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RFX1 Rfx1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850873, updated on 23-Apr-2026
Official Symbol
RFX1
Official Full Name
Rfx1p
Primary source
SGD:S000004166
Locus tag
YLR176C
See related
AllianceGenome:SGD:S000004166; FungiDB:YLR176C; VEuPathDB:YLR176C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
CRT1
Summary
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Orthologous to several human genes including RFX2 (regulatory factor X2) and RFX4 (regulatory factor X4). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See RFX1 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (507797..510232, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene isocitrate dehydrogenase (NADP(+)) IDP2 Neighboring gene pseudouridine synthase CBF5 Neighboring gene uncharacterized protein Neighboring gene Tfs1p

Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The dimerization/repression domain of RFX1 is related to a conserved region of its yeast homologues Crt1 and Sak1: a new function for an ancient motif. Katan-Khaykovich Y, et al. J Mol Biol, 1999 Nov 19. PMID 10556033
  2. Candida albicans RFX2 encodes a DNA binding protein involved in DNA damage responses, morphogenesis, and virulence. Hao B, et al. Eukaryot Cell, 2009 Apr. PMID 19252121, Free PMC Article
  3. Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae. Golla U, et al. PLoS One, 2013. PMID 23741394, Free PMC Article
  4. Autorepression of rfx1 gene expression: functional conservation from yeast to humans in response to DNA replication arrest. Lubelsky Y, et al. Mol Cell Biol, 2005 Dec. PMID 16287876, Free PMC Article
  5. Excess histone levels mediate cytotoxicity via multiple mechanisms. Singh RK, et al. Cell Cycle, 2010 Oct 15. PMID 20948314, Free PMC Article

See all (77) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. two-step activation model of the DNA damage-inducible genes and that Crt1 functions as a signal-dependent dual-transcription activator and repressor that acts in a transient manner
    Title: Molecular genetic analysis of the yeast repressor Rfx1/Crt1 reveals a novel two-step regulatory mechanism.
  2. evidence for a common mechanism for Crt1 and Rfx1 expression and for the conservation of their mode of action in response to a DNA replication block in yeasts and humans
    Title: Autorepression of rfx1 gene expression: functional conservation from yeast to humans in response to DNA replication arrest.
  3. Crt1p may influence deoxyribonucleotide synthesis not only by regulating expression of the ribonucleotide reductase genes but also by modulating dihydrofolate reductase activity
    Title: Identification of new genes regulated by the Crt1 transcription factor, an effector of the DNA damage checkpoint pathway in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding HDA PubMed 
Process Evidence Code Pubs
involved_in negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in nucleus HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
Rfx1p
NP_013277.1
  • Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    507797..510232 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182063.1 → NP_013277.1  Rfx1p

    See identical proteins and their annotated locations for NP_013277.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYI0, P48743
    UniProtKB/TrEMBL
    G2WJ09
    Conserved Domains (1) summary
    pfam02257
    Location:282 → 358
    RFX_DNA_binding; RFX DNA-binding domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P48743.2

Gene LinkOut

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