HRD3 ubiquitin ligase complex subunit HRD3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: HRD3
Official Full Name: ubiquitin ligase complex subunit HRD3
Primary source: SGD:S000004197
Locus tag: YLR207W
See related: AllianceGenome:SGD:S000004197; FungiDB:YLR207W; VEuPathDB:YLR207W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to ubiquitin-protein transferase activity. Involved in negative regulation of protein autoubiquitination and retrograde protein transport, ER to cytosol. Located in endoplasmic reticulum membrane. Part of Hrd1p ubiquitin ligase ERAD-L complex; Hrd1p ubiquitin ligase ERAD-M complex; and luminal surveillance complex. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to several human genes including SEL1L (SEL1L adaptor subunit of SYVN1 ubiquitin ligase). [provided by Alliance of Genome Resources, May 2025]
Orthologs: all
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Genomic context

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See HRD3 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (556788..559289)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3: results suggest that Hrd1 forms a retro-translocation channel for the movement of misfolded polypeptides through the endoplasmic reticulum membrane
Title: Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3.
direct component of Hrd3 is phenotypically as important as Hrd1 in the native HRD complex. Hrd3 was required the E3 activity of Hrd1, rather than substrate or E2 recruitment to Hrd1. Although Hrd1 can function in some circumstances independent of Hrd3, these studies show an indispensable role for Hrd3 in living cells.
Title: Direct and essential function for Hrd3 in ER-associated degradation.
Data indicate that lysine arginine (KR) mutation causes moderate structural changes in Hrd3 protein.
Title: The interplay of Hrd3 and the molecular chaperone system ensures efficient degradation of malfolded secretory proteins.
Data show that Der1p appears to have a role in a pathway that is parallel to that involving Hrd3p.
Title: Recognition of an ERAD-L substrate analyzed by site-specific in vivo photocrosslinking.
Results suggest that Hrd3p recognises proteins that deviate from their native conformation.
Title: A complex of Yos9p and the HRD ligase integrates endoplasmic reticulum quality control into the degradation machinery.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ERAD pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein autoubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in retrograde protein transport, ER to cytosol	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Hrd1p ubiquitin ligase ERAD-L complex	IDA Inferred from Direct Assay more info	PubMed
part_of Hrd1p ubiquitin ligase ERAD-M complex	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IPI Inferred from Physical Interaction more info	PubMed
part_of luminal surveillance complex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin ligase complex subunit HRD3

NP_013308.1

ER membrane protein that plays a central role in ERAD; forms HRD complex with Hrd1p and ER-associated protein degradation (ERAD) determinants that engages in lumen to cytosol communication and coordination of ERAD events

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

556788..559289

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182094.1 → NP_013308.1 TPA: ubiquitin ligase complex subunit HRD3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013308.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYK8, Q05787

UniProtKB/TrEMBL

G2WJ37, N1P6T7

Conserved Domains (2) summary

COG0790
Location:532 → 663

TPR; TPR repeat [Signal transduction mechanisms]

sd00010
Location:537 → 564

SLR; SLR repeat [structural motif]