POL3 DNA-directed DNA polymerase delta POL3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: POL3
Official Full Name: DNA-directed DNA polymerase delta POL3
Primary source: SGD:S000002260
Locus tag: YDL102W
See related: AllianceGenome:SGD:S000002260; FungiDB:YDL102W; VEuPathDB:YDL102W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC2; HPR6; TEX1
Summary: Enables 3'-5'-DNA exonuclease activity and DNA-directed DNA polymerase activity. Involved in DNA metabolic process. Located in replication fork. Used to study colorectal cancer and endometrial cancer. Human ortholog(s) of this gene implicated in immunodeficiency 120. Orthologous to human POLD1 (DNA polymerase delta 1, catalytic subunit). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Genomic context

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See POL3 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (276872..280165)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mismatch repair operates at the replication fork in direct competition with mismatch extension by DNA polymerase delta.
Title: Mismatch repair operates at the replication fork in direct competition with mismatch extension by DNA polymerase δ.
Fe-S coordination defects in the replicative DNA polymerase delta cause deleterious DNA replication in vivo and subsequent DNA damage in the yeast Saccharomyces cerevisiae.
Title: Fe-S coordination defects in the replicative DNA polymerase delta cause deleterious DNA replication in vivo and subsequent DNA damage in the yeast Saccharomyces cerevisiae.
Mitochondrial fusion and fission are required for proper mitochondrial function and cell proliferation in fission yeast.
Title: Mitochondrial fusion and fission are required for proper mitochondrial function and cell proliferation in fission yeast.
Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.
Title: Limiting DNA polymerase delta alters replication dynamics and leads to a dependence on checkpoint activation and recombination-mediated DNA repair.
Study show that, in Saccharomyces cerevisiae, the mutation rate increases synergistically when a Polepsilon nucleotide selectivity defect is combined with a Poldelta proofreading defect, demonstrating extrinsic proofreading of Polepsilon errors by Poldelta. Combining Poldelta nucleotide selectivity and Polepsilon proofreading defects produces no synergy, indicating that Polepsilon cannot correct errors made by Poldelta.
Title: DNA polymerase δ proofreads errors made by DNA polymerase ε.
Pol delta replicates both the invading and the complementary strand during break-induced replication.
Title: DNA Polymerase Delta Synthesizes Both Strands during Break-Induced Replication.
The structure reveals an unexpected arrangement in which the regulatory subunits (Pol31 and Pol32) lie next to the exonuclease domain of Pol3 but do not engage the DNA.
Title: Cryo-EM structure and dynamics of eukaryotic DNA polymerase δ holoenzyme.
Complementary roles of Pif1 helicase and single stranded DNA binding proteins RPA and DNA polymerase subunits in stimulating DNA replication through G-quadruplexes have been demonstrated.
Title: Complementary roles of Pif1 helicase and single stranded DNA binding proteins in stimulating DNA replication through G-quadruplexes.
We found that C37/53 sensitizes RNAP III termination to RNA:DNA hybrid strength and promotes RNA 3' end pairing/annealing with the template. The latter counteracts C11-insensitive arrest in the proximal part of the oligo(T)-tract, promoting oligo(rU:dA) extension toward greater hybrid instability and RNA release
Title: RNA polymerase III subunits C37/53 modulate rU:dA hybrid 3' end dynamics during transcription termination.
Normally lethal amino acid substitutions suppress an ultramutator DNA Polymerase delta variant.
Title: Normally lethal amino acid substitutions suppress an ultramutator DNA Polymerase δ variant.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5'-DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5'-DNA exonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication proofreading	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication proofreading	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication, removal of RNA primer	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA-templated DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated DNA replication maintenance of fidelity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in RNA-templated DNA biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair, gap-filling	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair, DNA gap filling	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of delta DNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of delta DNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of delta DNA polymerase complex	TAS Traceable Author Statement more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in replication fork	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA-directed DNA polymerase delta POL3

NP_010181.2

EC 2.7.7.7

Catalytic subunit of DNA polymerase delta; responsible for most of lagging strand DNA synthesis; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

276872..280165

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180161.2 → NP_010181.2 TPA: DNA-directed DNA polymerase delta POL3

See identical proteins and their annotated locations for NP_010181.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRP8, P15436

UniProtKB/TrEMBL

A6ZXM7, C8Z6K2

Conserved Domains (1) summary

PTZ00166
Location:90 → 1095

PTZ00166; DNA polymerase delta catalytic subunit; Provisional