KIN28 TFIIH complex serine/threonine-protein kinase subunit KIN28 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Gene symbol: KIN28
Gene description: TFIIH complex serine/threonine-protein kinase subunit KIN28
Primary source: SGD:S000002266
Locus tag: YDL108W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See KIN28 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 2

Sequence:: Chromosome: IV; NC_001136.10 (267698..268699)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inactivating Kin28 impacts promoter release to differing degrees and reveals a "checkpoint" during the transition to productive elongation, resulting in widespread mRNA stabilization.
Title: Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization.
Kin28 has a role in the association of Mediator with promoters.
Title: Kin28 regulates the transient association of Mediator with core promoters.
These results identify an Spt5 phosphorylated C-terminal repeats-independent pathway of Paf1C recruitment requiring Kin28, Bur1, and the Spt5 phosphorylated C-terminal repeats-interaction domain of Cdc73.
Title: Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex.
Study points strongly toward a minor role of TFIIH kinase in Pol II transcription, and a more significant role in mRNA capping in budding yeast.
Title: Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.
Results implicate the TFIIH kinase in placing bivalent Ser5 and Ser7 marks early in gene transcription.
Title: TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II.
These results suggest that the overexpression of Kin28 and Pog1 enhances the protein refolding and degradation pathways in rsp5(A401E) cells.
Title: Overexpression of two transcriptional factors, Kin28 and Pog1, suppresses the stress sensitivity caused by the rsp5 mutation in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Homologs of the KIN28 gene: The KIN28 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and frog.

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cyclin-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 7-methylguanosine mRNA capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in cellular response to nitrogen starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in phosphorylation of RNA polymerase II C-terminal domain	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in phosphorylation of RNA polymerase II C-terminal domain	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphorylation of RNA polymerase II C-terminal domain	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex	IPI Inferred from Physical Interaction more info	PubMed
involved_in phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in positive regulation of transcription elongation from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Atg1/ULK1 kinase complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	HDA more info	PubMed
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell cycle	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIH holo complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIH holo complex	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIK complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
part_of transcription factor TFIIK complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIK complex	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: TFIIH complex serine/threonine-protein kinase subunit KIN28

NP_010175.1

EC 2.7.11.23

Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly; phosphorylates the C-terminal domain (CTD) of RNAPII (Ser5 and Ser7); facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex; minor role in RNAPII transcription; role in RNAPI transcription; regulates Atg1p kinase complex assembly during nitrogen starvation induced autophagy; associates with cyclin Ccl1p; relocalizes to the cytosol in response to hypoxia; ortholog of human CDK7

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

267698..268699

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180167.1 → NP_010175.1 TPA: TFIIH complex serine/threonine-protein kinase subunit KIN28 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010175.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P06242

Conserved Domains (1) summary

cd07841
Location:7 → 302

STKc_CDK7; Catalytic domain of the Serine/Threonine Kinase, Cyclin-Dependent protein Kinase 7