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MSH3 mismatch repair protein MSH3 [ Saccharomyces cerevisiae S288C ]

Gene ID: 850454, updated on 30-Jul-2025
Official Symbol
MSH3
Official Full Name
mismatch repair protein MSH3
Primary source
SGD:S000000688
Locus tag
YCR092C
See related
AllianceGenome:SGD:S000000688; FungiDB:YCR092C; VEuPathDB:YCR092C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables DNA insertion or deletion binding activity and double-strand/single-strand DNA junction binding activity. Contributes to Y-form DNA binding activity and heteroduplex DNA loop binding activity. Involved in DNA metabolic process. Located in cytoplasm and nucleus. Part of MutSbeta complex. Used to study cancer. Human ortholog(s) of this gene implicated in colorectal carcinoma; endometrial cancer; endometrial carcinoma; familial adenomatous polyposis 4; and lung non-small cell carcinoma. Orthologous to human MSH3 (mutS homolog 3). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
all
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See MSH3 in Genome Data Viewer
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_001135.5 (276764..279820, complement)

Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene putative serine/threonine protein kinase KIN82 Neighboring gene CCR4-NOT core subunit CDC39 Neighboring gene aminophospholipid translocase regulatory protein CDC50

Genomic Sequence:
NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The sequence of a 6.3 kb segment of yeast chromosome III reveals an open reading frame coding for a putative mismatch binding protein. Valle G, et al. Yeast, 1991 Dec. PMID 1803822
  2. Distinct requirements within the Msh3 nucleotide binding pocket for mismatch and double-strand break repair. Kumar C, et al. J Mol Biol, 2013 Jun 12. PMID 23458407, Free PMC Article
  3. Mutations in the MSH3 gene preferentially lead to deletions within tracts of simple repetitive DNA in Saccharomyces cerevisiae. Strand M, et al. Proc Natl Acad Sci U S A, 1995 Oct 24. PMID 7479796, Free PMC Article
  4. Allele identification using immobilized mismatch binding protein: detection and identification of antibiotic-resistant bacteria and determination of sheep susceptibility to scrapie. Debbie P, et al. Nucleic Acids Res, 1997 Dec 1. PMID 9365263, Free PMC Article
  5. MSH3 Promotes Dynamic Behavior of Trinucleotide Repeat Tracts In Vivo. Williams GM, et al. Genetics, 2015 Jul. PMID 25969461, Free PMC Article

See all (147) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.
    Title: Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.
  2. Msh2-Msh3 hops over nucleosomes and other protein roadblocks, but maintains sufficient contact with DNA to recognize a single lesion.
    Title: Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesions.
  3. Trinucleotide Repeat (TNR) tracts were stabilized in the msh3Delta background, indicating that Msh2-Msh3 plays important role in shifting expansion-contraction equilibrium toward expansion in early stages of TNR tract expansion.
    Title: MSH3 Promotes Dynamic Behavior of Trinucleotide Repeat Tracts In Vivo.
  4. Data suggest that bot hATP and structure-specific repair substrates cooperate to direct Msh2-Msh3-mediated repair and provide an explanation for the msh3Y942A separation-of-function phenotype.
    Title: ATP binding and hydrolysis by Saccharomyces cerevisiae Msh2-Msh3 are differentially modulated by mismatch and double-strand break repair DNA substrates.
  5. a comparative study of Msh2-Msh3 and Msh2-Msh6 for mispair binding, sliding clamp formation, and Mlh1-Pms1 recruitment
    Title: Mispair-specific recruitment of the Mlh1-Pms1 complex identifies repair substrates of the Saccharomyces cerevisiae Msh2-Msh3 complex.
  6. Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease.
    Title: Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease.
  7. Authors identified a set of aromatic residues within the FLY motif of the predicted Msh3 nucleotide binding pocket that are essential for Msh2-Msh3-mediated mismatch repair but are largely dispensable for 3' non-homologous tail removal.
    Title: Distinct requirements within the Msh3 nucleotide binding pocket for mismatch and double-strand break repair.
  8. Msh2-Msh3 directly interferes with normal Okazaki fragment processing by flap endonuclease1 (Rad27) and DNA ligase I (Cdc9) in the presence of TNR sequences, thereby producing small, incremental expansion events
    Title: Msh2-Msh3 interferes with Okazaki fragment processing to promote trinucleotide repeat expansions.
  9. Data suggest that recognition of small insertion/deletion mispairs by Msh3, but not Msh2, requires more interaction with DNA conformations induced by small insertion/deletion mispairs than induced by large that are bent and strand separated.
    Title: Functional studies and homology modeling of Msh2-Msh3 predict that mispair recognition involves DNA bending and strand separation.
  10. Msh3-like behaviors beyond mispair specificity are not features controlled by the mispair-binding domain
    Title: Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins.
Submit: New GeneRIF Correction See all GeneRIFs (13)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent DNA damage sensor activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA insertion or deletion binding IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to Y-form DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables double-strand/single-strand DNA junction binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables double-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to heteroduplex DNA loop binding IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to mismatched DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables mismatched DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in meiotic mismatch repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mismatch repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mismatch repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mismatch repair IEA
Inferred from Electronic Annotation
more info
  
involved_in mismatch repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in removal of nonhomologous ends IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in removal of nonhomologous ends IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in replication fork arrest IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of MutSbeta complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm HDA PubMed 
located_in nucleus HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
mismatch repair protein MSH3
NP_010016.2
  • Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001135.5 Reference assembly

    Range
    276764..279820 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178798.1 → NP_010016.2  TPA: mismatch repair protein MSH3

    See identical proteins and their annotated locations for NP_010016.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A6ZTR3, D6VR92, P25336
    UniProtKB/TrEMBL
    N1P7Y1
    Conserved Domains (1) summary
    COG0249
    Location:127 → 1015
    MutS; DNA mismatch repair ATPase MutS [Replication, recombination and repair]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25336.2

Gene LinkOut

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