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UBI4 ubiquitin [ Saccharomyces cerevisiae S288C ]

Gene ID: 850620, updated on 5-Jul-2025
Official Symbol
UBI4
Official Full Name
ubiquitin
Primary source
SGD:S000003962
Locus tag
YLL039C
See related
AllianceGenome:SGD:S000003962; FungiDB:YLL039C; VEuPathDB:YLL039C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
SCD2; UB14
Summary
Enables protein tag activity. Involved in protein ubiquitination. Located in peroxisome. Orthologous to human UBC (ubiquitin C). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See UBI4 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (64062..65207, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene succinate dehydrogenase iron-sulfur protein subunit SDH2 Neighboring gene membrane morphogenesis protein VPS13 Neighboring gene Ent4p Neighboring gene E3 ubiquitin-protein ligase PRP19

Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Multiple independent regulatory pathways control UBI4 expression after heat shock in Saccharomyces cerevisiae. Simon JR, et al. Mol Microbiol, 1999 Feb. PMID 10048026
  2. Aromatic residues engineered into the beta-turn nucleation site of ubiquitin lead to a complex folding landscape, non-native side-chain interactions, and kinetic traps. Rea AM, et al. Biochemistry, 2008 Dec 2. PMID 18991391
  3. Functional characterization of dosage-dependent lethal mutation of ubiquitin in Saccharomyces cerevisiae. Doshi A, et al. FEMS Yeast Res, 2014 Nov. PMID 25195938
  4. Polyubiquitin gene expression contributes to oxidative stress resistance in respiratory yeast (Saccharomyces cerevisiae). Cheng L, et al. Mol Gen Genet, 1994 May 10. PMID 8190089
  5. Q2N and E64G double mutation of ubiquitin confers a stress sensitive phenotype on Saccharomyces cerevisiae. Sharma M, et al. Indian J Exp Biol, 2015 Sep. PMID 26548082

See all (408) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Systematic profiling of dominant ubiquitin variants reveals key functional nodes contributing to evolutionary selection.
    Title: Systematic profiling of dominant ubiquitin variants reveals key functional nodes contributing to evolutionary selection.
  2. A novel recognition site for polyubiquitin and ubiquitin-like signals in an unexpected region of proteasomal subunit Rpn1.
    Title: A novel recognition site for polyubiquitin and ubiquitin-like signals in an unexpected region of proteasomal subunit Rpn1.
  3. Programmed ubiquitin acetylation using genetic code expansion reveals altered ubiquitination patterns.
    Title: Programmed ubiquitin acetylation using genetic code expansion reveals altered ubiquitination patterns.
  4. These results indicate that ubiquitination, a modification well known to target membrane proteins to the lysosome or vacuole for degradation, can also function as recycling signal to sort a SNARE into COPI vesicles in a non-degradative pathway.
    Title: COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal.
  5. ubi4 repeats modulate proteostasis and stress survival
    Title: Variable repeats in the eukaryotic polyubiquitin gene ubi4 modulate proteostasis and stress survival.
  6. Both Ssa1 and Ssa2 are essential for the ubiquitin-dependent degradation of short-lived proteins and the requirements of Ssa proteins and the co-chaperones widely vary depending on the conformations and folding status of the substrates.
    Title: The requirements of yeast Hsp70 of SSA family for the ubiquitin-dependent degradation of short-lived and abnormal proteins.
  7. Zap1 controls UBI4 by inducing transcription from an intragenic promoter, and the resulting truncated mRNA encodes only two of the five ubiquitin repeats.
    Title: Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth.
  8. study provided evidence that S65 phosphorylation of ubiquitin is a biologically important modification that influences nearly every step of the in vivo ubiquitin life cycle
    Title: Phosphorylation of ubiquitin at Ser65 affects its polymerization, targets, and proteome-wide turnover.
  9. Expression of UbQ2N-E64G in stress hypersensitive SUB60 cells decreased growth rate and prolonged half-life of substrates of UFD pathway, besides failure of complementation under heat and antibiotic stresses, so Gln2 and Glu64 have been conserved.
    Title: Q2N and E64G double mutation of ubiquitin confers a stress sensitive phenotype on Saccharomyces cerevisiae.
  10. Ubiquitin signals proteolysis-independent stripping of transcription factors.
    Title: Ubiquitin signals proteolysis-independent stripping of transcription factors.
Submit: New GeneRIF Correction See all GeneRIFs (13)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables protein tag activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein tag activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein tag activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in modification-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in protein ubiquitination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in peroxisome IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
ubiquitin
NP_013061.1
  • Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via ubiquitin-26S proteasome system; essential for cellular stress response; encoded as polyubiquitin precursor comprised of 5 head-to-tail repeats; number of repeats is evolutionarily unstable within and between yeast species; protein abundance increases in response to DNA replication stress; implicated as modulator of replicative lifespan and meiotic recombination

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    64062..65207 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181859.1 → NP_013061.1  TPA: ubiquitin

    See identical proteins and their annotated locations for NP_013061.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VXW7, P0CG63
    UniProtKB/TrEMBL
    A7A0L9, B3LTD4, C8ZCT5, G2WIF8
    Conserved Domains (1) summary
    cd01803
    Location:77 → 152
    Ubl_ubiquitin; ubiquitin-like (Ubl) domain found in ubiquitin
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P0CG63.1

Gene LinkOut

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