CDC45 DNA replication initiation factor CDC45 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CDC45
Gene description: DNA replication initiation factor CDC45
Primary source: SGD:S000004093
Locus tag: YLR103C
Gene type: protein coding
RNA name: DNA replication initiation factor CDC45
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SLD4

Genomic context

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See CDC45 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (343989..345941, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Data show that Dbf4-dependent kinase (DDK)-phosphoryled MCM DNA helicase complex subunit MCM2 preferentially interacts with DNA replication initiation factor CDC45.
Title: Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase.
Upon extrusion of ssDNA from the central channel of Mcm2-7, Dpb11 dissociates from Mcm2-7, and Dpb11 binds to ssDNA, thereby allowing GINS to bind to Cdc45.Mcm2-7
Title: Dpb11 protein helps control assembly of the Cdc45·Mcm2-7·GINS replication fork helicase.
cdc45-ssDNA interaction is important for stalling the helicase during replication stress
Title: Cdc45 protein-single-stranded DNA interaction is important for stalling the helicase during replication stress.
The low-abundance replication proteins Sld3, Sld7, and Cdc45 form a complex and associate with the early-firing origins in G1 phase in a manner that depends on Dbf4-dependent kinase (DDK), which is essential for the initiation of DNA replication.
Title: Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing.
GINS (Psf1, Psf2, Psf3, and Sld5) and Sld3 compete with one another for Mcm2-7 and Cdc45 binding
Title: GINS and Sld3 compete with one another for Mcm2-7 and Cdc45 binding.

Submit: New GeneRIF Correction

Pathways from BioSystems

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Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
Activation of the pre-replicative complex, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle and Cell Division, organism-specific biosystem (from WikiPathways)
Cell Cycle and Cell Division, organism-specific biosystem(From http://en.wikipedia.org/wiki/Cell_cycle) The cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle in...
Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
Cell Cycle, Mitotic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell cycle - yeast, organism-specific biosystem (from KEGG)
Cell cycle - yeast, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
Cell cycle - yeast, conserved biosystem (from KEGG)
Cell cycle - yeast, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps (G1 and G2 phases). G1, S, and G...
DNA Replication, organism-specific biosystem (from WikiPathways)
DNA Replication, organism-specific biosystemDNA replication, the basis for biological inheritance, is a fundamental process occurring in all living organisms to copy their DNA. This process is "replication" in that each strand of the original ...
DNA Replication, organism-specific biosystem (from REACTOME)
DNA Replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
DNA Replication Pre-Initiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
E2F mediated regulation of DNA replication, organism-specific biosystem (from REACTOME)
E2F mediated regulation of DNA replication, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G1/S Transition, organism-specific biosystem (from REACTOME)
G1/S Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G1/S-Specific Transcription, organism-specific biosystem (from REACTOME)
G1/S-Specific Transcription, organism-specific biosystemcomputationally inferred pathway (not manually curated)
M/G1 Transition, organism-specific biosystem (from REACTOME)
M/G1 Transition, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Meiosis - yeast, organism-specific biosystem (from KEGG)
Meiosis - yeast, organism-specific biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Meiosis - yeast, conserved biosystem (from KEGG)
Meiosis - yeast, conserved biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, one pair of sister chromatids recombines with, th...
Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
Mitotic G1-G1/S phases, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Homologs of the CDC45 gene: The CDC45 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by GO

Function	Evidence Code	Pubs
3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
chromatin binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
chromatin binding	IDA Inferred from Direct Assay more info	PubMed
single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
DNA replication	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA replication initiation	IEA Inferred from Electronic Annotation more info
DNA replication initiation	IGI Inferred from Genetic Interaction more info	PubMed
DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
cell cycle	IEA Inferred from Electronic Annotation more info
double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info	PubMed
double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
mitotic DNA replication preinitiation complex assembly	IBA Inferred from Biological aspect of Ancestor more info	PubMed
positive regulation of G1/S transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IBA Inferred from Biological aspect of Ancestor more info	PubMed
pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IPI Inferred from Physical Interaction more info	PubMed
regulation of chromatin silencing at telomere	IBA Inferred from Biological aspect of Ancestor more info	PubMed
regulation of chromatin silencing at telomere	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
CMG complex	IDA Inferred from Direct Assay more info	PubMed
DNA replication preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
nuclear pre-replicative complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	IEA Inferred from Electronic Annotation more info
replication fork protection complex	IBA Inferred from Biological aspect of Ancestor more info	PubMed
replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA replication initiation factor CDC45

NP_013204.1

DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

343989..345941 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181990.1 → NP_013204.1 TPA: DNA replication initiation factor CDC45 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013204.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q08032

Conserved Domains (1) summary

pfam02724
Location:28 → 646

CDC45; CDC45-like protein