KAR4 Kar4p [Saccharomyces cerevisiae S288C] - Gene

Summary

Official Symbol: KAR4
Official Full Name: Kar4p
Primary source: SGD:S000000560
Locus tag: YCL055W
See related: AllianceGenome:SGD:S000000560; FungiDB:YCL055W; VEuPathDB:YCL055W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity. Involved in karyogamy involved in conjugation with cellular fusion; meiotic cell cycle; and positive regulation of transcription by RNA polymerase II. Located in cytosol and nucleus. Part of RNA N6-methyladenosine methyltransferase complex. Orthologous to human METTL14 (methyltransferase 14, N6-adenosine-methyltransferase non-catalytic subunit). [provided by Alliance of Genome Resources, Jul 2025]
Orthologs: all
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Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis.
Title: Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis.
Kar4 is required for the normal pattern of meiotic gene expression.
Title: Kar4 is required for the normal pattern of meiotic gene expression.
Kar4 plays a critical role in the choreography of the mating response.
Title: Role of transcription factor Kar4 in regulating downstream events in the Saccharomyces cerevisiae pheromone response pathway.

Submit: New GeneRIF Correction

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in karyogamy	IEA Inferred from Electronic Annotation more info
involved_in karyogamy involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA modification	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of pseudohyphal growth	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RNA N6-methyladenosine methyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA N6-methyladenosine methyltransferase complex	IEA Inferred from Electronic Annotation more info
part_of RNA N6-methyladenosine methyltransferase complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of RNA N6-methyladenosine methyltransferase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

NP_009876.1

Transcription factor required for the mating response; subunit of the mRNA m6A methyltransferase (MIS) complex, regulating meiosis at multiple steps, early prior to pre-meiotic S phase and late during the completion of meiosis and sporulation; exists in two forms, a slower-migrating form abundant during vegetative growth and a faster-migrating pheromone-induced form; null mutant exhibits abnormal lipid metabolic phenotype; homologous to human METTL14, a subunit of a m6A methyltransferase complex