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LYS20 homocitrate synthase LYS20 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851346, updated on 7-Jun-2026
Official Symbol
LYS20
Official Full Name
homocitrate synthase LYS20
Primary source
SGD:S000002341
Locus tag
YDL182W
See related
AllianceGenome:SGD:S000002341; FungiDB:YDL182W; VEuPathDB:YDL182W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables homocitrate synthase activity. Involved in DNA repair and lysine biosynthetic process via aminoadipic acid. Located in nucleus. Human ortholog(s) of this gene implicated in 3-hydroxy-3-methylglutaryl-CoA lyase deficiency and amino acid metabolic disorder. Orthologous to human HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) and HMGCLL1 (3-hydroxy-3-methylglutaryl-CoA lyase like 1). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See LYS20 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (133437..134723)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene 60S ribosomal protein eL41 RPL41A Neighboring gene Mrx19p Neighboring gene ATPase inhibitor Neighboring gene Ait1p

Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Homocitrate synthase from yeast. Tucci AF, et al. Arch Biochem Biophys, 1972 Dec. PMID 4575437
  2. The Lys20 homocitrate synthase isoform exerts most of the flux control over the lysine synthesis pathway in Saccharomyces cerevisiae. Quezada H, et al. Mol Microbiol, 2011 Nov. PMID 21895798
  3. Acid-base chemical mechanism of homocitrate synthase from Saccharomyces cerevisiae. Qian J, et al. Biochemistry, 2006 Oct 3. PMID 17002313
  4. Lysine-overproducing mutants of Saccharomyces cerevisiae baker's yeast isolated in continuous culture. Gasent-Ramírez JM, et al. Appl Environ Microbiol, 1997 Dec. PMID 9406398, Free PMC Article
  5. High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase. Isogai S, et al. Appl Environ Microbiol, 2021 Jul 13. PMID 33990312, Free PMC Article

See all (87) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Lys20 appears to have evolved as a bifunctional protein that connects cellular metabolism with chromatin functions
    Title: Homocitrate synthase connects amino acid metabolism to chromatin functions through Esa1 and DNA damage.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables acyltransferase activity, acyl groups converted into alkyl on transfer IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables homocitrate synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables homocitrate synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables homocitrate synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables homocitrate synthase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables homocitrate synthase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables homocitrate synthase activity RCA
inferred from Reviewed Computational Analysis
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA repair IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in L-lysine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in L-lysine biosynthetic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in L-lysine biosynthetic process RCA
inferred from Reviewed Computational Analysis
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol RCA
inferred from Reviewed Computational Analysis
more info
  
located_in mitochondrion HDA PubMed 
located_in nucleus HDA PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
homocitrate synthase LYS20
NP_010099.1
  • Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    133437..134723
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180242.1 → NP_010099.1  homocitrate synthase LYS20

    See identical proteins and their annotated locations for NP_010099.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VRH0, P48570
    UniProtKB/TrEMBL
    A6ZXE9, B3LH66, B5VF91, C8Z6C2, G2WBY3, N1PAJ0
    Conserved Domains (1) summary
    cd07948
    Location:23 → 284
    DRE_TIM_HCS; Saccharomyces cerevisiae homocitrate synthase and related proteins, catalytic TIM barrel domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P48570.1

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