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NAM2 leucine--tRNA ligase NAM2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851098, updated on 3-Jun-2026
Official Symbol
NAM2
Official Full Name
leucine--tRNA ligase NAM2
Primary source
SGD:S000004374
Locus tag
YLR382C
See related
AllianceGenome:SGD:S000004374; FungiDB:YLR382C; VEuPathDB:YLR382C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
MSL1
Summary
Enables leucine-tRNA ligase activity and pre-mRNA intronic binding activity. Involved in Group I intron splicing; leucyl-tRNA aminoacylation; and mitochondrial translation. Located in mitochondrion. Used to study Perrault syndrome and mitochondrial metabolism disease. Human ortholog(s) of this gene implicated in Perrault syndrome. Orthologous to human LARS2 (leucyl-tRNA synthetase 2, mitochondrial). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See NAM2 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (882067..884751, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Csr1p Neighboring gene Ctf3p Neighboring gene DNA repair protein SMC6 Neighboring gene Elongator subunit IKI3

Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. In vitro mutagenesis of the mitochondrial leucyl-tRNA synthetase of S. cerevisiae reveals residues critical for its in vivo activities. Li GY, et al. Curr Genet, 1992 Jul. PMID 1611670
  2. Purification and characterization of the Saccharomyces cerevisiae mitochondrial leucyl-tRNA synthetase. Zagorski W, et al. J Biol Chem, 1991 Feb 5. PMID 1990003
  3. The yeast mitochondrial leucyl-tRNA synthetase is a splicing factor for the excision of several group I introns. Labouesse M. Mol Gen Genet, 1990 Nov. PMID 2277640
  4. Homology of yeast mitochondrial leucyl-tRNA synthetase and isoleucyl- and methionyl-tRNA synthetases of Escherichia coli. Tzagoloff A, et al. J Biol Chem, 1988 Jan 15. PMID 2826465
  5. The yeast nuclear gene NAM2 is essential for mitochondrial DNA integrity and can cure a mitochondrial RNA-maturase deficiency. Labouesse M, et al. Cell, 1985 May. PMID 2986842

See all (61) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain has been reported.
    Title: Self-protective responses to norvaline-induced stress in a leucyl-tRNA synthetase editing-deficient yeast strain.
  2. Data show that the activity of the minimized bI4 intron was enhanced in vitro by the presence of the bI4 maturase or LeuRS.
    Title: Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.
  3. yeast mitochondria have evolved to tolerate lower levels of fidelity in protein synthesis or have developed alternate mechanisms to enhance discrimination of leucine from non-cognate amino acids that can be misactivated by leucyl-tRNA synthetase
    Title: A viable amino acid editing activity in the leucyl-tRNA synthetase CP1-splicing domain is not required in the yeast mitochondria.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA deacylase activity IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables leucine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables leucine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables leucine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables pre-mRNA intronic binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in Group I intron splicing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in Group I intron splicing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in aminoacyl-tRNA metabolism involved in translational fidelity IEA
Inferred from Electronic Annotation
more info
  
involved_in leucyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in leucyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in leucyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mitochondrial translation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitochondrial translation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in mitochondrial matrix EXP
Inferred from Experiment
more info
 PubMed 
located_in mitochondrial matrix IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion NAS
Non-traceable Author Statement
more info
 PubMed 
Preferred Names
leucine--tRNA ligase NAM2
NP_013486.3
  • Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    882067..884751 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182271.3 → NP_013486.3  leucine--tRNA ligase NAM2

    See identical proteins and their annotated locations for NP_013486.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VZ17, P11325
    UniProtKB/TrEMBL
    B3RHP7, C7GXI6, C8ZDZ2, N1P047
    Conserved Domains (1) summary
    TIGR00396
    Location:21 → 893
    leuS_bact; leucyl-tRNA synthetase, eubacterial and mitochondrial family
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P11325.1

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