MCM5 MCM DNA helicase complex subunit MCM5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MCM5
Gene description: MCM DNA helicase complex subunit MCM5
Primary source: SGD:S000004264
Locus tag: YLR274W
Gene type: protein coding
RNA name: MCM DNA helicase complex subunit MCM5
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BOB1; CDC46

Genomic context

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See MCM5 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (691555..693882)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

The Mcm ATPase active sites that flank Mcm2/5 (i.e. Mcm5/3 and 6/2) modulate the biochemical activities of the putative Mcm2/5 gate.
Title: The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.
DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
Conclusions predict a structural discontinuity between Mcm2 and Mcm5 and demonstrate that in contrast to other hexameric helicases, the six Mcm2-7 active sites are functionally distinct.
Title: Subunit organization of Mcm2-7 and the unequal role of active sites in ATP hydrolysis and viability.
Mcm5 protein has a positive role in origin binding, which requires coordination by all six Mcm2-7 subunits in the hexamer.
Title: Functional conservation of beta-hairpin DNA binding domains in the Mcm protein of Methanobacterium thermoautotrophicum and the Mcm5 protein of Saccharomyces cerevisiae.
A domain-push model is proposed to explain the bypass activity of mutant proline62leucine BOB1, which has a major effect on helicase activity, but no significant impact on DNA binding or ATPase of Methanobacterium thermoautotrophicum MCM gene.
Title: Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM.

Submit: New GeneRIF Correction

Pathways from BioSystems

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Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
Activation of the pre-replicative complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle and Cell Division, organism-specific biosystem (from WikiPathways)
Cell Cycle and Cell Division, organism-specific biosystem(From http://en.wikipedia.org/wiki/Cell_cycle) The cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle in...
Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
Cell Cycle, Mitotic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell cycle - yeast, organism-specific biosystem (from KEGG)
Cell cycle - yeast, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
Cell cycle - yeast, conserved biosystem (from KEGG)
Cell cycle - yeast, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
DNA Replication, organism-specific biosystem (from WikiPathways)
DNA Replication, organism-specific biosystemDNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. This process is "replication" in that each strand of the original ...
DNA Replication, organism-specific biosystem (from REACTOME)
DNA Replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
DNA Replication Pre-Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA replication, organism-specific biosystem (from KEGG)
DNA replication, organism-specific biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA replication, conserved biosystem (from KEGG)
DNA replication, conserved biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA strand elongation, organism-specific biosystem (from REACTOME)
DNA strand elongation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G1/S Transition, organism-specific biosystem (from REACTOME)
G1/S Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
M/G1 Transition, organism-specific biosystem (from REACTOME)
M/G1 Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
MCM complex, organism-specific biosystem (from KEGG)
MCM complex, organism-specific biosystemStructural complex; Genetic information processing; Replication system
MCM complex, conserved biosystem (from KEGG)
MCM complex, conserved biosystemStructural complex; Genetic information processing; Replication system
Meiosis - yeast, organism-specific biosystem (from KEGG)
Meiosis - yeast, organism-specific biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Meiosis - yeast, conserved biosystem (from KEGG)
Meiosis - yeast, conserved biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
Mitotic G1-G1/S phases, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Orc1 removal from chromatin, organism-specific biosystem (from REACTOME)
Orc1 removal from chromatin, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Regulation of DNA replication, organism-specific biosystem (from REACTOME)
Regulation of DNA replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Removal of licensing factors from origins, organism-specific biosystem (from REACTOME)
Removal of licensing factors from origins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
S Phase, organism-specific biosystem (from REACTOME)
S Phase, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Switching of origins to a post-replicative state, organism-specific biosystem (from REACTOME)
Switching of origins to a post-replicative state, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Synthesis of DNA, organism-specific biosystem (from REACTOME)
Synthesis of DNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Unwinding of DNA, organism-specific biosystem (from REACTOME)
Unwinding of DNA, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the MCM5 gene: The MCM5 gene is conserved in human, chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
DNA replication origin binding	IEA Inferred from Electronic Annotation more info
chromatin binding	IDA Inferred from Direct Assay more info	PubMed
helicase activity	IEA Inferred from Electronic Annotation more info
hydrolase activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to single-stranded DNA-dependent ATP-dependent DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
DNA replication	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
cell cycle	IEA Inferred from Electronic Annotation more info
chromatin silencing at telomere	IMP Inferred from Mutant Phenotype more info	PubMed
double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
establishment of chromatin silencing	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of chromatin silencing at telomere	IMP Inferred from Mutant Phenotype more info	PubMed
nuclear DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
regulation of DNA-dependent DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
CMG complex	IDA Inferred from Direct Assay more info	PubMed
DNA replication preinitiation complex	IPI Inferred from Physical Interaction more info	PubMed
MCM complex	IDA Inferred from Direct Assay more info	PubMed
MCM complex	IEA Inferred from Electronic Annotation more info
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
nuclear chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	IEA Inferred from Electronic Annotation more info
replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: MCM DNA helicase complex subunit MCM5

NP_013376.1

EC 3.6.4.12

Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

691555..693882

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182161.1 → NP_013376.1 TPA: MCM DNA helicase complex subunit MCM5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013376.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P29496

Conserved Domains (2) summary

smart00350
Location:144 → 689

MCM; minichromosome maintenance proteins

pfam14551
Location:31 → 101

MCM_N; MCM N-terminal domain