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BUD23 18S rRNA (guanine1575-N7)-methyltransferase [ Saccharomyces cerevisiae S288C ]

Gene ID: 850414, updated on 23-Apr-2026
Official Symbol
BUD23
Official Full Name
18S rRNA (guanine1575-N7)-methyltransferase
Primary source
SGD:S000000643
Locus tag
YCR047C
See related
AllianceGenome:SGD:S000000643; FungiDB:YCR047C; VEuPathDB:YCR047C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables rRNA (guanine) methyltransferase activity. Involved in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); rRNA (guanine-N7)-methylation; and ribosomal small subunit export from nucleus. Located in nucleolus. Human ortholog(s) of this gene implicated in Williams-Beuren syndrome. Orthologous to human BUD23 (BUD23 rRNA methyltransferase and ribosome maturation factor). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs
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See BUD23 in Genome Data Viewer
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_001135.5 (210718..211545, complement)

Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene Rrt12p Neighboring gene mitochondrial 54S ribosomal protein bL19m IMG1 Neighboring gene sterol acyltransferase Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein

Genomic Sequence:
NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. New phenotypes generated by the G57R mutation of BUD23 in Saccharomyces cerevisiae. Lin JL, et al. Yeast, 2012 Dec. PMID 23233232
  2. Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits. White J, et al. Mol Cell Biol, 2008 May. PMID 18332120, Free PMC Article
  3. Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast. Black JJ, et al. RNA, 2022 Mar. PMID 34934010, Free PMC Article
  4. Physical and functional interaction between the methyltransferase Bud23 and the essential DEAH-box RNA helicase Ecm16. Sardana R, et al. Mol Cell Biol, 2014 Jun. PMID 24710271, Free PMC Article
  5. The Stability of Ribosome Biogenesis Factor WBSCR22 Is Regulated by Interaction with TRMT112 via Ubiquitin-Proteasome Pathway. Õunap K, et al. PLoS One, 2015. PMID 26214185, Free PMC Article

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.
    Title: Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.
  2. Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.
    Title: Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae.
  3. BUD23 works at the transition of early to middle stage of 40S biogenesis.
    Title: The rRNA methyltransferase Bud23 shows functional interaction with components of the SSU processome and RNase MRP.
  4. The absence of Bud23, Bud23 (G57R) at a high level might affect the cell division of yeast cells through an as yet unidentified mechanism.
    Title: New phenotypes generated by the G57R mutation of BUD23 in Saccharomyces cerevisiae.
  5. Mutations in Bud23 that inactivated its methyltransferase activity complemented a bud23Delta mutant. In addition, mutant ribosomes in which G1575 was changed to adenosine supported growth comparable to that of cells with wild-type ribosomes.
    Title: Bud23 methylates G1575 of 18S rRNA and is required for efficient nuclear export of pre-40S subunits.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables S-adenosylmethionine-dependent methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA (guanine) methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (guanine) methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (guanine) methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA (guanine-N7)-methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in rRNA (guanine-N7)-methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA (guanine-N7)-methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA (guanine-N7)-methylation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosomal small subunit export from nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
18S rRNA (guanine1575-N7)-methyltransferase
NP_009976.1
  • Ribosome biogenesis factor; methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; required for recruitment of Rps2p; functions with DEAH-box RNA helicase Ecm16p; diploid mutant displays random budding pattern; functional homolog of human WBSCR22

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001135.5 Reference assembly

    Range
    210718..211545 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178761.1 → NP_009976.1  18S rRNA (guanine1575-N7)-methyltransferase

    See identical proteins and their annotated locations for NP_009976.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VR56, P25627
    UniProtKB/TrEMBL
    A6ZTM8, B3LUB2, C7GJT8, C8Z4D8, G2WA64, N1PAM9
    Conserved Domains (2) summary
    pfam12589
    Location:226 → 273
    WBS_methylT; Methyltransferase involved in Williams-Beuren syndrome
    pfam13649
    Location:51 → 152
    Methyltransf_25; Methyltransferase domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25627.1

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