gene phosphoribosylanthranilate isomerase TRP1

Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Lack of Maf1 enhances pyruvate kinase activity and fermentative metabolism while influencing lipid homeostasis in Saccharomyces cerevisiae. Mierzejewska J, et al. FEBS Lett, 2016 Jan. PMID 26787463
  2. Identification of proteins associated with RNA polymerase III using a modified tandem chromatin affinity purification. Nguyen NT, et al. Gene, 2015 Feb 1. PMID 25086199
  3. maf1 mutation alters the subcellular localization of the Mod5 protein in yeast. Murawski M, et al. Acta Biochim Pol, 1994. PMID 7732762
  4. Mutation in a new gene MAF1 affects tRNA suppressor efficiency in Saccharomyces cerevisiae. Boguta M, et al. Gene, 1997 Feb 7. PMID 9055829
  5. Dephosphorylation and genome-wide association of Maf1 with Pol III-transcribed genes during repression. Roberts DN, et al. Mol Cell, 2006 Jun 9. PMID 16762836, Free PMC Article

See all (94) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural basis for RNA polymerase III transcription repression by Maf1 in humans and yeast has been uncovered.
    Title: Structural basis for RNA polymerase III transcription repression by Maf1.
  2. In a mutant compromised in RNAP III activity, there is a repartitioning towards amino acids synthesis de novo at the expense of glycolytic throughput. Conversely, cells lacking Maf1 protein have greater potential for glycolytic flux.
    Title: Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1.
  3. correlation between CCA synthesis and Pol III regulation by Maf1 could be important in coordination of tRNA transcription, processing and regulation of translation
    Title: Maf1-mediated regulation of yeast RNA polymerase III is correlated with CCA addition at the 3' end of tRNA precursors.
  4. Sub1 and Maf1 also exhibit an opposite role on yeast chronological life span
    Title: Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle.
  5. PKA modulates Maf1 protein abundance for stress and lifespan but not growth
    Title: Distinct regulation of Maf1 for lifespan extension by Protein kinase A and Sch9.
  6. Hypothesized that decreased FBP1 mRNA level could be due to the effect of Maf1 on tgm silencing (tRNA gene mediated silencing).
    Title: Maf1, repressor of tRNA transcription, is involved in the control of gluconeogenetic genes in Saccharomyces cerevisiae.
  7. Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway.
    Title: Maf1-mediated repression of RNA polymerase III transcription inhibits tRNA degradation via RTD pathway.
  8. Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation.
    Title: Recovery of RNA polymerase III transcription from the glycerol-repressed state: revisiting the role of protein kinase CK2 in Maf1 phosphoregulation.
  9. Maf1-mediated transcription control and nuclear export by Los1 are two major stages of tRNA biosynthesis that are regulated by environmental conditions in a coordinated manner.
    Title: Maf1 protein, repressor of RNA polymerase III, indirectly affects tRNA processing.
  10. an important role of CK2-mediated Maf1 phosphorylation in triggering Pol III activation.
    Title: Casein kinase II-mediated phosphorylation of general repressor Maf1 triggers RNA polymerase III activation.
Submit: New GeneRIF Correction See all GeneRIFs (20)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables RNA polymerase III core binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase III core binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of transcription by RNA polymerase III IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of transcription by RNA polymerase III IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase III IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of transcription by RNA polymerase III IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase III IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase III IPI
Inferred from Physical Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
RNA polymerase III-inhibiting protein MAF1
NP_010288.1
  • Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    456836..458103 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180313.1 → NP_010288.1  TPA: RNA polymerase III-inhibiting protein MAF1

    See identical proteins and their annotated locations for NP_010288.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VRZ3, P41910
    UniProtKB/TrEMBL
    A6ZXX1, B3LGQ3, C8Z4S5, G2WCF9, N1P6A6
    Conserved Domains (1) summary
    cl02103
    Location:81 → 387
    Maf1; Maf1 regulator
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P41910.1

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