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STE50 Ste50p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850325, updated on 23-Apr-2026
Official Symbol
STE50
Official Full Name
Ste50p
Primary source
SGD:S000000537
Locus tag
YCL032W
See related
AllianceGenome:SGD:S000000537; FungiDB:YCL032W; VEuPathDB:YCL032W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables SAM domain binding activity and protein kinase regulator activity. Involved in intracellular signal transduction; pheromone-dependent signal transduction involved in conjugation with cellular fusion; and signal transduction involved in filamentous growth. Acts upstream of or within establishment of cell polarity. Located in cytoplasm. [provided by Alliance of Genome Resources, Apr 2025]
Orthologs
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See STE50 in Genome Data Viewer
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_001135.5 (63441..64481)

Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene Lsb5p Neighboring gene peptide-methionine (R)-S-oxide reductase Neighboring gene Rrp7p Neighboring gene trifunctional histidinol dehydrogenase/phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase

Genomic Sequence:
NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The complete sequence of a 11,953 bp fragment from C1G on chromosome III encompasses four new open reading frames. Rad MR, et al. Yeast, 1991 Jul. PMID 1897318
  2. The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain. Sharmeen N, et al. Mol Biol Cell, 2019 Mar 15. PMID 30650049, Free PMC Article
  3. Polarization and cell-fate decision facilitated by the adaptor Ste50p in Saccharomyces cerevisiae. Sharmeen N, et al. PLoS One, 2022. PMID 36538537, Free PMC Article
  4. SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy. Slaughter BD, et al. PLoS One, 2008 Apr 23. PMID 18431466, Free PMC Article
  5. Structure of the sterile alpha motif (SAM) domain of the Saccharomyces cerevisiae mitogen-activated protein kinase pathway-modulating protein STE50 and analysis of its interaction with the STE11 SAM. Grimshaw SJ, et al. J Biol Chem, 2004 Jan 16. PMID 14573615

See all (119) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network.
    Title: Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor.
  2. Two peptide motifs within the intrinsically disordered cytoplasmic tail of Opy2p physically interact with the Step50p-RA domain.
    Title: Binding the atypical RA domain of Ste50p to the unfolded Opy2p cytoplasmic tail is essential for the high-osmolarity glycerol pathway.
  3. Ste50 has a role in control of MAPK specificity by feedback phosphorylation
    Title: Control of MAPK specificity by feedback phosphorylation of shared adaptor protein Ste50.
  4. inactivation of STE50 influences stress tolerance in mutants of the Ras-cAMP pathway, which is a major determinant of intrinsic stress tolerance and cell survival
    Title: Ste50 adaptor protein influences Ras/cAMP-driven stress-response and cell survival in Saccharomyces cerevisiae.
  5. propose that Opy2p can serve as a membrane anchor for the Ste50p/Ste11p module in the activation of the HOG pathway
    Title: Adaptor protein Ste50p links the Ste11p MEKK to the HOG pathway through plasma membrane association.
  6. Yeast bearing mutations that prevented the heterotypic Ste11-Ste50 association in vitro presented signaling defects in the mating and high-osmolarity growth pathways
    Title: Saccharomyces cerevisiae Ste50 binds the MAPKKK Ste11 through a head-to-tail SAM domain interaction.
  7. Results describe the solution structure of the sterile alpha motifs (SAM) domain of Ste11 and its interactions with the cognate SAM domain-containing region of Ste50.
    Title: Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex.
  8. Ste50 serves as an adaptor to tether Ste11 to the plasma membrane.
    Title: The RA domain of Ste50 adaptor protein is required for delivery of Ste11 to the plasma membrane in the filamentous growth signaling pathway of the yeast Saccharomyces cerevisiae.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables SAM domain binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase regulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within establishment of cell polarity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in osmosensory signaling pathway via Sho1 osmosensor IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in p38MAPK cascade IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in p38MAPK cascade IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in signal transduction IEA
Inferred from Electronic Annotation
more info
  
involved_in signal transduction involved in filamentous growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
Ste50p
NP_009898.1
  • Adaptor protein for various signaling pathways; involved in the mating response, invasive/filamentous growth and osmotolerance; acts as an adaptor that links the G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction; localizes to cortical patches at the site of shmoo initiation, facilitating polarity establishment, and remaining at the shmoo tip during mating

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001135.5 Reference assembly

    Range
    63441..64481
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178677.1 → NP_009898.1  Ste50p

    See identical proteins and their annotated locations for NP_009898.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQY3, P25344, Q3S1N2
    UniProtKB/TrEMBL
    A6ZTF8, B3LU50, B5VES6, C7GSY9, G2W9Z3, N1P660
    Conserved Domains (2) summary
    cd01786
    Location:226 → 325
    RA_STE50; Ras-associating (RA) domain found in the fungal adaptor protein STE50
    pfam09235
    Location:30 → 104
    Ste50p-SAM; Ste50p, sterile alpha motif
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25344.1

Gene LinkOut

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