CWC2 active spliceosome conformation promoter CWC2 [Saccharomyces cerevisiae S288C] - Gene

Summary

Official Symbol: CWC2
Official Full Name: active spliceosome conformation promoter CWC2
Primary source: SGD:S000002368
Locus tag: YDL209C
See related: AllianceGenome:SGD:S000002368; FungiDB:YDL209C; VEuPathDB:YDL209C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: NTC40
Summary: Enables U6 snRNA binding activity. Involved in mRNA splicing, via spliceosome. Part of Prp19 complex; U2-type catalytic step 1 spliceosome; and U2-type catalytic step 2 spliceosome. [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

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The study found that one function of Cwc2 is to stabilize U2-U6 snRNA helix I during splicing.
Title: Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2.
The S. cerevisiae Cwc2 protein functions prior to step 1 of splicing.
Title: Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre.
we report the 1.9 A (1 A=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding.
Title: Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition.
Yeast cells depleted of Cwc2 accumulate pre-mRNA and display reduced levels of U1, U4, U5 and U6 snRNAs.
Title: The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing.

Submit: New GeneRIF Correction

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables U6 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U6 snRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables pre-mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables pre-mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in mRNA cis splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA cis splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA splicing, via spliceosome	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of RNA splicing	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spliceosomal snRNP assembly	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of Prp19 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Prp19 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of U2-type catalytic step 1 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
part_of U2-type catalytic step 1 spliceosome	IDA Inferred from Direct Assay more info	PubMed
part_of U2-type catalytic step 2 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
part_of U2-type catalytic step 2 spliceosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

NP_010072.1

Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2

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