QRI7 putative N(6)-L-threonylcarbamoyladenine synthase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: QRI7
Official Full Name: putative N(6)-L-threonylcarbamoyladenine synthase
Primary source: SGD:S000002262
Locus tag: YDL104C
See related: AllianceGenome:SGD:S000002262; FungiDB:YDL104C; VEuPathDB:YDL104C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables N(6)-L-threonylcarbamoyladenine synthase activity; nucleotidase activity; and tRNA binding activity. Involved in mitochondrial tRNA threonylcarbamoyladenosine modification. Located in mitochondrion. Orthologous to human OSGEPL1 (O-sialoglycoprotein endopeptidase like 1). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Genomic context

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See QRI7 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (273653..274876, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our results firmly established that Qri7p and Sua5p constitute the mitochondrial pathway for the biosynthesis of t(6)A and bring additional advancement in our understanding of the reaction mechanism
Title: Cross kingdom functional conservation of the core universally conserved threonylcarbamoyladenosine tRNA synthesis enzymes.
The manner of AMP recognition is similar, with some minor variations, among the Qri7/Kae1/YgjD family proteins
Title: Structure of Saccharomyces cerevisiae mitochondrial Qri7 in complex with AMP.
Qri7/OSGEPL protein localizes to the mitochondria and is involved in mitochondrial genome maintenance in the model eukaryotic organism, Saccharomyces cerevisiae.
Title: Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables acyltransferase activity, transferring groups other than amino-acyl groups	IEA Inferred from Electronic Annotation more info
enables nucleotidase activity	IDA Inferred from Direct Assay more info	PubMed
enables tRNA N(6)-L-threonylcarbamoyladenine synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables tRNA N(6)-L-threonylcarbamoyladenine synthase activity	IEA Inferred from Electronic Annotation more info
enables tRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in mitochondrial tRNA threonylcarbamoyladenosine modification	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial tRNA threonylcarbamoyladenosine modification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA threonylcarbamoyladenosine modification	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA threonylcarbamoyladenosine modification	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: putative N(6)-L-threonylcarbamoyladenine synthase

NP_010179.1

EC 2.3.1.234

Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

273653..274876 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180163.1 → NP_010179.1 putative N(6)-L-threonylcarbamoyladenine synthase

See identical proteins and their annotated locations for NP_010179.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRP6, P43122

UniProtKB/TrEMBL

A6ZXM5, C8Z6K0

Conserved Domains (1) summary

TIGR00329
Location:36 → 367

gcp_kae1; metallohydrolase, glycoprotease/Kae1 family